PMID- 31883610 OWN - NLM STAT- MEDLINE DCOM- 20210421 LR - 20210421 IS - 2224-6614 (Electronic) VI - 28 IP - 1 DP - 2020 Jan TI - Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins. PG - 74-83 LID - S1021-9498(19)30049-3 [pii] LID - 10.1016/j.jfda.2019.05.001 [doi] AB - Mogrosides are the major triterpenoidal saponins found in swingle, the fruit of Siraitia grosvenorii, which have recently been widely used throughout the world as natural food sweeteners. Among this class of compounds, mogroside III E (MG III E) exhibits the most intense sweetness, and it was also found to effectively regulate blood glucose levels. However, the relative abundance of naturally occurring MG III E is low compared to other mogrosides. Therefore, the purpose of this study was to enrich MG III E through biotransformation of fruit extracts and to develop a reliable method for its purification. We used HPLC coupled with mass spectrometry and nuclear magnetic resonance spectroscopy for metabolite analysis and identified MG III E as a major metabolite of Ganoderma lucidum mycelium. This organism converts the most abundant mogroside, mogroside V, to MG III E via a deglycosylation reaction; high levels of beta-glucosidase activities were also detected. In addition, we established an efficient purification method for MG III E using HP-20 macroporous resin. Optimization of the method was accomplished by kinetic model fitting, dynamic adsorption studies, and desorption experiments. The purity of MG III E was increased from 11.71% to 54.19%, with a 70%-76% recovery rate, and the scaled-up purification process allowed us to harvest 17.38 g of MG III E with 55.14% purity and a 74.71% of recovery rate. Therefore, our low cost, time-saving, easy to scale-up procedure for isolating MG III E could be applicable in industrial processes. CI - Copyright (c) 2019. Published by Elsevier Taiwan LLC. FAU - Chiu, Chun-Hui AU - Chiu CH AD - Institute of Health Industry and Technology, Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan; Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan. FAU - Wang, Reuben AU - Wang R AD - Department of Food Science, Tunghai University, No. 1727, Sec. 4 Taiwan Boulevard, Xitun District, Taichung, Taiwan. FAU - Zhuang, Shasha AU - Zhuang S AD - Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan. FAU - Lin, Pei-Yin AU - Lin PY AD - Joint Center for Instruments and Researches, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan. FAU - Lo, Yi-Chen AU - Lo YC AD - Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan. Electronic address: loyichen@ntu.edu.tw. FAU - Lu, Ting-Jang AU - Lu TJ AD - Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan. Electronic address: tjlu@ntu.edu.tw. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190529 PL - China (Republic : 1949- ) TA - J Food Drug Anal JT - Journal of food and drug analysis JID - 101630927 RN - 0 (Glucosides) RN - 0 (Saponins) RN - 0 (Sweetening Agents) RN - 0 (Triterpenes) RN - 0 (mogroside IIIE) SB - IM MH - Biotransformation MH - Cucurbitaceae/*chemistry MH - Fruit/chemistry MH - Glucosides/*isolation & purification/metabolism MH - Mycelium MH - Reishi/*metabolism MH - Saponins/*metabolism MH - Sweetening Agents MH - Triterpenes/*isolation & purification/metabolism OTO - NOTNLM OT - Biotransformation OT - Diaion(R) HP-20 OT - Ganoderma lucidum OT - Mogroside III E OT - Sweetener EDAT- 2019/12/31 06:00 MHDA- 2021/04/22 06:00 CRDT- 2019/12/30 06:00 PHST- 2018/12/02 00:00 [received] PHST- 2019/05/08 00:00 [revised] PHST- 2019/05/10 00:00 [accepted] PHST- 2019/12/30 06:00 [entrez] PHST- 2019/12/31 06:00 [pubmed] PHST- 2021/04/22 06:00 [medline] AID - S1021-9498(19)30049-3 [pii] AID - 10.1016/j.jfda.2019.05.001 [doi] PST - ppublish SO - J Food Drug Anal. 2020 Jan;28(1):74-83. doi: 10.1016/j.jfda.2019.05.001. Epub 2019 May 29.