PMID- 31867139
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231027
IS  - 2164-2591 (Print)
IS  - 2164-2591 (Electronic)
IS  - 2164-2591 (Linking)
VI  - 8
IP  - 6
DP  - 2019 Nov
TI  - Noninvasive Imaging and Correlative Histology of Cone Photoreceptor Structure in 
      the Pig Retina.
PG  - 38
LID - 10.1167/tvst.8.6.38 [doi]
LID - 38
AB  - PURPOSE: To evaluate different methods of studying cone photoreceptor structure 
      in wild-type (WT) and transgenic pigs carrying the human rhodopsin P23H mutant 
      gene (TgP23H). METHODS: For in vivo imaging, pigs were anesthetized with 
      tiletamine-zolazepam and isoflurane and given lidocaine-bupivacaine retrobulbar 
      injections. Stay sutures and a custom head mount were used to hold and steer the 
      head for adaptive optics scanning light ophthalmoscopy (AOSLO). Six WT and TgP23H 
      littermates were imaged at postnatal day 30 (P30), P90, and P180 with AOSLO and 
      optical coherence tomography (OCT), and two additional sets of littermates were 
      imaged at P3 and P15 with OCT only. AOSLO imaging and correlative differential 
      interference contrast microscopy were performed on a P240 WT pig and on WT and 
      TgP23H littermates at P30 and P180. RESULTS: AOSLO cone density generally 
      underestimates histology density (mean difference +/- SD = 24.8% +/- 21.4%). The 
      intensity of the outer retinal hyperreflective OCT band attributed to 
      photoreceptors is attenuated in TgP23H pigs at all ages. In contrast, AOSLO 
      images show cones that retain inner and outer segments through P180. At retinal 
      locations outside the visual streak, TgP23H pigs show a heterogeneous 
      degenerating cone mosaic by using two criteria: variable contrast on a split 
      detector AOSLO and high reflectivity on a confocal AOSLO. CONCLUSIONS: AOSLO 
      reveals that the cone mosaic is similar to ex vivo histology. Its use as a 
      noninvasive tool will enable observation of morphologic changes that arise in the 
      cone mosaic of TgP23H pigs over time. TRANSLATIONAL RELEVANCE: Pigs are widely 
      used for translational studies, and the ability to noninvasively assess cellular 
      changes in the cone mosaic will facilitate more detailed investigations of new 
      retinal disease models as well as outcomes of potential therapies.
CI  - Copyright 2019 The Authors.
FAU - Huckenpahler, Alison L
AU  - Huckenpahler AL
AD  - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Carroll, Joseph
AU  - Carroll J
AD  - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
AD  - Department of Ophthalmology & Visual Sciences, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Salmon, Alexander E
AU  - Salmon AE
AD  - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Sajdak, Benjamin S
AU  - Sajdak BS
AD  - Department of Cell Biology, Neurobiology & Anatomy, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Mastey, Rebecca R
AU  - Mastey RR
AD  - Department of Ophthalmology & Visual Sciences, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Allen, Kenneth P
AU  - Allen KP
AD  - Biomedical Resource Center, Medical College of Wisconsin, Milwaukee, WI, USA.
AD  - Department of Microbiology & Immunology, Medical College of Wisconsin, Milwaukee, 
      WI, USA.
FAU - Kaplan, Henry J
AU  - Kaplan HJ
AD  - Department of Ophthalmology and Visual Sciences, University of Louisville, 
      Louisville, KY, USA.
FAU - McCall, Maureen A
AU  - McCall MA
AD  - Department of Ophthalmology and Visual Sciences, University of Louisville, 
      Louisville, KY, USA.
AD  - Department of Anatomical Sciences and Neurobiology, University of Louisville, 
      Louisville, KY, USA.
LA  - eng
GR  - R01 EY016060/EY/NEI NIH HHS/United States
GR  - F30 EY027706/EY/NEI NIH HHS/United States
GR  - R01 EY026158/EY/NEI NIH HHS/United States
GR  - T32 GM080202/GM/NIGMS NIH HHS/United States
GR  - T32 EY014537/EY/NEI NIH HHS/United States
GR  - C06 RR016511/RR/NCRR NIH HHS/United States
GR  - P30 EY001931/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20191218
PL  - United States
TA  - Transl Vis Sci Technol
JT  - Translational vision science & technology
JID - 101595919
PMC - PMC6922271
OTO - NOTNLM
OT  - adaptive optics scanning light ophthalmoscopy
OT  - cone photoreceptor
OT  - pig
OT  - retina
EDAT- 2019/12/24 06:00
MHDA- 2019/12/24 06:01
PMCR- 2019/12/18
CRDT- 2019/12/24 06:00
PHST- 2019/04/07 00:00 [received]
PHST- 2019/10/04 00:00 [accepted]
PHST- 2019/12/24 06:00 [entrez]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2019/12/24 06:01 [medline]
PHST- 2019/12/18 00:00 [pmc-release]
AID - TVST-19-1548 [pii]
AID - 10.1167/tvst.8.6.38 [doi]
PST - epublish
SO  - Transl Vis Sci Technol. 2019 Dec 18;8(6):38. doi: 10.1167/tvst.8.6.38. 
      eCollection 2019 Nov.