PMID- 31858644
OWN - NLM
STAT- MEDLINE
DCOM- 20200826
LR  - 20200826
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 55
IP  - 2
DP  - 2020 Feb
TI  - Abnormal functions of Leydig cells in crossbred cattle-yak showing infertility.
PG  - 209-216
LID - 10.1111/rda.13609 [doi]
AB  - In Mongolia, yak (Bos grunniens) are able to live in alpine areas and their 
      products greatly influence the lives of the local people. Increased vigour in 
      hybridized yak and cattle can offer benefits for livestock farmers. However, male 
      hybrids show reproductive defects resulting from spermatogenesis arrest, 
      affecting the conservation and maintenance of dominant traits in the next 
      generation. The underlying mechanisms involved in hybrid cattle-yak infertility 
      have recently been investigated; however, the genetic cause is still unclear. 
      Androgens and androgen receptor (AR) signalling are required for spermatogenesis. 
      We, therefore, evaluated the expression of AR, 3beta-hydroxysteroid dehydrogenase 
      (3betaHSD) and 5alpha-reductase 2 (SRD5A2) in Leydig cells to investigate their function 
      in cattle-yak spermatogenesis. Testicular tissues from yaks (1-3 years old) and 
      hybrids (F1-F3, 2 years old) were collected and subjected to immunohistochemistry 
      and image analyses to investigate the expression of each parameter in the Leydig 
      cells. After maturation at 2 years, the expression levels of AR increased and the 
      levels of 3betaHSD decreased, but the SRD5A2 levels remained constant in yak. 
      However, the cattle-yak hybrid F2 showed immature testicular development and 
      significantly different expression levels of AR and 3betaHSD compared with mature 
      yak. These results suggest that the decreased expression of AR and increased 
      expression of 3betaHSD in the Leydig cells of cattle-yak hybrid testes may represent 
      one of the causes of infertility. Our study might help in solving the problem of 
      infertility in crossbreeding.
CI  - (c) 2019 Blackwell Verlag GmbH.
FAU - Sato, Yoko
AU  - Sato Y
AUID- ORCID: 0000-0003-1683-8505
AD  - Department of Medical Engineering, Faculty of Allied Sciences, University of East 
      Asia, Yamaguchi, Japan.
FAU - Kuriwaki, Ryota
AU  - Kuriwaki R
AD  - Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, Yamaguchi, Japan.
FAU - Hagino, Shiki
AU  - Hagino S
AD  - Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, Yamaguchi, Japan.
FAU - Shimazaki, Megumi
AU  - Shimazaki M
AD  - Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, Yamaguchi, Japan.
FAU - Sambuu, Rentsenkhand
AU  - Sambuu R
AD  - Institute for Extension of Agricultural Advanced Technology, Ulaanbaatar, 
      Mongolia.
FAU - Hirata, Maki
AU  - Hirata M
AUID- ORCID: 0000-0003-4673-1581
AD  - Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
FAU - Tanihara, Fuminori
AU  - Tanihara F
AUID- ORCID: 0000-0002-2158-5808
AD  - Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
FAU - Takagi, Mitsuhiro
AU  - Takagi M
AUID- ORCID: 0000-0001-9298-8287
AD  - Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, Yamaguchi, Japan.
FAU - Taniguchi, Masayasu
AU  - Taniguchi M
AUID- ORCID: 0000-0003-3422-1904
AD  - Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, Yamaguchi, Japan.
FAU - Otoi, Takeshige
AU  - Otoi T
AUID- ORCID: 0000-0002-0382-959X
AD  - Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan.
LA  - eng
GR  - 25304039/Ministry of Education/
PT  - Journal Article
DEP - 20200121
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Receptors, Androgen)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/metabolism
MH  - Animals
MH  - Cattle/*genetics
MH  - *Hybridization, Genetic
MH  - Infertility, Male/*genetics/pathology
MH  - Leydig Cells/enzymology/*metabolism
MH  - Male
MH  - Receptors, Androgen/metabolism
MH  - Spermatogenesis/genetics
MH  - Testis/growth & development
OTO - NOTNLM
OT  - Leydig cell
OT  - androgen receptor
OT  - cattle-yak
OT  - hybrid
OT  - spermatogenesis
OT  - yak
EDAT- 2019/12/21 06:00
MHDA- 2020/08/28 06:00
CRDT- 2019/12/21 06:00
PHST- 2019/10/28 00:00 [received]
PHST- 2019/12/16 00:00 [accepted]
PHST- 2019/12/21 06:00 [pubmed]
PHST- 2020/08/28 06:00 [medline]
PHST- 2019/12/21 06:00 [entrez]
AID - 10.1111/rda.13609 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2020 Feb;55(2):209-216. doi: 10.1111/rda.13609. Epub 2020 Jan 
      21.