PMID- 31884362
OWN - NLM
STAT- MEDLINE
DCOM- 20201224
LR  - 20201224
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 386
DP  - 2020 Mar 15
TI  - Microcystin-LR triggers different endoplasmic reticulum stress pathways in the 
      liver, ovary, and offspring of zebrafish (Danio rerio).
PG  - 121939
LID - S0304-3894(19)31893-X [pii]
LID - 10.1016/j.jhazmat.2019.121939 [doi]
AB  - The existence of microcystins (MCs), the secondary metabolite of cyanobacteria, 
      has become a growing public health concern. Previous researches have proved that 
      MCs can trigger endoplasmic reticulum stress (ERS), but the underlying mechanisms 
      remain unclear. In the present study, adult female zebrafish were exposed to 
      MC-LR (0, 1, 5 and 20 mug/L) for 30 d, and the offspring derived from the treated 
      females and healthy males were cultured in water without MC-LR until 96 h post 
      fertilization (hpf). Our data suggested that MC-LR causes a significant increase 
      in the eif2s1a, atf4, and eif2ak3 transcription levels in the liver and ovary. 
      The mRNA levels of atf4, atf6, bcl-2, hspa5, eif2s1a and eif2ak3 upregulated 
      notably in the offspring. JNK phosphorylation level and cleaved-caspase3 protein 
      expression elevated obviously in the liver and ovary, but had no remarkable 
      change in the offspring. Furthermore, TUNEL results showed that MC-LR 
      significantly induced apoptosis in the liver and ovary, while acridine orange 
      (AO) staining indicated that MC-LR did not cause abnormal apoptosis in offspring. 
      Concisely, the present study indicated that MC-LR leads to apoptosis through 
      different ERS pathways in the liver, ovary and offspring, and also provides a new 
      perspective for understanding the apoptosis caused by MC-LR.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zhan, Chunhua
AU  - Zhan C
AD  - College of Fisheries, Huazhong Agricultural University, Hubei Provincial 
      Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China.
FAU - Liu, Wanjing
AU  - Liu W
AD  - College of Fisheries, Huazhong Agricultural University, Hubei Provincial 
      Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China.
FAU - Zhang, Feng
AU  - Zhang F
AD  - College of Fisheries, Huazhong Agricultural University, Hubei Provincial 
      Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China.
FAU - Zhang, Xuezhen
AU  - Zhang X
AD  - College of Fisheries, Huazhong Agricultural University, Hubei Provincial 
      Engineering Laboratory for Pond Aquaculture, Wuhan 430070, China. Electronic 
      address: xuezhen@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191219
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (Water Pollutants, Chemical)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Embryo, Nonmammalian/*drug effects/metabolism/pathology
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Female
MH  - Liver/*drug effects/metabolism/pathology
MH  - Marine Toxins/*toxicity
MH  - Microcystins/*toxicity
MH  - Ovary/*drug effects/metabolism/pathology
MH  - Water Pollutants, Chemical/*toxicity
MH  - Zebrafish/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Microcystins
OT  - Organ-specific
OT  - Parental transmission
OT  - Zebrafish
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2019/12/31 06:00
MHDA- 2020/12/29 06:00
CRDT- 2019/12/30 06:00
PHST- 2019/09/18 00:00 [received]
PHST- 2019/11/12 00:00 [revised]
PHST- 2019/12/17 00:00 [accepted]
PHST- 2019/12/31 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2019/12/30 06:00 [entrez]
AID - S0304-3894(19)31893-X [pii]
AID - 10.1016/j.jhazmat.2019.121939 [doi]
PST - ppublish
SO  - J Hazard Mater. 2020 Mar 15;386:121939. doi: 10.1016/j.jhazmat.2019.121939. Epub 
      2019 Dec 19.