PMID- 31882466
OWN - NLM
STAT- MEDLINE
DCOM- 20200602
LR  - 20231113
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 34
IP  - 1
DP  - 2020 Jan-Feb
TI  - Radical-scavenging and Pro-/anti-inflammatory Activity of Tetracycline and 
      Related Phenolic Compounds With or Without Visible Light Irradiation.
PG  - 81-94
LID - 10.21873/invivo.11748 [doi]
AB  - BACKGROUND/AIM: Microbial tetracycline (TC) pastes have been employed to treat 
      oral bacterial infection. In the present study, we investigated the kinetic 
      radical-scavenging and pro-/anti-inflammatory activity of TC with or without 
      visible light irradiation (VLI). MATERIALS AND METHODS: The radical-scavenging 
      activity of TC and minocycline (MC) was determined by differential scanning 
      calorimetry (DSC). The stoichiometric factor (n) and the rate constant of 
      inhibition and propagation (k(inh)/k(p)) were determined. The levels of 
      cyclooxygenase-2 (Cox2), tumor necrosis factor-alpha (Tnfalpha) or nitric oxide synthase 
      2 (Nos2) mRNA in RAW264.7 cells stimulated with lipopolysaccharide (LPS) were 
      investigated using real-time reverse transcriptase-polymerase chain reaction. 
      RESULTS: The n and k(inh)/k(p) values for 1 mM TC in 2,2'-azobisisobutyronitrile 
      and benzoyl peroxide systems were 0.1-0.2 and 119-250, respectively, whereas the 
      corresponding values for quercetin (QU) and resveratrol (RE) were 2-4 and 7-15, 
      respectively. In RAW264.7 cells stimulated with LPS, Cox2 and Tnfalpha mRNA were 
      over-expressed in the presence of TC. MC down-regulated only the expression of 
      Cox2 by about 50% in LPS-stimulated cells. The anti-inflammatory activity 
      determined on the basis of Cox2 inhibition declined in the order QU>RE>MC>TC. 
      Upon application of VLI, only TC down-regulated the expression of LPS-stimulated 
      Cox2 and Tnfalpha mRNA. After exposure to VLI, TC, but not MC, markedly up-regulated 
      hemoxygenase-1 (Ho-1) expression. CONCLUSION: TC is a chain-breaking antioxidant 
      with a large k(inh) Upon activation by VLI, TC may undergo degradation and its 
      degradation products affect pleiotropic mediators such as Cox2, Tnfalpha and Ho-1. TC 
      may be useful as a local photodynamic therapy for periodontal diseases.
CI  - Copyright(c) 2020, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Murakami, Yukio
AU  - Murakami Y
AD  - Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and 
      Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan 
      ymura@dent.meikai.ac.jp.
FAU - Kawata, Akifumi
AU  - Kawata A
AD  - Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and 
      Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan.
FAU - Suzuki, Seiji
AU  - Suzuki S
AD  - Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and 
      Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan.
FAU - Fujisawa, Seiichiro
AU  - Fujisawa S
AD  - Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and 
      Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Cyclooxygenase 2 Inhibitors)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tetracyclines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/metabolism/pharmacology
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/metabolism
MH  - Cell Line
MH  - Cyclooxygenase 2/metabolism
MH  - Cyclooxygenase 2 Inhibitors/pharmacology
MH  - Down-Regulation/drug effects
MH  - Free Radical Scavengers/pharmacology
MH  - Heme Oxygenase-1/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Periodontal Diseases/chemically induced/drug therapy/metabolism
MH  - Phenols/*pharmacology
MH  - Quercetin/pharmacology
MH  - RAW 264.7 Cells
MH  - RNA, Messenger/metabolism
MH  - Resveratrol/pharmacology
MH  - Tetracyclines/*pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation/drug effects
PMC - PMC6984092
OTO - NOTNLM
OT  - Cox2
OT  - Ho-1
OT  - LPS
OT  - Nos2
OT  - RAW264.7 cell
OT  - Radical-scavenging activity
OT  - Tnfalpha
OT  - tetracyclines
OT  - visible light
COIS- The Authors have no conflicts of interest to declare regarding this study.
EDAT- 2019/12/29 06:00
MHDA- 2020/06/03 06:00
PMCR- 2020/01/03
CRDT- 2019/12/29 06:00
PHST- 2019/09/30 00:00 [received]
PHST- 2019/10/17 00:00 [revised]
PHST- 2019/10/21 00:00 [accepted]
PHST- 2019/12/29 06:00 [entrez]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/06/03 06:00 [medline]
PHST- 2020/01/03 00:00 [pmc-release]
AID - 34/1/81 [pii]
AID - 10.21873/invivo.11748 [doi]
PST - ppublish
SO  - In Vivo. 2020 Jan-Feb;34(1):81-94. doi: 10.21873/invivo.11748.