PMID- 31871958
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231225
IS  - 2329-0501 (Print)
IS  - 2329-0501 (Electronic)
IS  - 2329-0501 (Linking)
VI  - 16
DP  - 2020 Mar 13
TI  - Adipose Mesenchymal Cells-Derived EVs Alleviate DOCA-Salt-Induced Hypertension by 
      Promoting Cardio-Renal Protection.
PG  - 63-77
LID - 10.1016/j.omtm.2019.11.002 [doi]
AB  - Hypertension is a long-term condition that can increase organ susceptibility to 
      insults and lead to severe complications such as chronic kidney disease (CKD). 
      Extracellular vesicles (EVs) are cell-derived membrane structures that 
      participate in cell-cell communication by exporting encapsulated molecules to 
      target cells, regulating physiological and pathological processes. We here 
      demonstrate that multiple administration of EVs from adipose-derived mesenchymal 
      stromal cells (ASC-EVs) in deoxycorticosterone acetate (DOCA)-salt hypertensive 
      model can protect renal tissue by maintaining its filtration capacity. Indeed, 
      ASC-EVs downregulated the pro-inflammatory molecules monocyte chemoattracting 
      protein-1 (MCP-1) and plasminogen activating inhibitor-1 (PAI1) and reduced 
      recruitment of macrophages in the kidney. Moreover, ASC-EVs prevented cardiac 
      tissue fibrosis and maintained blood pressure within normal levels, thus 
      demonstrating their multiple favorable effects in different organs. By applying 
      microRNA (miRNA) microarray profile of the kidney of DOCA-salt rats, we 
      identified a selective miRNA signature associated with epithelial-mesenchymal 
      transition (EMT). One of the key pathways found was the axis miR-200-TGF-beta, that 
      was significantly altered by EV administration, thereby affecting the EMT 
      signaling and preventing renal inflammatory response and fibrosis development. 
      Our results indicate that EVs can be a potent therapeutic tool for the treatment 
      of hypertension-induced CKD in cardio-renal syndrome.
CI  - (c) 2019 The Authors.
FAU - Lindoso, Rafael Soares
AU  - Lindoso RS
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Center for Structural Biology and Bioimaging/CENABIO, Federal University 
      of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Institute of Science and Technology for Regenerative Medicine-REGENERA, 
      Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
FAU - Lopes, Jarlene Alecia
AU  - Lopes JA
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Center for Structural Biology and Bioimaging/CENABIO, Federal University 
      of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
FAU - Binato, Renata
AU  - Binato R
AD  - Brazilian National Institute of Cancer, 20230-130 Rio de Janeiro, Brazil.
FAU - Abdelhay, Eliana
AU  - Abdelhay E
AD  - Brazilian National Institute of Cancer, 20230-130 Rio de Janeiro, Brazil.
FAU - Takiya, Christina Maeda
AU  - Takiya CM
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
FAU - Miranda, Kildare Rocha de
AU  - Miranda KR
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Center for Structural Biology and Bioimaging/CENABIO, Federal University 
      of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
FAU - Lara, Lucienne Silva
AU  - Lara LS
AD  - Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 21941-590 
      Rio de Janeiro, Brazil.
FAU - Viola, Antonella
AU  - Viola A
AD  - Department of Biomedical Sciences and Pediatric Research Institute "Citta della 
      Speranza," University of Padova, 35131 Padua, Italy.
FAU - Bussolati, Benedetta
AU  - Bussolati B
AD  - Department of Molecular Biotechnology and Health Sciences, University of Torino, 
      10126 Turin, Italy.
FAU - Vieyra, Adalberto
AU  - Vieyra A
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Center for Structural Biology and Bioimaging/CENABIO, Federal University 
      of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Institute of Science and Technology for Regenerative Medicine-REGENERA, 
      Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - Graduate Program of Translational Biomedicine/BIOTRANS, Grande Rio University, 
      25071-202 Duque de Caxias, Brazil.
FAU - Collino, Federica
AU  - Collino F
AD  - Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de 
      Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - National Institute of Science and Technology for Regenerative Medicine-REGENERA, 
      Federal University of Rio de Janeiro, 21941-902 Rio de Janeiro, Brazil.
AD  - Department of Biomedical Sciences and Pediatric Research Institute "Citta della 
      Speranza," University of Padova, 35131 Padua, Italy.
LA  - eng
PT  - Journal Article
DEP - 20191115
PL  - United States
TA  - Mol Ther Methods Clin Dev
JT  - Molecular therapy. Methods & clinical development
JID - 101624857
PMC - PMC6909095
OTO - NOTNLM
OT  - cardio-renal syndrome
OT  - chronic kidney disease
OT  - epithelial-mesenchymal transition
OT  - extracellular vesicles
OT  - hypertension
OT  - microRNAs
EDAT- 2019/12/25 06:00
MHDA- 2019/12/25 06:01
PMCR- 2019/11/15
CRDT- 2019/12/25 06:00
PHST- 2019/09/10 00:00 [received]
PHST- 2019/11/02 00:00 [accepted]
PHST- 2019/12/25 06:00 [entrez]
PHST- 2019/12/25 06:00 [pubmed]
PHST- 2019/12/25 06:01 [medline]
PHST- 2019/11/15 00:00 [pmc-release]
AID - S2329-0501(19)30127-5 [pii]
AID - 10.1016/j.omtm.2019.11.002 [doi]
PST - epublish
SO  - Mol Ther Methods Clin Dev. 2019 Nov 15;16:63-77. doi: 10.1016/j.omtm.2019.11.002. 
      eCollection 2020 Mar 13.