PMID- 31884243
OWN - NLM
STAT- MEDLINE
DCOM- 20210224
LR  - 20210224
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 77
DP  - 2020 Mar
TI  - Fish oil up-regulates hepatic autophagy in rats with chronic ethanol consumption.
PG  - 108314
LID - S0955-2863(19)30962-3 [pii]
LID - 10.1016/j.jnutbio.2019.108314 [doi]
AB  - In this study, we examined the regulation of autophagy by fish oil in rats under 
      ethanol-containing diets. Thirty male Wistar rats (8-week-old) were divided into 
      six groups and fed a control diet or an ethanol-containing diet, which was 
      adjusted with fish oil to replace 25% or 57% of the olive oil. After 8 weeks, 
      rats in the E (ethanol diet) group showed the significantly higher plasma 
      aspartate transaminase (AST) and alanine transaminase (ALT) activities, protein 
      expression of cytochrome P450 2E1 (CYP2E1), and levels of hepatic inflammatory 
      cytokines. However, all of those items had significantly decreased in the EF25 
      (ethanol with 25% fish oil) and EF57 (ethanol with 57% fish oil) groups. As to 
      autophagic indicators, protein expressions of mammalian target of rapamycin 
      (mTOR), Unc-51-like autophagy activating kinase 1 (ULK1) and p62 were 
      significantly increased in the E group. Conversely, the protein expressions of 
      light chain 3II (LC3II)/LC3I and Beclin1 were significantly decreased in the E 
      group. On the other hand, protein expressions of phosphorylated Akt, mTOR, ULK1, 
      and p62 were down-regulated, protein expressions of LC3II/LC3I and Beclin1 were 
      conversely up-regulated in the EF25 and EF57 groups. Fish oil activated hepatic 
      autophagy via inhibiting the Akt signaling pathway, which exerted protective 
      effects against ethanol-induced liver injury in rats.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Lu, Nien-Shan
AU  - Lu NS
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Chiu, Wan-Chun
AU  - Chiu WC
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Chen, Ya-Ling
AU  - Chen YL
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Peng, Hsiang-Chi
AU  - Peng HC
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, 
      Taiwan.
FAU - Shirakawa, Hitoshi
AU  - Shirakawa H
AD  - Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku 
      University, Sendai, 980-8572, Japan.
FAU - Yang, Suh-Ching
AU  - Yang SC
AD  - School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, 
      Taiwan; Research Center of Geriatric Nutrition, College of Nutrition, Taipei 
      Medical University, Taipei 11031, Taiwan; Nutrition Research Center, Taipei 
      Medical University Hospital, Taipei 11031, Taiwan. Electronic address: 
      sokei@tmu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191216
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Antioxidants)
RN  - 0 (Beclin-1)
RN  - 0 (Becn1 protein, rat)
RN  - 0 (Fish Oils)
RN  - 0 (Olive Oil)
RN  - 0 (Triglycerides)
RN  - 3K9958V90M (Ethanol)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - EC 2.7.11.1 (Autophagy-Related Protein-1 Homolog)
RN  - EC 2.7.11.1 (ULK1 protein, rat)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Alanine Transaminase/metabolism
MH  - Alcohol Drinking/*adverse effects
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Aspartate Aminotransferases/metabolism
MH  - *Autophagy
MH  - Autophagy-Related Protein-1 Homolog/metabolism
MH  - Beclin-1/metabolism
MH  - Cholesterol/metabolism
MH  - Cytochrome P-450 CYP2E1/metabolism
MH  - Dietary Supplements
MH  - Disease Models, Animal
MH  - Ethanol
MH  - Fish Oils/*pharmacology
MH  - Glutathione/metabolism
MH  - Inflammation
MH  - Lipid Metabolism
MH  - Liver/*metabolism
MH  - Liver Diseases, Alcoholic/metabolism
MH  - Male
MH  - Olive Oil/pharmacology
MH  - *Oxidative Stress
MH  - Rats
MH  - Rats, Wistar
MH  - Triglycerides/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Akt singalling pathway
OT  - Alcoholic liver disease
OT  - Autophagy
OT  - Fish oil
OT  - Wistar rats
EDAT- 2019/12/31 06:00
MHDA- 2021/02/25 06:00
CRDT- 2019/12/30 06:00
PHST- 2019/09/30 00:00 [received]
PHST- 2019/11/12 00:00 [revised]
PHST- 2019/11/27 00:00 [accepted]
PHST- 2019/12/31 06:00 [pubmed]
PHST- 2021/02/25 06:00 [medline]
PHST- 2019/12/30 06:00 [entrez]
AID - S0955-2863(19)30962-3 [pii]
AID - 10.1016/j.jnutbio.2019.108314 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2020 Mar;77:108314. doi: 10.1016/j.jnutbio.2019.108314. Epub 2019 
      Dec 16.