PMID- 31861478
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20200518
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 1
DP  - 2019 Dec 18
TI  - Exogenous Melatonin and Abscisic Acid Expedite the Flavonoids Biosynthesis in 
      Grape Berry of Vitis vinifera cv. Kyoho.
LID - 10.3390/molecules25010012 [doi]
LID - 12
AB  - Grape polyphenols contributing to more than half of the global polyphenol market 
      were well studied; however, how melatonin (MLT), a potential plant hormone, and 
      abscisic acid (ABA) affects polyphenols profile is still poorly understood. To 
      explore whether these hormones are involved in polyphenolic biosynthesis, grape 
      (Vitis vinifera cv. Kyoho) was exposed to MLT, ABA, and NDGA 
      (nordihydroguaiaretic acid, an ABA biosynthesis inhibitor) treatments, and 16 
      polyphenols were identified from grape extracts by high performance liquid 
      chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS). Both 
      exogenous MLT and ABA significantly enhanced the biosynthesis of each flavonol 
      and flavanol component, especially catechin, which was almost increased double by 
      200 microM of MLT treatment. Furthermore, the expression of genes involved in 
      flavonoid biosynthesis, including 4-coumaroyl-CoA synthase, chalcone synthase, 
      flavonoid 3'-hydroxylase, anthocyanin 3'-methyltransferase, flavonol synthase, 
      flavonoid-3-O-glucosyltransferase, and flavonoid 3',5'-methyltransferase were 
      highly up-regulated as well but were down-regulated by NDGA. The present study 
      provided new insights for improving flavonoids accumulation in agricultural 
      production and its underlying mechanism.
FAU - Yang, Mingyi
AU  - Yang M
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
FAU - Belwal, Tarun
AU  - Belwal T
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
FAU - Zhang, Xiaocheng
AU  - Zhang X
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
FAU - Lu, Hongyan
AU  - Lu H
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
FAU - Chen, Cunkun
AU  - Chen C
AD  - National Engineering Technology Research Center for Preservation of Agricultural 
      Products, Key Laboratory of Postharvest Physiology and Storage of Agricultural 
      Products, Ministry of Agriculture of China, Tianjin 300112, China.
FAU - Li, Li
AU  - Li L
AUID- ORCID: 0000-0002-1242-3866
AD  - Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, 
      Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems 
      Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
AD  - Ningbo Research Institute, Zhejiang University, Ningbo 315100, China.
AD  - National-Local Joint Engineering Laboratory of Intelligent Food Technology and 
      Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang Engineering 
      Laboratory of Food Technology and Equipment, Zhejiang University, Hangzhou 
      310058, China.
LA  - eng
GR  - 2017YFD0401304/National Key Research and Development Program of China/
GR  - 31571895, 31772366/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20191218
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Flavonoids)
RN  - 0 (Polyphenols)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Abscisic Acid/*pharmacology
MH  - Flavonoids/*analysis/*biosynthesis
MH  - Fruit/*chemistry
MH  - Gene Expression Regulation, Plant
MH  - Mass Spectrometry
MH  - Melatonin/*pharmacology
MH  - Polyphenols/analysis/biosynthesis
MH  - Vitis/*chemistry/genetics
PMC - PMC6983125
OTO - NOTNLM
OT  - Vitis vinifera
OT  - abscisic acid
OT  - flavonoid
OT  - grape
OT  - melatonin
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/22 06:00
MHDA- 2020/05/19 06:00
PMCR- 2019/12/18
CRDT- 2019/12/22 06:00
PHST- 2019/11/13 00:00 [received]
PHST- 2019/12/09 00:00 [revised]
PHST- 2019/12/11 00:00 [accepted]
PHST- 2019/12/22 06:00 [entrez]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2019/12/18 00:00 [pmc-release]
AID - molecules25010012 [pii]
AID - molecules-25-00012 [pii]
AID - 10.3390/molecules25010012 [doi]
PST - epublish
SO  - Molecules. 2019 Dec 18;25(1):12. doi: 10.3390/molecules25010012.