PMID- 31878994
OWN - NLM
STAT- MEDLINE
DCOM- 20200116
LR  - 20211204
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 35
IP  - 11
DP  - 2019 Nov
TI  - [TP53BP2/ASPP2 inhibits autophagy of HepG2 cells by activating mTOR pathway in a 
      p53-independent manner].
PG  - 986-991
AB  - Objective To study the regulative effect of tumor protein p53 binding protein 
      2/p53 apoptosis-stimulating protein 2 (TP53BP2/ASPP2) on the autophagy of HepG2 
      human hepatoma cells and its mechanism. Methods The expression of ASPP2 was 
      up-regulated or down-regulated by HepG2 cells infected with adenovirus and 
      lentivirus. HepG2 cells were cultured in medium without fetal bovine serum for 24 
      hours. Western blotting was used to detect the expression of 
      microtubule-associated protein 1 light chain 3 (LC3), beclin1, P62, 
      autophagy-related gene 5 (ATG5), ATG7, and mammalian target of rapamycin (mTOR) 
      pathway-associated protein mTOR, phospho-mTOR (p-mTOR), eukaryotic transcription 
      initiation factor 4E binding protein 1 (4EBP1), phospho-4EBP1 (p-4EBP1), 
      ribosomal protein S6, phospho-S6 (p-S6), ribosomal S6 kinase B1 (RPS6KB1/p70S6K), 
      phospho-p70S6K (p-p70S6K). Fluorescence microscopy was used to detect the green 
      fluorescent protein-microtubule-associated protein 1 light chain 3 (GFP-LC3) 
      fusion protein expressed by the cells. At the same time, the Hep3B cells without 
      p53 expression were infected with lentivirus, and the levels of ASPP2 in the 
      cells were knocked down. Western blotting was performed to detect the expression 
      of ASPP2 and mTOR pathway-associated protein mTOR, p-mTOR, 4EBP1, p-4EBP1, S6, 
      p-S6, RPS6KB1/p70S6K, p-p70S6K. Results When the expression of ASPP2 was 
      up-regulated, the mTOR complex 1 (mTORC1) pathway was activated, and the 
      expression of autophagy-related proteins and the number of autophagosomes were 
      reduced. After the expression of ASPP2 was down-regulated, the mTORC1 pathway was 
      inhibited, and the expression level of autophagy-related proteins and the number 
      of autophagosomes increased. In Hep3B cells with p53 expression silence, the 
      mTORC1 pathway was still inhibited when ASPP2 wasdown-regulated. Conclusion ASPP2 
      inhibits the autophagy of HepG2 cells by activating mTOR pathway in a 
      p53-independent manner.
FAU - Xu, Ping
AU  - Xu P
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Beijing Engineering 
      Research Center for Precision Medicine and Transformation of Hepatitis and Liver 
      Cancer, Capital Medical University, Beijing 100069, China.
FAU - Shi, Honglin
AU  - Shi H
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Beijing Engineering 
      Research Center for Precision Medicine and Transformation of Hepatitis and Liver 
      Cancer, Capital Medical University, Beijing 100069, China.
FAU - Jiao, Yan
AU  - Jiao Y
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Beijing Engineering 
      Research Center for Precision Medicine and Transformation of Hepatitis and Liver 
      Cancer, Capital Medical University, Beijing 100069, China.
FAU - Chen, Dexi
AU  - Chen D
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Beijing Engineering 
      Research Center for Precision Medicine and Transformation of Hepatitis and Liver 
      Cancer, Capital Medical University, Beijing 100069, China.
FAU - Shi, Hongbo
AU  - Shi H
AD  - Beijing Institute of Hepatology, Beijing Youan Hospital, Beijing Engineering 
      Research Center for Precision Medicine and Transformation of Hepatitis and Liver 
      Cancer, Capital Medical University, Beijing 100069, China. *Corresponding author, 
      E-mail: shb411@126.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (TP53BP2 protein, human)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - *Autophagy
MH  - Hep G2 Cells
MH  - Humans
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Tumor Suppressor Protein p53
EDAT- 2019/12/28 06:00
MHDA- 2020/01/17 06:00
CRDT- 2019/12/28 06:00
PHST- 2019/12/28 06:00 [entrez]
PHST- 2019/12/28 06:00 [pubmed]
PHST- 2020/01/17 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 Nov;35(11):986-991.