PMID- 31863943
OWN - NLM
STAT- MEDLINE
DCOM- 20200909
LR  - 20200909
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 182
DP  - 2020 Mar
TI  - Simultaneous exploration of nutrients and pollutants in human milk and their 
      impact on preterm infant growth: An integrative cross-platform approach.
PG  - 109018
LID - S0013-9351(19)30815-1 [pii]
LID - 10.1016/j.envres.2019.109018 [doi]
AB  - Early nutritional management including fortified human breastmilk is currently 
      recommended to fulfil the energy demands and counterbalance risks associated to 
      preterm birth. However, little is known about the potential adverse effects of 
      exposure to persistent organic pollutants (POPs) carried in human milk on preterm 
      infant growth. We conducted a pilot study proving the application of an 
      integrative analytical approach based on mass spectrometry (MS) coupled to 
      advanced statistical models, favouring the comprehensive molecular profiling to 
      support the identification of multiple biomarkers. We applied this workflow in 
      the frame of a preterm infants' cohort to explore environmental determinants of 
      growth. The combination of high resolution gas and liquid chromatography MS 
      platforms generated a large molecular profile, including 102 pollutants and 
      nutrients (targeted analysis) and 784 metabolites (non-targeted analysis). Data 
      analysis consisted in a preliminary examination of associations between the 
      signatures of POPs and the normalised growth of preterm infants, using 
      multivariate linear regression adjusting for known confounding variables. A 
      second analysis aimed to identify multidimensional biomarkers using a multiblock 
      algorithm allowing the integration of multiple datasets in the growth model of 
      preterm infants. The preliminary results did not suggest an impairment of preterm 
      growth associated to the milk concentrations of POPs. The multiblock approach 
      however revealed complex interrelated molecular networks of POPs, lipids, 
      metabolites and amino acids in breastmilk associated to preterm infant growth, 
      supporting the high potential of biomarkers exploration of this proposed 
      workflow. Whereas the present study intended to identify simultaneously pollutant 
      and nutrient exposure profiles associated to early preterm infant growth, this 
      workflow may be easily adapted and applied to other matrices (e.g. serum) and 
      research settings, favouring the functional exploration of environmental 
      determinants of complex and multifactorial diseases.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Cano-Sancho, German
AU  - Cano-Sancho G
AD  - LABERCA, Oniris, INRA, 44307, Nantes, France. Electronic address: 
      laberca@oniris-nantes.fr.
FAU - Alexandre-Gouabau, Marie-Cecile
AU  - Alexandre-Gouabau MC
AD  - Nantes Universite, INRA, UMR1280, Physiopathologie des Adaptations 
      Nutritionnelles, Centre de Recherche en Nutrition Humaine Ouest (CRNH-Ouest), 
      Institut des Maladies de L'appareil Digestif (IMAD), F-44000, Nantes, France.
FAU - Moyon, Thomas
AU  - Moyon T
AD  - Nantes Universite, INRA, UMR1280, Physiopathologie des Adaptations 
      Nutritionnelles, Centre de Recherche en Nutrition Humaine Ouest (CRNH-Ouest), 
      Institut des Maladies de L'appareil Digestif (IMAD), F-44000, Nantes, France.
FAU - Royer, Anne-Lise
AU  - Royer AL
AD  - LABERCA, Oniris, INRA, 44307, Nantes, France.
FAU - Guitton, Yann
AU  - Guitton Y
AD  - LABERCA, Oniris, INRA, 44307, Nantes, France.
FAU - Billard, Helene
AU  - Billard H
AD  - Nantes Universite, INRA, UMR1280, Physiopathologie des Adaptations 
      Nutritionnelles, Centre de Recherche en Nutrition Humaine Ouest (CRNH-Ouest), 
      Institut des Maladies de L'appareil Digestif (IMAD), F-44000, Nantes, France.
FAU - Darmaun, Dominique
AU  - Darmaun D
AD  - Nantes Universite, INRA, UMR1280, Physiopathologie des Adaptations 
      Nutritionnelles, Centre de Recherche en Nutrition Humaine Ouest (CRNH-Ouest), 
      Institut des Maladies de L'appareil Digestif (IMAD), F-44000, Nantes, France.
FAU - Roze, Jean-Christophe
AU  - Roze JC
AD  - CHU, Centre Hospitalo-Universitaire Hotel-Dieu, Nantes, France.
FAU - Boquien, Clair-Yves
AU  - Boquien CY
AD  - Nantes Universite, INRA, UMR1280, Physiopathologie des Adaptations 
      Nutritionnelles, Centre de Recherche en Nutrition Humaine Ouest (CRNH-Ouest), 
      Institut des Maladies de L'appareil Digestif (IMAD), F-44000, Nantes, France; 
      EMBA, European Milk Bank Association, Milano, Italy.
FAU - Le Bizec, Bruno
AU  - Le Bizec B
AD  - LABERCA, Oniris, INRA, 44307, Nantes, France.
FAU - Antignac, Jean-Philippe
AU  - Antignac JP
AD  - LABERCA, Oniris, INRA, 44307, Nantes, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191213
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - 0 (Environmental Pollutants)
SB  - IM
MH  - *Child Development/drug effects
MH  - *Environmental Pollutants/toxicity
MH  - Humans
MH  - Infant
MH  - Infant Nutritional Physiological Phenomena
MH  - Infant, Newborn
MH  - *Infant, Premature
MH  - *Milk, Human/chemistry
MH  - Nutrients
MH  - Pilot Projects
OTO - NOTNLM
OT  - Biomarkers
OT  - Breast milk
OT  - Exposome
OT  - Nutrients
OT  - Persistent pollutants
OT  - Preterm infants
OT  - Risk-benefit assessment
EDAT- 2019/12/22 06:00
MHDA- 2020/09/10 06:00
CRDT- 2019/12/22 06:00
PHST- 2019/09/30 00:00 [received]
PHST- 2019/11/19 00:00 [revised]
PHST- 2019/12/06 00:00 [accepted]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2020/09/10 06:00 [medline]
PHST- 2019/12/22 06:00 [entrez]
AID - S0013-9351(19)30815-1 [pii]
AID - 10.1016/j.envres.2019.109018 [doi]
PST - ppublish
SO  - Environ Res. 2020 Mar;182:109018. doi: 10.1016/j.envres.2019.109018. Epub 2019 
      Dec 13.