PMID- 31866049
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210829
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 233
DP  - 2020 Mar
TI  - Proteomic analysis of decellularized pancreatic matrix identifies collagen V as a 
      critical regulator for islet organogenesis from human pluripotent stem cells.
PG  - 119673
LID - S0142-9612(19)30772-0 [pii]
LID - 10.1016/j.biomaterials.2019.119673 [doi]
AB  - In pancreatic tissue engineering, generating human pancreatic islet organoids 
      from stem cells has been challenging due mainly to a poor understanding of niches 
      required for multicellular tissue self-assembly in vitro. In this study, we aimed 
      to identify bioactive, chemically defined niches from natural, biological 
      materials for islet development in vitro. We investigated the proteomics of 
      decellularized rat pancreatic extracellular matrix (dpECM) hydrogel using 
      advanced bioinformatics analysis, and identified that type V collagen (ColV) is 
      constantly and abundantly present in dpECM hydrogel. Niches provided to human 
      pluripotent stem cells (iPSCs) by presenting ColV in matrix coating substrates 
      permitted stem cells progression into islet-like organoids that consist of all 
      major pancreatic endocrine cell types, i.e. alpha, beta, delta, and pancreatic polypeptide 
      cells. In the presence of ColV niches, gene expressions of all key pancreatic 
      transcription factors and major hormone genes significantly increased in 
      iPSC-derived organoids. Most importantly, ColV-containing microenvironment 
      resulted in enhanced glucose responsive secretions of both insulin and glucagon 
      hormone from organoids. The study demonstrates that ColV is a critical regulator 
      that augments islet self-assembly from iPSCs, and it is feasible to utilize 
      natural biomaterials to build tissue cues essential for multicellular tissue 
      production in vitro.
CI  - Copyright (c) 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Bi, Huanjing
AU  - Bi H
AD  - Department of Biomedical Engineering, Binghamton University, State University of 
      New York (SUNY), Binghamton, NY, 13902, USA.
FAU - Ye, Kaiming
AU  - Ye K
AD  - Department of Biomedical Engineering, Binghamton University, State University of 
      New York (SUNY), Binghamton, NY, 13902, USA; Center of Biomanufacturing for 
      Regenerative Medicine, Binghamton University, State University of New York 
      (SUNY), Binghamton, NY, 13902, USA.
FAU - Jin, Sha
AU  - Jin S
AD  - Department of Biomedical Engineering, Binghamton University, State University of 
      New York (SUNY), Binghamton, NY, 13902, USA; Center of Biomanufacturing for 
      Regenerative Medicine, Binghamton University, State University of New York 
      (SUNY), Binghamton, NY, 13902, USA. Electronic address: sjin@binghamton.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20191206
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Collagen
MH  - Humans
MH  - Organogenesis
MH  - *Pluripotent Stem Cells
MH  - *Proteomics
MH  - Rats
OTO - NOTNLM
OT  - Decellularized pancreatic extracellular matrix
OT  - Human induced pluripotent stem cell
OT  - Islet organoid
OT  - Matrisome
OT  - Proteomics and bioinformatics
OT  - Type V collagen
EDAT- 2019/12/24 06:00
MHDA- 2021/05/15 06:00
CRDT- 2019/12/24 06:00
PHST- 2019/08/24 00:00 [received]
PHST- 2019/11/24 00:00 [revised]
PHST- 2019/12/05 00:00 [accepted]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2019/12/24 06:00 [entrez]
AID - S0142-9612(19)30772-0 [pii]
AID - 10.1016/j.biomaterials.2019.119673 [doi]
PST - ppublish
SO  - Biomaterials. 2020 Mar;233:119673. doi: 10.1016/j.biomaterials.2019.119673. Epub 
      2019 Dec 6.