PMID- 31868949
OWN - NLM
STAT- MEDLINE
DCOM- 20201119
LR  - 20201119
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 44
IP  - 4
DP  - 2020 Apr
TI  - NEAT1/miR-193a-3p/SOX5 axis regulates cartilage matrix degradation in human 
      osteoarthritis.
PG  - 947-957
LID - 10.1002/cbin.11291 [doi]
AB  - Long non-coding RNAs (lncRNAs) were reported to be involved in the progression of 
      osteoarthritis (OA). The aim of this work was to explore the functional role of 
      lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in OA. 
      Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was 
      employed to analyze the expression of microRNA (miR-193a)-3p, NEAT1, and 
      sex-determining region Y-box protein 5 (SOX5), as well as the levels of 
      pro-inflammatory cytokines interleukin-6 (IL-6), IL-1beta, tumor necrosis factor-alpha 
      (TNF-alpha), and IL-8 in OA cartilage tissue and chondrocytes. In addition, flow 
      cytometry was used to measure the apoptosis of chondrocytes. The protein levels 
      of extracellular matrix ACAN, collagen type II alpha1 chain (Col2a1), matrix 
      metalloproteinase-3 (MMP-3), MMP-13, a disintegrin, and metalloproteinase with 
      thrombospondin motifs (ADAMTS)-5 and SOX5 were determined using western blot 
      analysis. Dual-luciferase reporter assay was performed to determine the target 
      relationship among NEAT1, miR-193a-3p, and SOX5. We found that miR-193a-3p 
      expression was downregulated, while NEAT1 and SOX5 were upregulated in OA 
      cartilage tissue and chondrocytes. Both upregulation of miR-193a-3p and knockdown 
      of NEAT1 suppressed inflammation, apoptosis, and reduced the protein levels of 
      MMP-3, MMP-13, and ADAMTS-5, while elevating ACAN and Col2a1 expression in 
      chondrocytes. NEAT1 targeted miR-193a-3p, and SOX5 was targeted by miR-193a-3p. 
      Silencing of miR-193a-3p reversed the NEAT1 knockdown-mediated effect on the 
      inflammation, apoptosis, and production of the extracellular matrix. The 
      introduction of SOX5 abolished the impact of the upregulation of miR-193a-3p on 
      inflammation, apoptosis, and production of extracellular matrix in chondrocytes. 
      In conclusion, NEAT1/miR-193a-3p/SOX5 axis regulates cartilage matrix degradation 
      in human OA.
CI  - (c) 2019 International Federation for Cell Biology.
FAU - Liu, Feng
AU  - Liu F
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
FAU - Liu, Xiangyang
AU  - Liu X
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
FAU - Yang, Yue
AU  - Yang Y
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
FAU - Sun, Zhibo
AU  - Sun Z
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
FAU - Deng, Shuang
AU  - Deng S
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
FAU - Jiang, Zhongping
AU  - Jiang Z
AD  - Department of Emergency, The Renmin Hospital of Wuhan University, No. 238 Jiefang 
      Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, Hubei, 
      China.
FAU - Li, Wen
AU  - Li W
AD  - Department of Emergency, The Renmin Hospital of Wuhan University, No. 238 Jiefang 
      Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, Hubei, 
      China.
FAU - Wu, Fei
AU  - Wu F
AD  - Department of Orthopedics, The Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, and No. 99 Zhangzhidong Road, Wuchang District, 430061, Wuhan, 
      Hubei, China.
LA  - eng
PT  - Journal Article
DEP - 20200107
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (MIRN193 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEAT1 long non-coding RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SOX5 protein, human)
RN  - 0 (SOXD Transcription Factors)
SB  - IM
MH  - Apoptosis
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Cells, Cultured
MH  - Chondrocytes/*metabolism/pathology
MH  - Extracellular Matrix/metabolism
MH  - Humans
MH  - Inflammation/metabolism
MH  - MicroRNAs/*metabolism
MH  - Osteoarthritis, Knee/metabolism/*pathology
MH  - RNA, Long Noncoding/*metabolism
MH  - SOXD Transcription Factors/*metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - NEAT1
OT  - SOX5
OT  - chondrocytes
OT  - miR-193a-3p
OT  - osteoarthritis
EDAT- 2019/12/24 06:00
MHDA- 2020/11/20 06:00
CRDT- 2019/12/24 06:00
PHST- 2019/06/15 00:00 [received]
PHST- 2019/12/21 00:00 [accepted]
PHST- 2019/12/24 06:00 [pubmed]
PHST- 2020/11/20 06:00 [medline]
PHST- 2019/12/24 06:00 [entrez]
AID - 10.1002/cbin.11291 [doi]
PST - ppublish
SO  - Cell Biol Int. 2020 Apr;44(4):947-957. doi: 10.1002/cbin.11291. Epub 2020 Jan 7.