PMID- 31862647
OWN - NLM
STAT- MEDLINE
DCOM- 20200702
LR  - 20200930
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 245
DP  - 2020 Feb
TI  - Factors regulating the differential uptake of persistent organic pollutants in 
      cucurbits and non-cucurbits.
PG  - 153094
LID - S0176-1617(19)30223-8 [pii]
LID - 10.1016/j.jplph.2019.153094 [doi]
AB  - Contamination with persistent organic pollutants (POPs) has become a worldwide 
      concern owing to their the toxicity to humans and wildlife. Pumpkin, cucumber, 
      and squash (Cucurbitaceae) accumulate POPs in their shoots in concentrations 
      higher than those in non-cucurbits; to elucidate the underlying molecular 
      mechanisms of this accumulation, POP transporters were analyzed in the xylem sap 
      of cucurbits and non-cucurbits. The 17-kDa xylem sap proteins detected in all 
      cucurbits but not in non-cucurbits readily bound polychlorinated biphenyl (PCB) 
      in all tested cucurbits, except in cucumber and loofah, and to dieldrin in all 
      tested cucurbits. Ten genes encoding major latex-like proteins (MLPs) responsible 
      for the accumulation of PCBs in zucchini plants were cloned from cucurbits. 
      Phylogenetic analysis using MLP sequences identified two separate clades, one 
      containing Cucurbitaceae MLPs and the other containing those of non-cucurbit 
      members. Recombinant MLPs bound PCB and dieldrin. Western blotting with anti-MLP 
      antibodies identified translocatable and non-translocatable MLPs between root and 
      stem xylem vessels. Translocation of MLPs from the root to stem xylem vessels and 
      POP-binding ability of MLPs are important for selective accumulation of MLPs in 
      cucurbits. This study provides basic knowledge about phytoremediation through 
      overexpression of MLP genes and for breeding cucurbits that accumulate less 
      contaminants.
CI  - Copyright (c) 2019 Elsevier GmbH. All rights reserved.
FAU - Iwabuchi, Aya
AU  - Iwabuchi A
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo 657-8501, Japan.
FAU - Katte, Nonoka
AU  - Katte N
AD  - Faculty of Agriculture, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 
      657-8501, Japan.
FAU - Suwa, Mizuki
AU  - Suwa M
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo 657-8501, Japan.
FAU - Goto, Junya
AU  - Goto J
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo 657-8501, Japan.
FAU - Inui, Hideyuki
AU  - Inui H
AD  - Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodaicho, 
      Nada-ku, Kobe, Hyogo 657-8501, Japan; Biosignal Research Center, Kobe University, 
      1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Electronic address: 
      hinui@kobe-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20191211
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Environmental Pollutants)
RN  - 0 (Plant Proteins)
RN  - 0 (Soil Pollutants)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Biological Transport/genetics/physiology
MH  - Cucurbitaceae/*metabolism
MH  - Environmental Pollutants/chemistry/metabolism
MH  - Gene Expression/genetics
MH  - Phylogeny
MH  - Plant Proteins/genetics/isolation & purification/*metabolism
MH  - Plant Roots/metabolism
MH  - Plants, Genetically Modified
MH  - Polychlorinated Biphenyls/metabolism
MH  - Protein Binding/genetics
MH  - Soil Pollutants/chemistry/*metabolism
MH  - Xylem/metabolism
OTO - NOTNLM
OT  - Crop contamination
OT  - Cucurbitaceae
OT  - Major latex-like protein
OT  - Persistent organic pollutant
OT  - Polychlorinated biphenyl
OT  - Xylem sap protein
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2019/12/22 06:00
MHDA- 2020/07/03 06:00
CRDT- 2019/12/22 06:00
PHST- 2019/08/25 00:00 [received]
PHST- 2019/11/08 00:00 [revised]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2020/07/03 06:00 [medline]
PHST- 2019/12/22 06:00 [entrez]
AID - S0176-1617(19)30223-8 [pii]
AID - 10.1016/j.jplph.2019.153094 [doi]
PST - ppublish
SO  - J Plant Physiol. 2020 Feb;245:153094. doi: 10.1016/j.jplph.2019.153094. Epub 2019 
      Dec 11.