PMID- 31861881
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 8
IP  - 1
DP  - 2019 Dec 20
TI  - Dendritic Cells Internalize Staphylococcus aureus More Efficiently than 
      Staphylococcus epidermidis, but Do Not Differ in Induction of Antigen-Specific T 
      Cell Proliferation.
LID - 10.3390/microorganisms8010019 [doi]
LID - 19
AB  - Staphylococcus aureus and Staphylococcus epidermidis are related species which 
      can cause predominantly acute and subacute infections, respectively. Differences 
      in human adaptive immune responses to these two species are not well understood. 
      Dendritic cells (DCs) have an important role in the control and regulation of 
      anti-staphylococcal T cell responses. Therefore, we aimed to compare the ability 
      of S. aureus and S. epidermidis to influence the essential steps in human DC 
      activation and subsequent antigen-specific CD4(+) T cell proliferation, and to 
      investigate the underlying mechanisms. Using multiple strains of both species, we 
      observed that S. aureus was internalized more effectively than S. epidermidis by 
      DCs but that both species were equally potent in activating these host cells, as 
      evidenced by similar induction of DC maturation marker expression and antigen 
      loading onto MHC-II molecules. The DCs stimulated by S. aureus strains not 
      harboring superantigen (SAg) genes or by any of the S. epidermidis strains, 
      induced low, likely physiological levels of T cell proliferation. Only DCs 
      stimulated with S. aureus strains harboring SAg genes induced high levels of T 
      cell proliferation. Taken together, S. aureus and S. epidermidis do not 
      differently affect DC activation and ensuing antigen-specific T cell 
      proliferation, unless a strain has the capacity to produce SAgs.
FAU - Balraadjsing, Payal P
AU  - Balraadjsing PP
AD  - Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, 
      Amsterdam UMC, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The 
      Netherlands.
FAU - de Jong, Esther C
AU  - de Jong EC
AD  - Department of Experimental Immunology, Amsterdam Infection and Immunity 
      Institute, Amsterdam UMC, University of Amsterdam, Meibergdreef 15, 1105 AZ 
      Amsterdam, The Netherlands.
FAU - van Wamel, Willem J B
AU  - van Wamel WJB
AD  - Department of Medical Microbiology and Infectious Diseases, Erasmus Medical 
      Center, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands.
FAU - Zaat, Sebastian A J
AU  - Zaat SAJ
AD  - Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, 
      Amsterdam UMC, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The 
      Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20191220
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC7022728
OTO - NOTNLM
OT  - Staphylococcus aureus
OT  - Staphylococcus epidermidis
OT  - T cells
OT  - dendritic cells
OT  - human immune response
OT  - superantigen
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/22 06:00
MHDA- 2019/12/22 06:01
PMCR- 2019/12/20
CRDT- 2019/12/22 06:00
PHST- 2019/10/03 00:00 [received]
PHST- 2019/12/05 00:00 [revised]
PHST- 2019/12/18 00:00 [accepted]
PHST- 2019/12/22 06:00 [entrez]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2019/12/22 06:01 [medline]
PHST- 2019/12/20 00:00 [pmc-release]
AID - microorganisms8010019 [pii]
AID - microorganisms-08-00019 [pii]
AID - 10.3390/microorganisms8010019 [doi]
PST - epublish
SO  - Microorganisms. 2019 Dec 20;8(1):19. doi: 10.3390/microorganisms8010019.