PMID- 31858563
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20201215
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 24
DP  - 2019 Dec
TI  - MiR-205 influences renal injury in sepsis rats through HMGB1-PTEN signaling 
      pathway.
PG  - 10950-10956
LID - 19798 [pii]
LID - 10.26355/eurrev_201912_19798 [doi]
AB  - OBJECTIVE: To investigate the influences of micro ribonucleic acid (miR)-205 on 
      renal injury in sepsis rats through the high-mobility group box 1 
      (HMGB1)-phosphatase and tensin homolog deleted on chromosome ten (PTEN) signaling 
      pathway. MATERIALS AND METHODS: A rat model of sepsis-induced renal injury was 
      established by cecal ligation and perforation. The rats were randomly divided 
      into 3 groups, namely the Sham group, the Model group, and the miR-205 group. 
      Hematoxylin and eosin (HE) staining was applied to examine the pathological renal 
      morphology. The enzyme-linked immunosorbent assay (ELISA) was adopted to measure 
      the serum levels of Caspase-3 and Bcl-2-associated X protein (Bax) in rats. Cell 
      apoptosis rate in the renal tissues was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. Finally, the protein 
      levels of phosphorylate-HMGB1 (p-HMGB1) and p-PTEN in the renal tissues were 
      determined using the Western blotting (WB) assay. RESULTS: Compared with those in 
      the Sham group, the pathological morphology of the renal tissues was poor in 
      Model group. The serum levels of Caspase-3 and Bax, the apoptosis rate, and the 
      protein levels of p-HMGB1 and p-PTEN were remarkably enhanced in the Model group 
      compared to the Sham group. In comparison with those in Model group, the 
      pathological changes in renal morphology, apoptosis-related indexes, and protein 
      levels of p-HMGB1 and p-PTEN were alleviated in the miR-205 group. CONCLUSIONS: 
      MiR-205 agonist can improve the pathological morphology in the sepsis rats with 
      renal injury, improve renal cell apoptosis, and inhibit the protein levels of 
      HMGB1 and PTEN in renal tissues. MiR-205 alleviates sepsis-induced renal injury 
      through the HMGB1-PTEN signaling pathway.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Department of Critical Care Medicine, the Affiliated Suzhou Hospital of Nanjing 
      Medical University, Suzhou, China. 815381426@qq.com.
FAU - Xia, F
AU  - Xia F
FAU - Wu, J
AU  - Wu J
FAU - Yang, A-X
AU  - Yang AX
FAU - Zhang, Y-Y
AU  - Zhang YY
FAU - Zhao, H
AU  - Zhao H
FAU - Tao, W-Y
AU  - Tao WY
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (HMGB1 Protein)
RN  - 0 (Hbp1 protein, rat)
RN  - 0 (MIRN205 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (Pten protein, rat)
SB  - IM
MH  - Acute Kidney Injury/*metabolism/pathology
MH  - Animals
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - HMGB1 Protein/analysis/*metabolism
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - PTEN Phosphohydrolase/analysis/*metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sepsis/*metabolism/pathology
MH  - *Signal Transduction
EDAT- 2019/12/21 06:00
MHDA- 2020/12/16 06:00
CRDT- 2019/12/21 06:00
PHST- 2019/12/21 06:00 [entrez]
PHST- 2019/12/21 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
AID - 19798 [pii]
AID - 10.26355/eurrev_201912_19798 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Dec;23(24):10950-10956. doi: 
      10.26355/eurrev_201912_19798.