PMID- 31882833
OWN - NLM
STAT- MEDLINE
DCOM- 20201116
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Dec 27
TI  - Differential immune response modulation in early Leishmania amazonensis infection 
      of BALB/c and C57BL/6 macrophages based on transcriptome profiles.
PG  - 19841
LID - 10.1038/s41598-019-56305-1 [doi]
LID - 19841
AB  - The fate of Leishmania infection can be strongly influenced by the host genetic 
      background. In this work, we describe gene expression modulation of the immune 
      system based on dual global transcriptome profiles of bone marrow-derived 
      macrophages (BMDMs) from BALB/c and C57BL/6 mice infected with Leishmania 
      amazonensis. A total of 12,641 host transcripts were identified according to the 
      alignment to the Mus musculus genome. Differentially expressed genes (DEGs) 
      profiling revealed a differential modulation of the basal genetic background 
      between the two hosts independent of L. amazonensis infection. In addition, in 
      response to early L. amazonensis infection, 10 genes were modulated in infected 
      BALB/c vs. non-infected BALB/c macrophages; and 127 genes were modulated in 
      infected C57BL/6 vs. non-infected C57BL/6 macrophages. These modulated genes 
      appeared to be related to the main immune response processes, such as 
      recognition, antigen presentation, costimulation and proliferation. The distinct 
      gene expression was correlated with the susceptibility and resistance to 
      infection of each host. Furthermore, upon comparing the DEGs in BMDMs vs. 
      peritoneal macrophages, we observed no differences in the gene expression 
      patterns of Jun, Fcgr1 and Il1b, suggesting a similar activation trends of 
      transcription factor binding, recognition and phagocytosis, as well as the 
      proinflammatory cytokine production in response to early L. amazonensis 
      infection. Analysis of the DEG profile of the parasite revealed only one DEG 
      among the 8,282 transcripts, indicating that parasite gene expression in early 
      infection does not depend on the host genetic background.
FAU - Aoki, Juliana Ide
AU  - Aoki JI
AUID- ORCID: 0000-0002-1745-0310
AD  - Department of Physiology, Institute of Bioscience, University of Sao Paulo, Sao 
      Paulo, Brazil. juaoki@usp.br.
FAU - Muxel, Sandra Marcia
AU  - Muxel SM
AD  - Department of Physiology, Institute of Bioscience, University of Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Zampieri, Ricardo Andrade
AU  - Zampieri RA
AD  - Department of Physiology, Institute of Bioscience, University of Sao Paulo, Sao 
      Paulo, Brazil.
FAU - Muller, Karl Erik
AU  - Muller KE
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
AD  - Department of Internal Medicine, Drammen Hospital, Drammen, Norway.
FAU - Nerland, Audun Helge
AU  - Nerland AH
AUID- ORCID: 0000-0002-8272-0276
AD  - Department of Clinical Science, University of Bergen, Bergen, Norway.
FAU - Floeter-Winter, Lucile Maria
AU  - Floeter-Winter LM
AD  - Department of Physiology, Institute of Bioscience, University of Sao Paulo, Sao 
      Paulo, Brazil. lucile@ib.usp.br.
LA  - eng
PT  - Journal Article
DEP - 20191227
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
EIN - Sci Rep. 2020 Mar 4;10(1):4365. PMID: 32127634
MH  - Animals
MH  - Gene Expression Profiling/*methods
MH  - Host-Parasite Interactions
MH  - Leishmania/*immunology/physiology
MH  - Leishmaniasis/genetics/*immunology/parasitology
MH  - Macrophages/*metabolism/parasitology
MH  - Macrophages, Peritoneal/*metabolism/parasitology
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Transcriptome
PMC - PMC6934472
COIS- The authors declare no competing interests.
EDAT- 2019/12/29 06:00
MHDA- 2020/11/18 06:00
PMCR- 2019/12/27
CRDT- 2019/12/29 06:00
PHST- 2019/03/01 00:00 [received]
PHST- 2019/12/10 00:00 [accepted]
PHST- 2019/12/29 06:00 [entrez]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/11/18 06:00 [medline]
PHST- 2019/12/27 00:00 [pmc-release]
AID - 10.1038/s41598-019-56305-1 [pii]
AID - 56305 [pii]
AID - 10.1038/s41598-019-56305-1 [doi]
PST - epublish
SO  - Sci Rep. 2019 Dec 27;9(1):19841. doi: 10.1038/s41598-019-56305-1.