PMID- 31872875
OWN - NLM
STAT- MEDLINE
DCOM- 20210101
LR  - 20210101
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 130
IP  - 11
DP  - 2020 Nov
TI  - LncRNA miR143HG suppresses miR-21 through methylation to inhibit cell invasion 
      and migration.
PG  - E640-E645
LID - 10.1002/lary.28474 [doi]
AB  - OBJECTIVE: This study aimed to investigate the role of lncRNA miR143HG in 
      laryngeal squamous cell carcinoma (LSCC). METHODS: Quantitative polymerase chain 
      reaction (PCR) and paired t test were used to measure and compare expression 
      levels of miR143HG and miR-21 in LSCC and nontumor tissues. To analyze the 
      interactions between miR143HG and miR-21, UM-SCC-17A cells were transfected 
      miR143HG expression vector or miR-21 mimic. The effects of miR143HG and miR-21 
      overexpression on UM-SCC-17A cell invasion and migration were analyzed by 
      transwell assays. RESULTS: We found that miR143HG was downregulated in LSCC and 
      inversely correlated with miR-21. In LSCC cells, miR143HG overexpression led to 
      the downregulated expression of miR-21, whereas miR-21 overexpression failed to 
      affect miR143HG. Methylation-specific PCR results showed that miR143HG 
      overexpression led to increased methylation of miR-21. Low expression levels of 
      miR143HG were correlated with poor survival. Overexpression of miR143HG led to 
      decreased, whereas miR-21 overexpression resulted in increased rate of LSCC cell 
      migration and invasion. In addition, miR-21 overexpression led to reduced effects 
      of miR143HG on cell invasion and migration. CONCLUSION: Therefore, miR143HG 
      suppresses miR-21 via methylation to regulate cell behaviors in LSCC. LEVEL OF 
      EVIDENCE: NA Laryngoscope, 130:E640-E645, 2020.
CI  - (c) 2019 The American Laryngological, Rhinological and Otological Society, Inc.
FAU - Xun, Wu
AU  - Xun W
AD  - Department of Maxillofacial Surgery, Guangxi Medical University College of 
      Stomatology, Nanning City, Guangxi Province, People's Republic of China.
FAU - Cen, Wei
AU  - Cen W
AUID- ORCID: 0000-0002-3684-7567
AD  - the ENT & HN Surgery Department, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning City, Guangxi Province, People's Republic of China.
FAU - Dahai, Yu
AU  - Dahai Y
AD  - Department of Stomatology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning City, Guangxi Province, People's Republic of China.
FAU - Huaqing, Wei
AU  - Huaqing W
AD  - Department of Cardiology, Wuming Hospital of Guangxi Medical University, Nanning 
      City, Guangxi Province, People's Republic of China.
FAU - Jiping, Su
AU  - Jiping S
AD  - the ENT & HN Surgery Department, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning City, Guangxi Province, People's Republic of China.
FAU - Mengzhu, Guo
AU  - Mengzhu G
AD  - Department of Stomatology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning City, Guangxi Province, People's Republic of China.
FAU - Ning, Meng
AU  - Ning M
AD  - Department of Maxillofacial Surgery, Guangxi Medical University College of 
      Stomatology, Nanning City, Guangxi Province, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191224
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (MIRN143 microRNA, human)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Migration Inhibition/*genetics
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Methylation
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - RNA, Long Noncoding/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics
OTO - NOTNLM
OT  - laryngeal squamous cell carcinoma
OT  - miR-21
OT  - miR143HG
OT  - survival
EDAT- 2019/12/25 06:00
MHDA- 2021/01/02 06:00
CRDT- 2019/12/25 06:00
PHST- 2019/08/27 00:00 [received]
PHST- 2019/10/28 00:00 [revised]
PHST- 2019/11/25 00:00 [accepted]
PHST- 2019/12/25 06:00 [pubmed]
PHST- 2021/01/02 06:00 [medline]
PHST- 2019/12/25 06:00 [entrez]
AID - 10.1002/lary.28474 [doi]
PST - ppublish
SO  - Laryngoscope. 2020 Nov;130(11):E640-E645. doi: 10.1002/lary.28474. Epub 2019 Dec 
      24.