PMID- 31867910
OWN - NLM
STAT- MEDLINE
DCOM- 20200102
LR  - 20200108
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 44
IP  - 12
DP  - 2019 Dec 25
TI  - [Electroacupuncture improves inflammatory reaction and insulin sensitivity in 
      insulin-resistant obese rats].
PG  - 898-905
LID - 10.13702/j.1000-0607.190209 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture (EA) on inflammatory 
      reaction and insulin sensitivity in insulin-resistant obese (OIR) rats. METHODS: 
      Thirteen male Wistar rats were randomly selected as the control group and fed 
      with common diet. The other 39 rats were fed with high-fat diet for 8 weeks to 
      establish OIR model and then randomized into model, EA and sham EA groups. EA (2 
      Hz, 1 mA) was applied to unilateral "Zusanli" (ST36), "Fenglong" (ST40), 
      "Zhongwan" (CV12) and "Guanyuan" (CV4) for 15 min, once every other day for 8 
      weeks, and sham EA was applied to unilateral 4 control spots about 5 mm lateral 
      to the aforementioned 4 acupoints after shallowly inserting acupuncture needles, 
      but without electric current output. After 8 weeks' intervention, the body weight 
      was recorded and the glucose infusion rate (GIR) measured using 
      hyperinsulinemic-euglycemic clamp technique. At the 6(th) week of intervention, 
      glucose contents of intraperitoneal glucose tolerance test (IPGTT) and 
      intraperitoneal insulin tole-rance test (IPITT) were measured. The levels of 
      serum insulin (INS) and inflammatory factors as C-reactive protein (CRP), IL-6 
      and TNF-alpha were measured by using ELISA at the end of the treatment. The 
      expression levels of IL-6, TNF-alpha, monocyte chemoattractant protein-1(MCP-1), 
      IL-10 and IL-1beta proteins and mRNAs in the abdominal adipose tissues were detected 
      by Western blot and quantitative real time-PCR, separately. The CD68 expression 
      (displaying infiltration of macrophages) of adipose tissue was detected using 
      immunohistochemistry. RESULTS: After modeling, the contents of glucose of IPGTT 
      at 30, 60 and 120 min and those of IPITT at 15, 30, 60 and 120 min, serum INS, 
      CRP, IL-6 and TNF-alpha, as well as the expression levels of IL-6, TNF-alpha, IL-1beta and 
      MCP-1 proteins and mRNAs and CD68 protein were significantly increased (P<0.01, 
      P<0.05), while the levels of GIR and IL-10 protein and mRNA were obviously 
      decreased in the model group in comparison with those of the control group 
      (P<0.01), suggesting an increase of inflammation and a decline of INS 
      sensitivity. Following the interventions, the increased contents of glucose of 
      IPGTT and IPITT, serum INS, CRP, IL-6 and TNF-alpha, expression levels of IL-6, 
      TNF-alpha, IL-1beta and MCP-1 proteins and mRNAs and CD68 protein, and the decreased 
      levels of GIR and IL-10 protein and mRNA were evidently reversed in the EA group 
      compared with the model group (P<0.01, P<0.05) rather than those in the sham EA 
      group (P>0.05). CONCLUSION: EA can reduce the level of inflammation and improve 
      insulin sensitivity in OIR rats.
FAU - Huang, Qi
AU  - Huang Q
AD  - College of Acupuncture-moxibustion and Orthopedics-traumatology, Hubei University 
      of Chinese Medicine, Wuhan 430061, China; Department of Rehabilitation, Central 
      Hospital of Wuhan, Tongji Medical College of Huazhong University of Science and 
      Technology, Wuhan 430014.
FAU - Song, Yan-Juan
AU  - Song YJ
AD  - College of Acupuncture-moxibustion and Orthopedics-traumatology, Hubei University 
      of Chinese Medicine, Wuhan 430061, China.
FAU - Yu, Zhao-Min
AU  - Yu ZM
AD  - Department of Integrated Traditional Chinese and Western Medicine, Union 
      Hospital, Tongji Medical College of Huazhong University of Science and 
      Technology, Wuhan 430022.
FAU - Ren, Jia-Feng
AU  - Ren JF
AD  - College of Acupuncture-moxibustion and Orthopedics-traumatology, Hubei University 
      of Chinese Medicine, Wuhan 430061, China.
FAU - Liang, Feng-Xia
AU  - Liang FX
AD  - College of Acupuncture-moxibustion and Orthopedics-traumatology, Hubei University 
      of Chinese Medicine, Wuhan 430061, China.
FAU - Chen, Rui
AU  - Chen R
AD  - Department of Integrated Traditional Chinese and Western Medicine, Union 
      Hospital, Tongji Medical College of Huazhong University of Science and 
      Technology, Wuhan 430022.
FAU - Xu, Fen
AU  - Xu F
AD  - Department of Rehabilitation, Central Hospital of Wuhan, Tongji Medical College 
      of Huazhong University of Science and Technology, Wuhan 430014.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Insulin)
SB  - IM
MH  - Acupuncture Points
MH  - Animals
MH  - *Electroacupuncture
MH  - Inflammation
MH  - Insulin
MH  - *Insulin Resistance
MH  - Male
MH  - Obesity
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Acupuncture
OT  - Adipose tissue
OT  - Inflammatory reaction
OT  - Insulin resistance
OT  - Obesity
EDAT- 2019/12/24 06:00
MHDA- 2020/01/03 06:00
CRDT- 2019/12/24 06:00
PHST- 2019/12/24 06:00 [entrez]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2020/01/03 06:00 [medline]
AID - 10.13702/j.1000-0607.190209 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2019 Dec 25;44(12):898-905. doi: 10.13702/j.1000-0607.190209.