PMID- 31883213
OWN - NLM
STAT- MEDLINE
DCOM- 20201105
LR  - 20201105
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 35
IP  - 4
DP  - 2020 Jun
TI  - Depicting the binding of furazolidone/furacilin with DNA by multiple 
      spectroscopies, voltammetric as well as molecular docking.
PG  - 493-502
LID - 10.1002/bio.3754 [doi]
AB  - The interaction between DNA and furazolidone/furacillin was investigated using 
      various analytical techniques including spectroscopy and electroanalysis and 
      molecular modelling. With the aid of acridine orange (AO), the fluorescence 
      lifetimes of DNA-AO, DNA-furazolidone/furacillin-AO remained almost the same, 
      which proved that the ground state complex was formed due to 
      furazolidone/furacillin binding with DNA. Circular dichroism spectra and Fourier 
      transform infrared spectroscopy showed that the second structure of DNA changed. 
      Viscosity experiments presented that relative viscosity of DNA was increased with 
      the increasing concentrations of furazolidone and almost unchanged for furacilin. 
      In addition, the results of melting temperature (T(m) ), ionic strength, site 
      competition experiments, cyclic voltammetry, and molecular docking all proved the 
      intercalation binding mode for furazolidone and groove binding mode for 
      furacilin. The binding constants (K(a) ) obtained from Wolfe-Shimmer equation 
      were calculated as 3.66 x 10(4) L mol(-1) and 3.95 x 10(4) L mol(-1) for 
      furazolidone-DNA and furacilin-DNA, respectively.
CI  - (c) 2019 John Wiley & Sons, Ltd.
FAU - Bi, Shuyun
AU  - Bi S
AUID- ORCID: 0000-0002-4658-0480
AD  - College of Chemistry, Changchun Normal University, Changchun, China.
FAU - Sun, Xiaoyue
AU  - Sun X
AD  - College of Chemistry, Changchun Normal University, Changchun, China.
FAU - Li, Xu
AU  - Li X
AD  - College of Chemistry, Changchun Normal University, Changchun, China.
FAU - Zhao, Rui
AU  - Zhao R
AD  - College of Chemistry, Changchun Normal University, Changchun, China.
FAU - Shao, Di
AU  - Shao D
AD  - College of Chemistry, Changchun Normal University, Changchun, China.
LA  - eng
GR  - 20170101020JC/Jilin Natural Science Foundation/
PT  - Journal Article
DEP - 20191228
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 5J9CPU3RE0 (Furazolidone)
RN  - 9007-49-2 (DNA)
RN  - X8XI70B5Z6 (Nitrofurazone)
SB  - IM
MH  - Binding Sites
MH  - Circular Dichroism
MH  - DNA/*chemistry
MH  - *Electrochemical Techniques
MH  - Furazolidone/*chemistry
MH  - *Molecular Docking Simulation
MH  - Nitrofurazone/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Viscosity
OTO - NOTNLM
OT  - DNA
OT  - binding mode
OT  - furacilin
OT  - furazolidone
EDAT- 2019/12/29 06:00
MHDA- 2020/11/06 06:00
CRDT- 2019/12/29 06:00
PHST- 2019/08/10 00:00 [received]
PHST- 2019/10/12 00:00 [revised]
PHST- 2019/11/25 00:00 [accepted]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/11/06 06:00 [medline]
PHST- 2019/12/29 06:00 [entrez]
AID - 10.1002/bio.3754 [doi]
PST - ppublish
SO  - Luminescence. 2020 Jun;35(4):493-502. doi: 10.1002/bio.3754. Epub 2019 Dec 28.