PMID- 31856256
OWN - NLM
STAT- MEDLINE
DCOM- 20200401
LR  - 20231027
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 12
DP  - 2019
TI  - Cytogenetics of the small-sized fish, Copeina guttata (Characiformes, 
      Lebiasinidae): Novel insights into the karyotype differentiation of the family.
PG  - e0226746
LID - 10.1371/journal.pone.0226746 [doi]
LID - e0226746
AB  - Lebiasinidae is a small fish family composed by miniature to small-sized fishes 
      with few cytogenetic data (most of them limited to descriptions of diploid 
      chromosome numbers), thus preventing any evolutionary comparative studies at the 
      chromosomal level. In the present study, we are providing, the first cytogenetic 
      data for the red spotted tetra, Copeina guttata, including the standard 
      karyotype, C-banding, repetitive DNA mapping by fluorescence in situ 
      hybridization (FISH) and comparative genomic hybridization (CGH), providing 
      chromosomal patterns and novel insights into the karyotype differentiation of the 
      family. Males and females share diploid chromosome number 2n = 42 and karyotype 
      composed of 2 metacentric (m), 4 submetacentric (sm) and 36 subtelocentric to 
      acrocentric (st-a) chromosomes. Blocks of constitutive heterochromatin were 
      observed in the centromeric and interstitial regions of several chromosomes, in 
      addition to a remarkably large distal block, heteromorphic in size, which fully 
      corresponded with the 18S rDNA sites in the fourth chromosomal pair. This overlap 
      was confirmed by 5S/18S rDNA dual-color FISH. On the other hand, 5S rDNA clusters 
      were situated in the long and short arms of the 2nd and 15th pairs, respectively. 
      No sex-linked karyotype differences were revealed by male/female CGH experiments. 
      The genomic probes from other two lebiasinid species, Lebiasina melanoguttata and 
      Pyrrhulina brevis, showed positive hybridization signals only in the NOR region 
      in the genome of C. guttata. We demonstrated that karyotype diversification in 
      lebiasinids was accompanied by a series of structural and numeric chromosome 
      rearrangements of different types, including particularly fusions and fissions.
FAU - Toma, Gustavo Akira
AU  - Toma GA
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
FAU - de Moraes, Renata Luiza Rosa
AU  - de Moraes RLR
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
FAU - Sassi, Francisco de Menezes Cavalcante
AU  - Sassi FMC
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
FAU - Bertollo, Luiz Antonio Carlos
AU  - Bertollo LAC
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
FAU - de Oliveira, Ezequiel Aguiar
AU  - de Oliveira EA
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
AD  - Secretaria de Estado de Educacao de Mato Grosso, Cuiaba, Mato Grosso, Brazil.
FAU - Rab, Petr
AU  - Rab P
AD  - Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech 
      Academy of Sciences, Libechov, Czech Republic.
FAU - Sember, Alexandr
AU  - Sember A
AD  - Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech 
      Academy of Sciences, Libechov, Czech Republic.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Institute of Human Genetics, University Hospital Jena, Jena, Germany.
FAU - Hatanaka, Terumi
AU  - Hatanaka T
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
FAU - Viana, Patrik Ferreira
AU  - Viana PF
AD  - Instituto Nacional de Pesquisas da Amazonia, Manaus, Amazonas, Brazil.
FAU - Marinho, Manoela Maria Ferreira
AU  - Marinho MMF
AD  - Museu de Zoologia da Universidade de Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Feldberg, Eliana
AU  - Feldberg E
AD  - Instituto Nacional de Pesquisas da Amazonia, Manaus, Amazonas, Brazil.
FAU - Cioffi, Marcelo de Bello
AU  - Cioffi MB
AUID- ORCID: 0000-0003-4340-1464
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, Sao Paulo, Brazil.
AD  - Institute of Human Genetics, University Hospital Jena, Jena, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191219
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Characiformes/classification/*genetics
MH  - Chromosomes/genetics
MH  - Evolution, Molecular
MH  - Female
MH  - *Karyotype
MH  - Male
MH  - *Phylogeny
MH  - RNA, Ribosomal/genetics
PMC - PMC6922430
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/12/20 06:00
MHDA- 2020/04/02 06:00
PMCR- 2019/12/19
CRDT- 2019/12/20 06:00
PHST- 2019/09/20 00:00 [received]
PHST- 2019/12/04 00:00 [accepted]
PHST- 2019/12/20 06:00 [entrez]
PHST- 2019/12/20 06:00 [pubmed]
PHST- 2020/04/02 06:00 [medline]
PHST- 2019/12/19 00:00 [pmc-release]
AID - PONE-D-19-26565 [pii]
AID - 10.1371/journal.pone.0226746 [doi]
PST - epublish
SO  - PLoS One. 2019 Dec 19;14(12):e0226746. doi: 10.1371/journal.pone.0226746. 
      eCollection 2019.