PMID- 31867478
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 4
IP  - 25
DP  - 2019 Dec 17
TI  - Molecular and Cellular Response of Co-cultured Cells toward Cobalt Chloride 
      (CoCl(2))-Induced Hypoxia.
PG  - 20882-20893
LID - 10.1021/acsomega.9b01474 [doi]
AB  - Cobalt chloride (CoCl(2)) is a well-known hypoxia mimetic mediator that induces 
      hypoxia-like responses. CoCl(2), a mediator confirmed to alleviate 
      hypoxia-inducible factor-1 (HIF-1), has been associated with a variety of hypoxic 
      responses. HIF-1 is the foremost transcriptionfactor that is particularly 
      activated during hypoxia and regulates various genes. Therefore, this study aimed 
      to investigate the cellular and molecular responses of the co-cultured cells 
      under the influence of the CoCl(2)-induced hypoxic condition. Mono- and 
      co-cultured C2C12 and 3T3-L1 cells were exposed to CoCl(2), and a significant 
      induction in HIF-1, reactive oxygen species and lipid peroxidase and a reduction 
      in glutathione and catalase were observed. The expressions of proapoptotic genes 
      like Bax, p53, caspase-9, and caspase-3 were notably increased, whereas the 
      antiapoptotic gene, i.e., Bcl2, was downregulated during hypoxia in mono- as well 
      as co-cultured C2C12 cells. However, the co-cultured C2C12 cells show 
      significantly lower induction in oxidative stress and expression of apoptotic 
      genes in comparison to monocultured C2C12 cells. Whereas, the co-cultured 3T3-L1 
      cells show comparatively higher oxidative stress and apoptotic event in 
      comparison to monocultured 3T3-L1 cells. The reason may be the communication 
      between the cells and some soluble factors that help in cell survival/death from 
      hypoxia. Moreover, it may also be due to the fact that fat and muscle cells 
      interact and communicate via proximity and mutual ability when growing together. 
      Therefore, the co-culture system provides a unique approach to intercellular 
      communication between the two different cell types.
CI  - Copyright (c) 2019 American Chemical Society.
FAU - Tripathi, Vinay Kumar
AU  - Tripathi VK
AD  - Department of Animal Science and BK21 PLUS Program and Department of Animal 
      Biotechnology, Jeonbuk National University, Jeonju 561-756, Republic of Korea.
FAU - Subramaniyan, Sivakumar Allur
AU  - Subramaniyan SA
AD  - Department of Animal Science and BK21 PLUS Program and Department of Animal 
      Biotechnology, Jeonbuk National University, Jeonju 561-756, Republic of Korea.
FAU - Hwang, Inho
AU  - Hwang I
AD  - Department of Animal Science and BK21 PLUS Program and Department of Animal 
      Biotechnology, Jeonbuk National University, Jeonju 561-756, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20191202
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC6921254
COIS- The authors declare no competing financial interest.
EDAT- 2019/12/24 06:00
MHDA- 2019/12/24 06:01
PMCR- 2019/12/02
CRDT- 2019/12/24 06:00
PHST- 2019/05/21 00:00 [received]
PHST- 2019/11/13 00:00 [accepted]
PHST- 2019/12/24 06:00 [entrez]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2019/12/24 06:01 [medline]
PHST- 2019/12/02 00:00 [pmc-release]
AID - 10.1021/acsomega.9b01474 [doi]
PST - epublish
SO  - ACS Omega. 2019 Dec 2;4(25):20882-20893. doi: 10.1021/acsomega.9b01474. 
      eCollection 2019 Dec 17.