PMID- 31864010
OWN - NLM
STAT- MEDLINE
DCOM- 20201110
LR  - 20201110
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 143
DP  - 2020 Feb
TI  - Expression and functional role of fibroblast growth factors (FGF) in placenta 
      during different stages of pregnancy in water buffalo (Bubalus bubalis).
PG  - 98-112
LID - S0093-691X(19)30533-3 [pii]
LID - 10.1016/j.theriogenology.2019.11.034 [doi]
AB  - The present study documented the expression and functional role of Fibroblast 
      growth factors (FGFs) family and their receptors (Fibroblast growth factor 
      receptor, FGFRs) in placenta (Cotyledon; COT, Caruncle; CAR) during different 
      stages of pregnancy in water buffalo. Samples were collected from Early pregnancy 
      1 (EP1); Early pregnancy 2 (EP2); Mid pregnancy (MP) and Late pregnancy (LP) 
      while diestrus stage of oestrus cycle (NP) was taken as control. In addition, 
      modulatory role of FGF2 on mRNA expression of von Willebrand factor (vWF), 
      Proliferating cell nuclear antigen (PCNA), steroidogenic acute regulatory protein 
      (StAR), cytochrome P450 cholesterol side-chain cleavage (CYP11A1), 
      3beta-hydroxysteroid dehydrogenase (3betaHSD) and BCL2 Associated X (BAX) were studied 
      in cultured trophoblast cells (TCC), obtained from EP2. Real-time PCR (qPCR), 
      Western blot, and immunohistochemistry were applied to investigate mRNA and 
      protein expressions, and the localization of examined factors whereas, P4 
      secretion was assessed by RIA. The mRNA and protein expression of FGFs and its 
      receptors were maximum (P < 0.05) during EP (EP1 and EP2) in COT. However, FGFR1 
      and FGFR4 were upregulated (P < 0.05) during EP2 and MP in COT. Similarly, the 
      mRNA and protein expression of FGFs and its receptors were upregulated (P < 0.05) 
      during all stages of pregnancy in CAR. FGF family members were localized in the 
      cytoplasm of trophoblast cells as well as in fetal blood vessels. At 100 ng/ml 
      dosage, FGF2 stimulated the transcript of vWF maximally (P < 0.05). P4 secretion 
      in trophoblast cells treated with FGF2 was maximum with the highest dose at 72 h. 
      These findings corroborate that FGF acts locally in the trophoblast cells to 
      modulate steroid hormone viz. progesterone synthesis, promote angiogenesis and 
      favors cell survivability indicating that this factor may play an essential role 
      in the regulation of placental formation and function in buffalo.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Devi, H Lakshmi
AU  - Devi HL
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Kumar, S
AU  - Kumar S
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Konyak, Y Y
AU  - Konyak YY
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Bharati, Jaya
AU  - Bharati J
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Bhimte, A
AU  - Bhimte A
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Pandey, Y
AU  - Pandey Y
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Kumar, K
AU  - Kumar K
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Paul, A
AU  - Paul A
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Kala, Anju
AU  - Kala A
AD  - Animal Nutrition Division, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Samad, H A
AU  - Samad HA
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Verma, M R
AU  - Verma MR
AD  - Division of Livestock Economics, Statistics and Information Technology, Indian 
      Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
FAU - Singh, G
AU  - Singh G
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Bag, S
AU  - Bag S
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Sarkar, Mihir
AU  - Sarkar M
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India.
FAU - Chouhan, V S
AU  - Chouhan VS
AD  - Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 
      Bareilly, Uttar Pradesh, 243122, India. Electronic address: 
      vikrant.chouhan@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20191129
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Animals
MH  - Buffaloes/*physiology
MH  - Female
MH  - Fibroblast Growth Factors/genetics/*metabolism
MH  - Gene Expression Regulation/*physiology
MH  - Neovascularization, Physiologic
MH  - Placenta/blood supply/*metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal/physiology
OTO - NOTNLM
OT  - Caruncle
OT  - Cotyledon
OT  - FGF
OT  - Placenta
EDAT- 2019/12/22 06:00
MHDA- 2020/11/11 06:00
CRDT- 2019/12/22 06:00
PHST- 2019/02/08 00:00 [received]
PHST- 2019/11/26 00:00 [revised]
PHST- 2019/11/28 00:00 [accepted]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/12/22 06:00 [entrez]
AID - S0093-691X(19)30533-3 [pii]
AID - 10.1016/j.theriogenology.2019.11.034 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Feb;143:98-112. doi: 10.1016/j.theriogenology.2019.11.034. 
      Epub 2019 Nov 29.