PMID- 31883471
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20201109
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 97
DP  - 2020 Feb
TI  - Functional characterization of grass carp (Ctenopharyngodon idella) interleukin-2 
      in head kidney leukocytes.
PG  - 500-508
LID - S1050-4648(19)31216-1 [pii]
LID - 10.1016/j.fsi.2019.12.077 [doi]
AB  - Interleukin (IL)-2 belongs to the four-helix bundle cytokine family and plays key 
      roles in growth, survival, activation-induced cell death and differentiation of 
      the immune cells. In cyprinid fish, only common carp interleukin-2 (il2) has been 
      cloned because of relatively low sequence homology between carp Il-2 and its 
      homologs in other fish species. In the present study, the coding sequence of 
      grass carp Il-2 (gcIl-2) was cloned and its identity was verified via 
      bioinformatic analysis. Tissue distribution study showed that grass carp il2 
      (gcil2) mRNA was expressed in thymus, head kidney and gill with relatively high 
      levels. Recombinant gcIl-2 (rgcIl-2) protein was subsequently prepared by using a 
      prokaryotic expression system followed by a refolding method. The purified 
      rgcIl-2 displayed an ability to stimulate the cell proliferation along with an 
      increased mRNA expression of cd4l but not cd8a, igm or mcsfr in grass carp head 
      kidney leukocytes (HKLs), suggesting the possible involvement of gcIl-2 in T 
      helper (Th) cell proliferation. In the same cell model, rgcIl-2 significantly 
      enhanced mRNA expression of some cytotoxic molecules including perforin-like 
      protein 2, granzyme B-like and Fas ligand, indicating the modulation of cytotoxic 
      cells by gcIl-2 in grass carp HKLs. Besides, gene expression of regulatory T 
      (Treg) cell- and Th1/2 cell-related cytokines or transcription factors was 
      detected in grass carp HKLs treated by rgcIl-2. Results showed that rgcIL2 
      treatment increased the mRNA expression of foxp3, cd25l, ifng2, il12p35, tbet, 
      tnfa, il2, il4/13a, il4/13b and gata3l in HKLs, implying the regulatory roles of 
      Il-2 in the expression of these immune genes and its possible involvement in 
      differentiation of Treg and Th1/2 cells. These observations together with the 
      related studies in other fishes suggest the existence of cytotoxic cells, Treg 
      and Th1/2 subpopulations in fish species and the functional roles of Il-2 in 
      these cells.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Lv, Mengyuan
AU  - Lv M
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China. Electronic address: wangxyhku@uestc.edu.cn.
FAU - Qiu, Xingyang
AU  - Qiu X
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Zeng, Tingting
AU  - Zeng T
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Feng, Shiyu
AU  - Feng S
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Yin, Licheng
AU  - Yin L
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Zhang, Anying
AU  - Zhang A
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
FAU - Yang, Kun
AU  - Yang K
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic Science and Technology of China, Chengdu, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20191225
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Carps/*immunology
MH  - Computational Biology
MH  - Cytokines/immunology
MH  - Fish Proteins/genetics/immunology
MH  - Gene Expression
MH  - Head Kidney/*cytology/immunology
MH  - Interleukin-2/genetics/*immunology
MH  - Leukocytes/*immunology
MH  - RNA, Messenger/immunology
MH  - Signal Transduction
OTO - NOTNLM
OT  - Cytotoxic cell
OT  - Gene expression
OT  - Grass carp
OT  - Head kidney leucocytes
OT  - Il-2
OT  - Proliferation
OT  - T-helper cell
EDAT- 2019/12/29 06:00
MHDA- 2020/11/11 06:00
CRDT- 2019/12/29 06:00
PHST- 2019/07/17 00:00 [received]
PHST- 2019/12/17 00:00 [revised]
PHST- 2019/12/24 00:00 [accepted]
PHST- 2019/12/29 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/12/29 06:00 [entrez]
AID - S1050-4648(19)31216-1 [pii]
AID - 10.1016/j.fsi.2019.12.077 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 Feb;97:500-508. doi: 10.1016/j.fsi.2019.12.077. Epub 
      2019 Dec 25.