PMID- 31874197
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200127
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 321
DP  - 2020 Mar 15
TI  - Subchondral bone dysplasia mediates susceptibility to osteoarthritis in female 
      adult offspring rats induced by prenatal caffeine exposure.
PG  - 122-130
LID - S0378-4274(19)30420-5 [pii]
LID - 10.1016/j.toxlet.2019.12.026 [doi]
AB  - Our previous studies confirmed that prenatal caffeine exposure (PCE) could induce 
      susceptibility to osteoarthritis in adult offspring rats due to poor chondrocyte 
      differentiation, but its mechanism remains to be further investigated. This study 
      aimed to explore whether subchondral bone dysplasia mediates susceptibility to 
      osteoarthritis in adult offspring rats induced by PCE. Pregnant Wistar rats were 
      treated with caffeine (120 mg/kg.d) or saline from gestational day (GD) 9 to 20. 
      The female offspring were euthanized to collect femurs at GD20, postnatal week 
      (PW) 6, and PW28 (non-ovariectomy and ovariectomy groups) to detect 
      osteoarthritis-like phenotype, subchondral bone mass, ossification center 
      development, and other evidence. The results showed that PCE increased the Mankin 
      score of pathological articular cartilage, but decreased articular cartilage 
      thickness and subchondral bone mass, which were more obvious after ovariectomy. 
      Meanwhile, the correlation analysis results demonstrated that the Mankin score of 
      articular cartilage was significantly negatively correlated with subchondral bone 
      mass, and the thickness of articular cartilage was significantly positively 
      correlated with subchondral bone mass. Further, the length and area of the 
      primary and secondary ossification centers, the number of osteoblasts, and the 
      related genes' expression of osteogenic differentiation (e.g., Runx2, BSP, ALP, 
      and OCN) were all significantly decreased in the PCE group before and after 
      birth. Taken together, PCE induced susceptibility to osteoarthritis in adult 
      female offspring, which was likely related to the subchondral bone dysplasia and 
      reduction of subchondral bone mass production due to developmental disorder of 
      primary and secondary ossification centers caused by osteoblast differentiation 
      disability before and after birth.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Xie, Xingkui
AU  - Xie X
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Department of Pharmacology, Wuhan University School of Basic 
      Medical Sciences, Wuhan 430071, China.
FAU - Tan, Yang
AU  - Tan Y
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Disease, Wuhan 430071, China.
FAU - Xiao, Hao
AU  - Xiao H
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China.
FAU - Wen, Yinxian
AU  - Wen Y
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Disease, Wuhan 430071, China.
FAU - Magdalou, Jacques
AU  - Magdalou J
AD  - UMR 7561 CNRS-Universitede Lorraine, Faculte de Medicine, Vandoeuvre-les-Nancy, 
      France.
FAU - Chen, Liaobin
AU  - Chen L
AD  - Department of Orthopedic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 
      430071, China; Hubei Provincial Key Laboratory of Developmentally Originated 
      Disease, Wuhan 430071, China. Electronic address: lbchen@whu.edu.cn.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally 
      Originated Disease, Wuhan 430071, China. Electronic address: 
      wanghui19@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191223
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Central Nervous System Stimulants)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Bone Diseases, Developmental/*chemically induced/genetics/metabolism/pathology
MH  - Caffeine/*toxicity
MH  - Cartilage, Articular/*drug effects/pathology
MH  - Cell Differentiation/drug effects
MH  - Central Nervous System Stimulants/*toxicity
MH  - Female
MH  - Femur/drug effects/metabolism/pathology
MH  - Gestational Age
MH  - Osteoarthritis/*chemically induced/genetics/metabolism/pathology
MH  - Osteoblasts/drug effects/metabolism/pathology
MH  - Osteogenesis/*drug effects/genetics
MH  - Ovariectomy
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats, Wistar
MH  - Sex Factors
MH  - Tibia/drug effects/metabolism/pathology
OTO - NOTNLM
OT  - Caffeine
OT  - Dysplasia
OT  - Osteoarthritis
OT  - Subchondral bone
EDAT- 2019/12/25 06:00
MHDA- 2020/01/28 06:00
CRDT- 2019/12/25 06:00
PHST- 2019/10/18 00:00 [received]
PHST- 2019/12/17 00:00 [revised]
PHST- 2019/12/18 00:00 [accepted]
PHST- 2019/12/25 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
PHST- 2019/12/25 06:00 [entrez]
AID - S0378-4274(19)30420-5 [pii]
AID - 10.1016/j.toxlet.2019.12.026 [doi]
PST - ppublish
SO  - Toxicol Lett. 2020 Mar 15;321:122-130. doi: 10.1016/j.toxlet.2019.12.026. Epub 
      2019 Dec 23.