PMID- 31861704
OWN - NLM
STAT- MEDLINE
DCOM- 20200504
LR  - 20200505
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 1
DP  - 2019 Dec 19
TI  - Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cell Culture 
      Prevents UVB-Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts.
LID - 10.3390/ijms21010049 [doi]
LID - 49
AB  - Human adipose-derived mesenchymal stem cells-conditioned medium (ADSC-CM) 
      contains cytokines and growth factors that can facilitate the regeneration and 
      repair of various tissues and organs. In the present study, the protective 
      activity of ADSC-CM treatment was investigated in UVB-irradiated human 
      keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs). It was 
      found that ADSC-CM can modulate the expression of the signaling molecules in the 
      early UVB responsive signaling pathways, including mitogen activated protein 
      kinases (MAPKs), activator protein 1 (AP-1), and nuclear factor kappa B (NF-kappaB). 
      In addition, ADSC-CM treatment could upregulate antioxidant response element 
      (ARE) such as phase II gene heme oxygenase-1 (HO-1) and increase the expression 
      of collagen synthesis enhancer gene transforming growth factor-beta (TGF-beta). The 
      expression of matrix metalloproteinase-1 (MMP-1) and procollagen type I synthesis 
      inhibitors such as interleukin-6 (IL-6) was also found to be suppressed upon 
      ADSC-CM treatment. Taken together, our study illustrates the anti-photoaging 
      activities of ADSC-CM in cell-based models.
FAU - Li, Lu
AU  - Li L
AD  - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 
      Shanghai SH021, China.
AD  - College of Life Sciences, Kyung Hee University, 1732, Deogyeongdae-ro, 
      Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea.
FAU - Ngo, Hien T T
AU  - Ngo HTT
AD  - College of Life Sciences, Kyung Hee University, 1732, Deogyeongdae-ro, 
      Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea.
FAU - Hwang, Eunson
AU  - Hwang E
AD  - College of Life Sciences, Kyung Hee University, 1732, Deogyeongdae-ro, 
      Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea.
FAU - Wei, Xuan
AU  - Wei X
AD  - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 
      Shanghai SH021, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - College of Life Sciences, Kyung Hee University, 1732, Deogyeongdae-ro, 
      Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea.
FAU - Liu, Jia
AU  - Liu J
AD  - Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, 
      Shanghai SH021, China.
FAU - Yi, Tae-Hoo
AU  - Yi TH
AD  - College of Life Sciences, Kyung Hee University, 1732, Deogyeongdae-ro, 
      Giheung-gu, Yongin-si, Gyeonggi-do 17104, Korea.
LA  - eng
PT  - Journal Article
DEP - 20191219
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Adipose Tissue/*cytology/metabolism
MH  - Cell Culture Techniques
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/*pharmacology
MH  - Cytokines/metabolism
MH  - Fibroblasts/cytology/drug effects/radiation effects
MH  - Gene Expression Regulation/drug effects/radiation effects
MH  - Humans
MH  - Keratinocytes/*cytology/drug effects/radiation effects
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Signal Transduction/drug effects/radiation effects
MH  - Skin Aging/*drug effects
MH  - Ultraviolet Rays/adverse effects
PMC - PMC6981944
OTO - NOTNLM
OT  - MAPK/AP-1
OT  - NF-kappaB/HO-1
OT  - TGF-beta/SMAD
OT  - UVB radiation
OT  - human adipose-derived mesenchymal stem cells-conditioned medium
COIS- All authors declare no conflicts of interests.
EDAT- 2019/12/22 06:00
MHDA- 2020/05/06 06:00
PMCR- 2020/01/01
CRDT- 2019/12/22 06:00
PHST- 2019/09/30 00:00 [received]
PHST- 2019/11/09 00:00 [revised]
PHST- 2019/11/14 00:00 [accepted]
PHST- 2019/12/22 06:00 [entrez]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - ijms21010049 [pii]
AID - ijms-21-00049 [pii]
AID - 10.3390/ijms21010049 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Dec 19;21(1):49. doi: 10.3390/ijms21010049.