PMID- 31872861
OWN - NLM
STAT- MEDLINE
DCOM- 20200505
LR  - 20210208
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 153
IP  - 3
DP  - 2020 Feb 8
TI  - Comparison Between Integrated Genomic DNA/RNA Profiling and Fluorescence In Situ 
      Hybridization in the Detection of MYC, BCL-2, and BCL-6 Gene Rearrangements in 
      Large B-Cell Lymphomas.
PG  - 353-359
LID - 10.1093/ajcp/aqz172 [doi]
AB  - OBJECTIVES: To compare fluorescence in situ hybridization (FISH) and a 
      commercially available sequencing assay for comprehensive genomic profiling (CGP) 
      to determine the best approach to identify gene rearrangements (GRs) in large 
      B-cell lymphomas (LBCLs). METHODS: Comparison of standard-of-care FISH assays 
      (including a two-probe approach for MYC; break-apart and fusion probes) and an 
      integrated genomic DNA/RNA sequencing CGP approach on a set of 69 consecutive 
      LBCL cases. RESULTS: CGP detected GRs, including those involving MYC (1), BCL-2 
      (3), and BCL-6 (3), not detected by FISH. FISH detected non-IgH-MYC (4) and BCL-6 
      (2) GRs that were not detected by CGP. In four instances, standalone CGP or FISH 
      testing would have missed a double-hit lymphoma. CONCLUSIONS: CGP was superior to 
      FISH in the detection of IgH-MYC rearrangements but was inferior for the 
      detection of non-IgH-MYC rearrangements. Our study demonstrates the rationale for 
      development of a customized approach to identify GRs in LBCLs.
CI  - (c) American Society for Clinical Pathology, 2019. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Cassidy, Daniel P
AU  - Cassidy DP
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - Chapman, Jennifer R
AU  - Chapman JR
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - Lopez, Rafael
AU  - Lopez R
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - White, Kyle
AU  - White K
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - Fan, Yao-Shan
AU  - Fan YS
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - Casas, Carmen
AU  - Casas C
AD  - Division of Hematopathology, Department of Pathology and Laboratory Medicine, 
      University of Miami, Sylvester Comprehensive Cancer Center, and Jackson Memorial 
      Hospitals, Miami, FL.
FAU - Severson, Eric A
AU  - Severson EA
AD  - Foundation Medicine, Morrisville, NC.
FAU - Vega, Francisco
AU  - Vega F
AD  - Department of Hematopathology, MD Anderson Cancer Center, Houston, TX.
LA  - eng
GR  - R01 CA222918/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (BCL2 protein, human)
RN  - 0 (BCL6 protein, human)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - *Gene Rearrangement
MH  - Genetic Profile
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Large B-Cell, Diffuse/*diagnosis/genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - Proto-Oncogene Proteins c-bcl-6/*genetics
MH  - Proto-Oncogene Proteins c-myc/*genetics
PMC - PMC7007318
OTO - NOTNLM
OT  - MYC
OT  - Comprehensive genomic profiling
OT  - FISH
OT  - Gene rearrangements
OT  - High-grade B-cell lymphoma
OT  - Prognosis
OT  - Sequencing
EDAT- 2019/12/25 06:00
MHDA- 2020/05/06 06:00
PMCR- 2021/02/08
CRDT- 2019/12/25 06:00
PHST- 2019/12/25 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
PHST- 2019/12/25 06:00 [entrez]
PHST- 2021/02/08 00:00 [pmc-release]
AID - 5686236 [pii]
AID - aqz172 [pii]
AID - 10.1093/ajcp/aqz172 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2020 Feb 8;153(3):353-359. doi: 10.1093/ajcp/aqz172.