PMID- 31857964
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 2228-5881 (Print)
IS  - 2251-7308 (Electronic)
IS  - 2228-5881 (Linking)
VI  - 9
IP  - 4
DP  - 2019 Oct
TI  - Preparation and Characterization of Silk Fibroin Nanoparticles as a Potential 
      Drug Delivery System for 5-Fluorouracil.
PG  - 601-608
LID - 10.15171/apb.2019.069 [doi]
AB  - Purpose: The aim of this study is to prepare 5-fluorouracil (5-FU) loaded silk 
      fibroin nanoparticles (SFNPs) and to achieve a controlled release delivery system 
      with the high loading capacity. Methods: SFNPs with 1:1, 1:3, and 1:10 ratios of 
      5-FU to silk fibroin were prepared. SFNPs were characterized by Fourier-transform 
      infrared spectroscopy (FT-IR), X-ray diffraction (XRD) analysis, Scanning 
      electron microscope (SEM), and Transmission electron microscope (TEM). Loading 
      efficiency, in vitro release, and cell viability were studied for optimal SFNPs. 
      Results: The ratio of 1:1 was optimal formulation with the size and 
      polydispersity index (PDI) of 221.03 nm and 0.093 before freeze drying, and 286.7 
      nm and 0.154 after freeze drying by lactose, respectively. The loading efficiency 
      and loading content of this ratio were 52.32% and 34.35%, respectively. FT-IR and 
      XRD analysis indicated the conformational change (from random coil to beta-sheet) in 
      the structure of nanoparticles by increasing amount of the drug, which caused the 
      smaller size, the higher loading efficiency, and the slower release pattern. The 
      drugloaded nanoparticles reached to the half maximal inhibitory concentration 
      (IC50) that were comparable with free drug on MCF7 (human breast cancer) cell 
      line. Conclusion: This study was planned to achieve a promising controlled 
      release drug delivery system for carrying 5-FU, as a potent anticancer drug. 
      SFNPs were found proper candidates for delivery of a hydrophilic drug such as 
      5-FU.
CI  - (c) 2019 The Author (s).
FAU - Rahmani, Hamid
AU  - Rahmani H
AUID- ORCID: 0000-0001-5814-9703
AD  - Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University 
      of Medical Sciences, Kermanshah, 6734667149, Iran.
AD  - Student Research Committee, School of Pharmacy, Kermanshah University of Medical 
      Sciences, Kermanshah, 6734667149, Iran.
FAU - Fattahi, Ali
AU  - Fattahi A
AUID- ORCID: 0000-0001-8226-5835
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Sadrjavadi, Komail
AU  - Sadrjavadi K
AUID- ORCID: 0000-0002-2959-4215
AD  - Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University 
      of Medical Sciences, Kermanshah, 6734667149, Iran.
FAU - Khaledian, Salar
AU  - Khaledian S
AUID- ORCID: 0000-0003-2161-9200
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Shokoohinia, Yalda
AU  - Shokoohinia Y
AUID- ORCID: 0000-0003-3714-0835
AD  - Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University 
      of Medical Sciences, Kermanshah, 6734667149, Iran.
LA  - eng
PT  - Journal Article
DEP - 20191024
PL  - Iran
TA  - Adv Pharm Bull
JT  - Advanced pharmaceutical bulletin
JID - 101578021
PMC - PMC6912188
OTO - NOTNLM
OT  - 5-Fluorouracile
OT  - Cytotoxicity
OT  - Nanoparticle
OT  - Precipitation
OT  - SF
EDAT- 2019/12/21 06:00
MHDA- 2019/12/21 06:01
PMCR- 2019/10/24
CRDT- 2019/12/21 06:00
PHST- 2019/02/23 00:00 [received]
PHST- 2019/07/08 00:00 [revised]
PHST- 2019/07/21 00:00 [accepted]
PHST- 2019/12/21 06:00 [entrez]
PHST- 2019/12/21 06:00 [pubmed]
PHST- 2019/12/21 06:01 [medline]
PHST- 2019/10/24 00:00 [pmc-release]
AID - 10.15171/apb.2019.069 [doi]
PST - ppublish
SO  - Adv Pharm Bull. 2019 Oct;9(4):601-608. doi: 10.15171/apb.2019.069. Epub 2019 Oct 
      24.