PMID- 31877329
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20201109
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 140
DP  - 2020 Mar
TI  - Molecular characterization of Enterococcus faecalis isolates from urinary tract 
      infection and interaction between Enterococcus faecalis encountered Dendritic and 
      Natural Killer cells.
PG  - 103944
LID - S0882-4010(19)31288-4 [pii]
LID - 10.1016/j.micpath.2019.103944 [doi]
AB  - PURPOSE: Enterococcus faecalis is an emerging nosocomial pathogen. The study 
      investigates the E. faecalis specific innate immune cells interplay between 
      Natural Killer cells (NK) and Dendritic cells (DCs) in vitro. The present study 
      also determines the prevalence, phenotype, and genotype of Enterococcus faecalis 
      isolated from paediatric patients with urinary tract infection. MATERIALS AND 
      METHODS: A total of 14 clinical isolates of Enterococcus spp were characterized 
      using standard phenotypic tests and virulence factors were determined by 
      polymerase chain reaction (PCR). Immature monocyte-derived DCs were cultured in 
      the presence of six pathogenic E. faecalis isolates infected DCs were co-cultured 
      with NK cells. Bacteria induced matured DCs and activated NK cells were evaluated 
      by polychromatic flow cytometry. RESULTS: Out of 14 isolates, 13 were identified 
      as E. faecalis. E. faecalis infected DCs differentiated into inflammatory and 
      CD141 + DCs that promote NK cell activation. Activated NK cells significantly 
      elevated the secretion of cytokines and chemokines in infected DCs during E. 
      faecalis. This suggests that DC induced NK cell activation is effectively 
      enhanced by the presence of E. faecalis. CONCLUSIONS: Studies on virulence 
      determinants are necessary to understand the pathogenesis of E. faecalis. DC/NK 
      cross-talk is of particular importance at mucosal surfaces such as the intestine, 
      urinary tract where the immune system exists in intimate association with 
      commensal bacteria. We found E. faecalis specific NK cells activation by infected 
      DC-derived effector signals may involve in the killing of transformed or infected 
      cells, thus coordinating innate and adaptive immune responses. E. faecalis 
      specific DC/NK interaction is necessary for DC maturation and modulation of 
      innate effector functions. Similarly, activated NK cells that induce- maturation 
      of DC by pattern recognition receptors are also required for the generation of 
      bacterial specific adaptive immunity.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Kathirvel, Sujitha
AU  - Kathirvel S
AD  - Cell Signaling Lab, Department of Microbiology, School of Life Sciences, 
      Pondicherry University, Puducherry, 605014, India. Electronic address: 
      sujithakathirvel@gmail.com.
FAU - Mani, Maheswaran
AU  - Mani M
AD  - Cell Signaling Lab, Department of Microbiology, School of Life Sciences, 
      Pondicherry University, Puducherry, 605014, India.
FAU - Gopala Krishnan, Gopi Krishnan
AU  - Gopala Krishnan GK
AD  - Cell Signaling Lab, Department of Microbiology, School of Life Sciences, 
      Pondicherry University, Puducherry, 605014, India.
FAU - Sethumadhavan, Aiswarya
AU  - Sethumadhavan A
AD  - Cell Signaling Lab, Department of Microbiology, School of Life Sciences, 
      Pondicherry University, Puducherry, 605014, India.
FAU - Vijayalakshmi, T
AU  - Vijayalakshmi T
AD  - Department of Microbiology, Karpaga Vinayaga Institute of Medical Sciences and 
      Research Institute, Chennai, India.
FAU - Ponnan, Sivasankaran Munusamy
AU  - Ponnan SM
AD  - Department of HIV, National Institute for Research in Tuberculosis (Indian 
      Council of Medical Research), Chennai, India.
FAU - Hanna, Luke Elizabeth
AU  - Hanna LE
AD  - Department of HIV, National Institute for Research in Tuberculosis (Indian 
      Council of Medical Research), Chennai, India.
FAU - Mathaiyan, Manikannan
AU  - Mathaiyan M
AD  - Department of HIV, National Institute for Research in Tuberculosis (Indian 
      Council of Medical Research), Chennai, India.
LA  - eng
PT  - Journal Article
DEP - 20191223
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Cytokines)
SB  - IM
MH  - Adaptive Immunity
MH  - *Cell Communication
MH  - Cross Infection/microbiology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - *Enterococcus faecalis/genetics/immunology/metabolism
MH  - Flow Cytometry
MH  - Genes, Bacterial
MH  - Humans
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Urinary Tract Infections/immunology/*microbiology
MH  - Virulence/genetics
OTO - NOTNLM
OT  - Antimicrobial resistance
OT  - DC
OT  - Enterococcus faecalis
OT  - Gelatinase
OT  - NK cells
OT  - UTI
OT  - Virulence
COIS- Declaration of competing interest None.
EDAT- 2019/12/27 06:00
MHDA- 2020/11/11 06:00
CRDT- 2019/12/27 06:00
PHST- 2019/07/19 00:00 [received]
PHST- 2019/11/26 00:00 [revised]
PHST- 2019/12/20 00:00 [accepted]
PHST- 2019/12/27 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/12/27 06:00 [entrez]
AID - S0882-4010(19)31288-4 [pii]
AID - 10.1016/j.micpath.2019.103944 [doi]
PST - ppublish
SO  - Microb Pathog. 2020 Mar;140:103944. doi: 10.1016/j.micpath.2019.103944. Epub 2019 
      Dec 23.