PMID- 31883057 OWN - NLM STAT- MEDLINE DCOM- 20200924 LR - 20200924 IS - 1993-0402 (Electronic) IS - 1672-0415 (Linking) VI - 26 IP - 3 DP - 2020 Mar TI - Qingxuan Jiangya Decoction () Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-beta 1/Smad Pathway in Spontaneously Hypertensive Rats. PG - 180-187 LID - 10.1007/s11655-019-2705-7 [doi] AB - OBJECTIVE: To elevate the effects of Qingxuan Jiangya Decoction (, QXJYD) on hypertension and vascular structural remodeling (VSR) in spontaneously hypertensive rats (SHRs), and investigate the underlying mechanisms. METHODS: SHRs (n=8) were given intra-gastric administration with 60 mg/kg of QXJYD or saline, daily for 8 weeks, while rats in SHR-control (n=8) and WKY (n=8) groups were received equal volumes of saline solution. Systolic blood pressures (SBP), diastolic blood pressures (DBP) and mean blood pressures (MBP) were measured once a week. The levels of angiotensin II (Ang II), endothelin 1 (ET-1) and plasma renin activity (PRA) were tested by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, respectively. The effect of QXJYD on VSR was determined by examining the media thickness and the ex vivo contractility of thoracic aortic. The proliferation and fibrosis of vascular smooth muscle cells (VSMCs) were examined via immunohistochemical (IHC) staining for proliferating cell nuclear antigen (PCNA), collagen I and collagen III, respectively. The mRNA and protein expressions of transforming growth factor beta 1 (TGF-beta 1), Smad3 and phosphorylation of Smad3 in thoracic aorta tissues were determined by real-time polymerase chain reaction (PCR) and Western blot assay, respectively. RESULTS: QXJYD treatment led to a significant decrease of the elevation of blood pressure in SHRs and reduced the levels of Ang II, ET-1 and PRA in the serum (P<0.05). In addition, QXJYD treatment remarkably ameliorated VSR and vascular function in SHRs. Moreover, QXJYD inhibited VSMC proliferation and fibrosis by suppressing the expression of PCNA, collagen I and collagen III in thoracic aortic. Furthermore, QXJYD inhibited the expression of TGF-beta 1, Smad3 and the phosphorylation of Smad3, respectively (P<0.05). CONCLUSION: QXJYD reversed VSR by inhibiting VSMC proliferation and collagen deposition via regulation of TGF-beta 1/Smad signaling pathway, which may, in part, illuminate its anti-hypertensive activities. FAU - He, Fei AU - He F AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Chu, Jian-Feng AU - Chu JF AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, 350122, China. FAU - Chen, Hong-Wei AU - Chen HW AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Lin, Wei AU - Lin W AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, 350122, China. FAU - Lin, Shan AU - Lin S AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. FAU - Chen, You-Qin AU - Chen YQ AD - Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH, 44106, USA. FAU - Peng, Jun AU - Peng J AD - Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. pjunlab@hotmail.com. AD - Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China. pjunlab@hotmail.com. AD - Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, 350122, China. pjunlab@hotmail.com. FAU - Chen, Ke-Ji AU - Chen KJ AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. kjchenvip@163.com. LA - eng PT - Journal Article DEP - 20191227 PL - China TA - Chin J Integr Med JT - Chinese journal of integrative medicine JID - 101181180 RN - 0 (Drugs, Chinese Herbal) RN - 0 (Smad3 Protein) RN - 0 (Smad3 protein, rat) RN - 0 (Tgfb1 protein, rat) RN - 0 (Transforming Growth Factor beta1) SB - IM MH - Animals MH - Aorta, Thoracic/drug effects MH - Blood Pressure/drug effects MH - Disease Models, Animal MH - Drugs, Chinese Herbal/*pharmacology MH - Hypertension/*drug therapy MH - Male MH - Rats MH - Rats, Inbred SHR MH - Signal Transduction/*drug effects MH - Smad3 Protein/*metabolism MH - Transforming Growth Factor beta1/*metabolism MH - Vascular Remodeling/*drug effects OTO - NOTNLM OT - Chinese medicine OT - Qingxuan Jiangya Decoction OT - transforming growth factor-beta 1/Smad signaling pathway OT - vascular structural remodeling EDAT- 2019/12/29 06:00 MHDA- 2020/09/25 06:00 CRDT- 2019/12/29 06:00 PHST- 2017/11/15 00:00 [accepted] PHST- 2019/12/29 06:00 [pubmed] PHST- 2020/09/25 06:00 [medline] PHST- 2019/12/29 06:00 [entrez] AID - 10.1007/s11655-019-2705-7 [pii] AID - 10.1007/s11655-019-2705-7 [doi] PST - ppublish SO - Chin J Integr Med. 2020 Mar;26(3):180-187. doi: 10.1007/s11655-019-2705-7. Epub 2019 Dec 27.