PMID- 31877847
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Dec 22
TI  - Modification of Tumor Necrosis Factor-alpha and C-C Motif Chemokine Ligand 18 
      Secretion by Monocytes Derived from Patients with Diabetic Foot Syndrome.
LID - 10.3390/biology9010003 [doi]
LID - 3
AB  - Background: This study involves the investigation of spontaneous and induced 
      secretion of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) and 
      the anti-inflammatory chemokine C-C motif chemokine ligand 18 (CCL18) by 
      monocytes isolated from blood of patients with long-term type 2 diabetes mellitus 
      (T2DM), both with or without foot ulcers. Methods: A total of 121 patients with 
      T2DM (79 without diabetic foot syndrome (DFS) and 42 patients with DFS) were 
      included. Cluster of Differentiation 14 (CD14+) monocytes were isolated from 
      patients' blood and stimulated by interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) 
      for induction of pro- and anti-inflammatory monocyte activation, respectively. 
      The concentrations of TNF-alpha and CCL18 in the culture medium were measured using 
      ELISA on day 1 and day 6 after cell stimulation. Results: We found a correlation 
      between glycated hemoglobin (HbA1c) and stimulated secretion levels of TNF-alpha (r = 
      0.726, p = 0.027) and CCL18 (r = -0.949, p = 0.051) in patients with DFS. There 
      was an increase of pro- and anti-inflammatory activation of monocytes in all 
      patients with different durations of DFS (p < 0.05). However, no stimulation of 
      anti-inflammatory activation was detected in patients with DFS lasting more than 
      6 months (p = 0.033). Conclusions: Our study showed an increase in 
      pro-inflammatory secretion and a decrease in anti-inflammatory secretion by 
      monocytes isolated from blood of patients with T2DM depending on HbA1c levels and 
      duration of the inflammatory process. These findings allow us to assume that 
      monocytes isolated from T2DM patients are characterized by a biased ability to 
      respond towards pro-inflammatory stimulation, contributing to the chronic wound 
      process.
FAU - Galstyan, Karine O
AU  - Galstyan KO
AD  - Federal State Autonomous Educational Institution of Higher Education I.M. 
      Sechenov First Moscow State Medical University (Sechenov University), Ministry of 
      Health of the Russian Federation, 119991 Moscow, Russia.
AD  - City Clinical Hospital numero sign 67 named after L.A. Vorokhobov, 123423 Moscow, Russia.
FAU - Nedosugova, Ludmila V
AU  - Nedosugova LV
AD  - Federal State Autonomous Educational Institution of Higher Education I.M. 
      Sechenov First Moscow State Medical University (Sechenov University), Ministry of 
      Health of the Russian Federation, 119991 Moscow, Russia.
FAU - Martirosian, Narine S
AU  - Martirosian NS
AUID- ORCID: 0000-0002-0202-1257
AD  - Federal State Autonomous Educational Institution of Higher Education I.M. 
      Sechenov First Moscow State Medical University (Sechenov University), Ministry of 
      Health of the Russian Federation, 119991 Moscow, Russia.
AD  - City Clinical Hospital numero sign 67 named after L.A. Vorokhobov, 123423 Moscow, Russia.
FAU - Nikiforov, Nikita G
AU  - Nikiforov NG
AD  - Laboratory of Medical Genetics, Institute of Experimental Cardiology, National 
      Medical Research Center of Cardiology, 121552 Moscow, Russia.
AD  - Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 
      125315 Moscow, Russia.
AD  - Center of Collective Usage, Institute of Gene Biology, 119991 Moscow, Russia.
FAU - Elizova, Natalia V
AU  - Elizova NV
AD  - Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 
      125315 Moscow, Russia.
FAU - Kolmychkova, Kira I
AU  - Kolmychkova KI
AUID- ORCID: 0000-0002-2953-5901
AD  - Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 
      125315 Moscow, Russia.
FAU - Sobenin, Igor A
AU  - Sobenin IA
AUID- ORCID: 0000-0003-0978-6444
AD  - Laboratory of Medical Genetics, Institute of Experimental Cardiology, National 
      Medical Research Center of Cardiology, 121552 Moscow, Russia.
AD  - Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 
      125315 Moscow, Russia.
AD  - Research Institute of Threpsology and Healthy Longevity, Plekhanov Russian 
      University of Economics, 117997 Moscow, Russia.
FAU - Orekhov, Alexander N
AU  - Orekhov AN
AUID- ORCID: 0000-0002-3318-4681
AD  - Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 
      125315 Moscow, Russia.
LA  - eng
GR  - 19-15-00010/Russian Science Foundation/
PT  - Journal Article
DEP - 20191222
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC7168305
OTO - NOTNLM
OT  - C-C motif chemokine ligand 18
OT  - anti-inflammatory
OT  - chemokine
OT  - cytokine
OT  - diabetic foot syndrome
OT  - monocyte response
OT  - pro-inflammatory
OT  - tumor necrosis factor-alpha
OT  - type 2 diabetes mellitus
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/28 06:00
MHDA- 2019/12/28 06:01
PMCR- 2020/01/01
CRDT- 2019/12/28 06:00
PHST- 2019/11/18 00:00 [received]
PHST- 2019/12/18 00:00 [revised]
PHST- 2019/12/19 00:00 [accepted]
PHST- 2019/12/28 06:00 [entrez]
PHST- 2019/12/28 06:00 [pubmed]
PHST- 2019/12/28 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - biology9010003 [pii]
AID - biology-09-00003 [pii]
AID - 10.3390/biology9010003 [doi]
PST - epublish
SO  - Biology (Basel). 2019 Dec 22;9(1):3. doi: 10.3390/biology9010003.