PMID- 31864017
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20220531
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 231
DP  - 2020 Feb
TI  - miR-381-abundant small extracellular vesicles derived from 
      kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs 
      by targeting TAOK1.
PG  - 119682
LID - S0142-9612(19)30781-1 [pii]
LID - 10.1016/j.biomaterials.2019.119682 [doi]
AB  - Small extracellular vesicles (sEVs) derived from mesenchymal stem cells have been 
      shown to possess potent regenerative potential. In this study, we evaluated the 
      chondrogenic effect of sEVs derived from kartogenin-preconditioned human 
      umbilical cord mesenchymal stem cells (hUCMSCs). sEVs were isolated from the 
      supernatants of KGN-preconditioned hUCMSCs (KGN-sEV) by gradient 
      ultra-centrifugation, and internalized by native hUCMSCs, thereby inducing the 
      chondrogenic differentiation. The underlying mechanism of KGN-sEV-induced 
      chondrogenesis was explored by high-throughput sequencing and verified by 
      transfection with the corresponding mimic and inhibitor. Sequencing identified 
      the unique enrichment of a set of miRNAs in KGN-sEV compared with sEVs derived 
      from unpreconditioned cells (un-sEV). Overexpression/inhibition in vitro and in 
      vivo demonstrated that this chondrogenesis-inducing potential was primarily 
      attributed to miR-381-3p, one of the most abundant miRNAs in KGN-sEV. 
      Dual-luciferase reporter assays showed that miR-381-3p promoted chondrogenesis 
      through direct suppression of TAOK1 by targeting its 3' untranslated region, 
      thereby suppressing the Hippo signaling pathway. Collectively, our results 
      highlight the regenerative potential of KGN-sEV to induce chondrogenic 
      differentiation of MSCs, which is mainly achieved by delivering sEV-miR-381-3p, 
      which targets TAOK1.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Jing, Hui
AU  - Jing H
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of 
      Thoracic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Zhang, Xiaoyang
AU  - Zhang X
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Luo, Kai
AU  - Luo K
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Luo, Qiancheng
AU  - Luo Q
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Yin, Meng
AU  - Yin M
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Zhu, Zhongqun
AU  - Zhu Z
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China.
FAU - Zheng, Jinghao
AU  - Zheng J
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic 
      address: zhengjh210@163.com.
FAU - He, Xiaomin
AU  - He X
AD  - Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic 
      address: mrxmhe@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191214
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Anilides)
RN  - 0 (MIRN381 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Phthalic Acids)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TAO1 protein kinase)
RN  - Q93BBN11CP (kartogenin)
SB  - IM
MH  - Anilides
MH  - Cell Differentiation
MH  - *Chondrogenesis
MH  - *Extracellular Vesicles
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs/genetics
MH  - Phthalic Acids
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - Signal Transduction
OTO - NOTNLM
OT  - Chondrogenesis
OT  - Kartogenin
OT  - Mesenchymal stem cells
OT  - Small extracellular vesicles
OT  - miRNA
EDAT- 2019/12/22 06:00
MHDA- 2021/05/15 06:00
CRDT- 2019/12/22 06:00
PHST- 2019/07/08 00:00 [received]
PHST- 2019/12/08 00:00 [revised]
PHST- 2019/12/11 00:00 [accepted]
PHST- 2019/12/22 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2019/12/22 06:00 [entrez]
AID - S0142-9612(19)30781-1 [pii]
AID - 10.1016/j.biomaterials.2019.119682 [doi]
PST - ppublish
SO  - Biomaterials. 2020 Feb;231:119682. doi: 10.1016/j.biomaterials.2019.119682. Epub 
      2019 Dec 14.