PMID- 31878833
OWN - NLM
STAT- MEDLINE
DCOM- 20210129
LR  - 20211011
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 19
IP  - 2
DP  - 2020 Jan
TI  - Circular RNA circDENND2A protects H9c2 cells from oxygen glucose 
      deprivation-induced apoptosis through sponging microRNA-34a.
PG  - 246-255
LID - 10.1080/15384101.2019.1708029 [doi]
AB  - Background/Aims: Myocardial ischemia (MI) is a serious threat to human health. 
      Circular RNAs (circRNAs) play an important role in many diseases including MI. 
      The effect and mechanism of circDENND2A in MI have not been studied.Methods: We 
      used oxygen glucose deprivation (OGD) treatment to simulate MI in vitro. We 
      detected circDENND2A and microRNA (miR)-34a levels by RT-qPCR. The transfection 
      process used INTERFER and jetPRIME. Cell growth indexes including viability, 
      apoptosis, and migration were detected by CCK8, flow cytometry, and transwell 
      assays, respectively. In addition, the Bax, Cleaved-Caspase-3, matrix 
      metalloproteinase (MMP)-2, MMP-9 and pathway-related protein levels were tested 
      by Western blot.Results: OGD upregulated circDENND2A expression in H9c2 cells. 
      Overexpression of circDENND2A enhanced cell viability and migration but declined 
      apoptosis under OGD. Silenced circDENND 2A played the opposite effects. 
      circDENND2A negatively regulated miR-34a. miR-34a overexpression weakened the 
      protective effects of circDENND2A in OGD-injury. Moreover, we considered 
      circDENND2A and miR-34a may work via beta-catenin and Ras/Raf/MEK/ERK 
      pathways.Conclusion: circDENND2A overexpression enhanced OGD-inhibited cell 
      viability and migration but declined OGD-promoted apoptosis by downregulating 
      miR-34a and via beta-catenin and Ras/Raf/MEK/ERK pathways.
FAU - Shao, Yuanxia
AU  - Shao Y
AD  - Department of Cardiology, Jining No.1 People's Hospital, Jining, Shandong, China.
FAU - Zhong, Peng
AU  - Zhong P
AD  - Department of Cardiology, Jining No.1 People's Hospital, Jining, Shandong, China.
FAU - Sheng, Li
AU  - Sheng L
AD  - Department of Cardiology, Jining No.1 People's Hospital, Jining, Shandong, China.
FAU - Zheng, Hongjian
AU  - Zheng H
AUID- ORCID: 0000-0002-5208-1880
AD  - Department of Cardiology, Jining No.1 People's Hospital, Jining, Shandong, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20191227
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (MIRN34 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (beta Catenin)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
RIN - Cell Cycle. 2022 Jan;21(1):109. PMID: 34633898
MH  - Aged
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Line
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - *Cytoprotection/genetics
MH  - Down-Regulation/genetics
MH  - Female
MH  - Gene Silencing
MH  - Glucose/*deficiency
MH  - Humans
MH  - MAP Kinase Signaling System
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Myocardial Infarction/genetics
MH  - Oxygen/*metabolism
MH  - RNA, Circular/genetics/*metabolism
MH  - Rats
MH  - Up-Regulation/genetics
MH  - beta Catenin/metabolism
PMC - PMC6961690
OTO - NOTNLM
OT  - Myocardial ischemia
OT  - apoptosis
OT  - circDENND2A
OT  - miR-34a
OT  - migration
OT  - viability
EDAT- 2019/12/28 06:00
MHDA- 2021/01/30 06:00
PMCR- 2020/12/27
CRDT- 2019/12/28 06:00
PHST- 2019/12/28 06:00 [pubmed]
PHST- 2021/01/30 06:00 [medline]
PHST- 2019/12/28 06:00 [entrez]
PHST- 2020/12/27 00:00 [pmc-release]
AID - 1708029 [pii]
AID - 10.1080/15384101.2019.1708029 [doi]
PST - ppublish
SO  - Cell Cycle. 2020 Jan;19(2):246-255. doi: 10.1080/15384101.2019.1708029. Epub 2019 
      Dec 27.