PMID- 31882422
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200622
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 49
IP  - 6
DP  - 2019 Nov
TI  - NFATc1 is a Necessary Transcription Factor in Polarisation of T Helper Type 2 
      Lymphocytes Induced by Thymic Stromal Lymphopoietin.
PG  - 722-729
AB  - Thymic stromal lymphopoietin (TSLP) activates lung dendritic cells (DCs) to 
      promote a T helper type 2 lymphocyte (Th2) response in animal models. However, 
      the mechanism behind this process remains unclear. In this study, we investigate 
      the role of a nuclear factor for activated T-cells 1 (NFATc1) in the TSLP-induced 
      polarisation towards a Th2 response. A cluster of differentiated (CD)14(+) 
      peripheral blood mononuclear cells (PBMCs) and naive T cells were isolated from 
      blood collected from healthy human volunteers, and TSLP was used to induce DC 
      maturation. The effects of TSLP-DCs and treatments with FK506, an NFATc1 
      inhibitor, on naive T cell differentiation were monitored by measuring the 
      interleukin (IL)-4, IL-13, and interferon-gamma (IFN-gamma) expression levels. In 
      addition, the mRNA levels of T-box expression in T cells (T-bet), GATA binding 
      protein 3 (GATA-3), TSLP, and NFATc1 were measured for the same purpose. IL-4, 
      IL-13, and mRNA levels of GATA-3 and NFATc1 significantly increased with TSLP-DC 
      induction (P<0.01), indicating polarization towards the Th2 response. These 
      changes were reversed by treatment with FK506 (P<0.01). Our findings suggest that 
      NFATc1 plays a key role in the TSLP-induced differentiation of T cells to Th2, 
      and NFATc1 is a potential therapeutic target for treating allergic diseases.
CI  - (c) 2019 by the Association of Clinical Scientists, Inc.
FAU - Li, Ya-Ting
AU  - Li YT
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Shen, Zhen-Yu
AU  - Shen ZY
AD  - Department of Pediatrics, the First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Ling, Ye-Sheng
AU  - Ling YS
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Duan, Meng-Qi
AU  - Duan MQ
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Ye, Hui-Qing
AU  - Ye HQ
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Yang, Li-Fen
AU  - Yang LF
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Mai, You-Gang
AU  - Mai YG
AD  - Department of Pediatrics, Sun Yat-sen Memorial Hospital, Guangzhou, China 
      chenzhuanggui@126.com.
FAU - Chen, Zhuang-Gui
AU  - Chen ZG
AD  - Department of Pediatrics, the Third Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China chenzhuanggui@126.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (CD14 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (NFATC1 protein, human)
RN  - 0 (TSLP protein, human)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Polarity
MH  - Cells, Cultured
MH  - Cytokines/genetics/*metabolism/pharmacology
MH  - Dendritic Cells
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology/metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - NFATC Transcription Factors/antagonists & inhibitors/genetics/*metabolism
MH  - Tacrolimus/pharmacology
MH  - Th1 Cells/drug effects/metabolism
MH  - Th2 Cells/*cytology/drug effects/metabolism
OTO - NOTNLM
OT  - Dendritic cells
OT  - GATA-3
OT  - T helper type 2 lymphocytes
OT  - nuclear factor of activated T-cells 1
OT  - thymic stromal lymphopoietin
EDAT- 2019/12/29 06:00
MHDA- 2020/06/23 06:00
CRDT- 2019/12/29 06:00
PHST- 2019/12/29 06:00 [entrez]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
AID - 49/6/722 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2019 Nov;49(6):722-729.