PMID- 31866541
OWN - NLM
STAT- MEDLINE
DCOM- 20201118
LR  - 20201118
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 145
DP  - 2020 Feb 15
TI  - Electrospun poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/silk fibroin film is a 
      promising scaffold for bone tissue engineering.
PG  - 173-188
LID - S0141-8130(19)36404-9 [pii]
LID - 10.1016/j.ijbiomac.2019.12.149 [doi]
AB  - Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by 
      microorganisms, under unbalanced growth conditions, as a carbon storage compound. 
      PHAs are composed of various monomers such as 3-hydroxybutyrate (3HB) and 
      3-hydroxyhexanoate (3HHx). Silk fibroin (SF) derived from Bombyx mori cocoons, is 
      a widely studied protein polymer commonly used for biomaterial applications. In 
      this study, non-woven electrospun films comprising a copolymer of 3HB and 3HHx 
      [P(3HB-co-3HHx)], SF and their blends were prepared by electrospinning technique. 
      The growth and osteogenic differentiation of human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs) were studied using different types of 
      fabricated electrospun films. The differentiation study revealed that electrospun 
      P(3HB-co-3HHx)/SF film supports the differentiation of hUC-MSCs into the 
      osteogenic lineage, confirmed by histological analysis using Alizarin Red 
      staining, energy dispersive X-ray (EDX) and quantitative real-time PCR analysis 
      (qPCR). Electrospun P(3HB-co-3HHx)/SF film up-regulated the expression of 
      osteogenic marker genes, alkaline phosphatase (ALP) and osteocalcin (OCN), by 
      1.6-fold and 2.8-fold respectively, after 21 days of osteogenic induction. In 
      conclusion, proliferation and osteogenic differentiation of hUC-MSCs were 
      enhanced through the blending of P(3HB-co-3HHx) and SF. The results from this 
      study suggest that electrospun P(3HB-co-3HHx)/SF film is a promising biomaterial 
      for bone tissue engineering.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Ang, Shaik Ling
AU  - Ang SL
AD  - School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia; 
      USM-RIKEN International Center for Aging Science (URICAS), Universiti Sains 
      Malaysia, Penang, Malaysia.
FAU - Shaharuddin, Bakiah
AU  - Shaharuddin B
AD  - Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang, 
      Malaysia; USM-RIKEN International Center for Aging Science (URICAS), Universiti 
      Sains Malaysia, Penang, Malaysia.
FAU - Chuah, Jo-Ann
AU  - Chuah JA
AD  - RIKEN Center for Sustainable Resource Science, RIKEN, Japan; USM-RIKEN 
      International Center for Aging Science (URICAS), Universiti Sains Malaysia, 
      Penang, Malaysia.
FAU - Sudesh, Kumar
AU  - Sudesh K
AD  - School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia; 
      USM-RIKEN International Center for Aging Science (URICAS), Universiti Sains 
      Malaysia, Penang, Malaysia. Electronic address: ksudesh@usm.my.
LA  - eng
PT  - Journal Article
DEP - 20191220
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Biocompatible Materials)
RN  - 0 (Caproates)
RN  - 0 (Polyesters)
RN  - 0 (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate))
RN  - 10191-24-9 (3-hydroxyhexanoic acid)
RN  - 9007-76-5 (Fibroins)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/*pharmacology
MH  - Animals
MH  - Biocompatible Materials/pharmacology
MH  - Bombyx/metabolism
MH  - Bone and Bones/*drug effects
MH  - Caproates/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Fibroins/*pharmacology
MH  - Mesenchymal Stem Cells/drug effects
MH  - Osteogenesis/drug effects
MH  - Polyesters/pharmacology
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Electrospinning
OT  - Mesenchymal stem cells
OT  - Osteogenesis
OT  - Polyhydroxyalkanoates
OT  - Scaffold
OT  - Silk fibroin
COIS- Declaration of competing interest None.
EDAT- 2019/12/24 06:00
MHDA- 2020/11/20 06:00
CRDT- 2019/12/24 06:00
PHST- 2019/08/12 00:00 [received]
PHST- 2019/10/08 00:00 [revised]
PHST- 2019/12/17 00:00 [accepted]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
PHST- 2019/12/24 06:00 [entrez]
AID - S0141-8130(19)36404-9 [pii]
AID - 10.1016/j.ijbiomac.2019.12.149 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Feb 15;145:173-188. doi: 
      10.1016/j.ijbiomac.2019.12.149. Epub 2019 Dec 20.