PMID- 31885501
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2019
DP  - 2019
TI  - Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies 
      Complicated by Acute Chorioamnionitis.
PG  - 8687120
LID - 10.1155/2019/8687120 [doi]
LID - 8687120
AB  - Chorioamnionitis (CAM) is primarily a polymicrobial bacterial infection involving 
      chorionic and amniotic membranes that is associated with increased risk of 
      preterm delivery. Epoxyeicosatrienoic acids (EETs) are eicosanoids generated from 
      arachidonic acid by cytochrome P450 enzymes and further metabolized mainly by 
      soluble epoxide hydrolase (sEH) to produce dihydroxyeicosatrienoic acids (DHETs). 
      As a consequence of this metabolism of EETs, sEH reportedly exacerbates several 
      disease states; however, its role in CAM remains unclear. The objectives of this 
      study were to (1) determine the localization of sEH and compare the changes it 
      undergoes in the gestational tissues (placentas and fetal membranes) of women 
      with normal-term pregnancies and those with pregnancies complicated by acute CAM; 
      (2) study the effects of lipopolysaccharide (LPS) on the expression of sEH in the 
      human gestational tissues; and (3) investigate the effect of 
      12-(3-adamantan-1-yl-ureido)-dodecanoic acid (AUDA), a specific sEH inhibitor, on 
      LPS-induced changes in 14,15-DHET and cytokines such as interleukin- (IL-) 1beta and 
      IL-6 in human gestational tissues in vitro and in pregnant mice. We found that 
      women with pregnancies complicated by acute CAM had higher levels of sEH mRNA and 
      protein in fetal membranes and villous tissues compared to those in women with 
      normal-term pregnancies without CAM. Furthermore, fetal membrane and villous 
      explants treated with LPS had higher tissue levels of sEH mRNA and protein and 
      14,15-DHET than those present in the vehicle controls, while the administration 
      of AUDA in the media attenuated the LPS-induced production of 14,15-DHET in 
      tissue homogenates and IL-1beta and IL-6 in the media of explant cultures. 
      Administration of AUDA also reduced the LPS-induced changes of 14,15-DHET, IL-1beta, 
      and IL-6 in the placentas of pregnant mice. Together, these results suggest that 
      sEH participates in the inflammatory changes in human gestational tissues in 
      pregnancies complicated by acute CAM.
CI  - Copyright (c) 2019 Tai-Ho Hung et al.
FAU - Hung, Tai-Ho
AU  - Hung TH
AUID- ORCID: 0000-0003-2354-7060
AD  - Department of Obstetrics and Gynecology, Taipei Chang Gung Memorial Hospital, 
      Taiwan.
AD  - Department of Obstetrics and Gynecology, Keelung Chang Gung Memorial Hospital, 
      Taiwan.
AD  - College of Medicine, Chang Gung University, Taoyuan, Taiwan.
FAU - Chen, Szu-Fu
AU  - Chen SF
AUID- ORCID: 0000-0002-8398-883X
AD  - Department of Physical Medicine and Rehabilitation, Cheng Hsin General Hospital, 
      Taipei, Taiwan.
FAU - Wu, Chun-Hu
AU  - Wu CH
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
FAU - Kao, Chuan-Chi
AU  - Kao CC
AD  - Department of Obstetrics and Gynecology, Keelung Chang Gung Memorial Hospital, 
      Taiwan.
FAU - Wu, Chung-Pu
AU  - Wu CP
AUID- ORCID: 0000-0002-2434-3361
AD  - Graduate Institute of Biomedical Sciences, Department of Physiology and 
      Pharmacology and Molecular Medicine Research Center, College of Medicine, Chang 
      Gung University, Taoyuan, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20191205
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (14,15-dihydroxyeicosatrienoic acid)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - FC398RK06S (8,11,14-Eicosatrienoic Acid)
SB  - IM
MH  - 8,11,14-Eicosatrienoic Acid/analogs & derivatives/metabolism
MH  - Amnion/drug effects/metabolism
MH  - Chorioamnionitis/*enzymology/metabolism
MH  - Epoxide Hydrolases/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Placenta/drug effects/metabolism
MH  - Pregnancy
PMC - PMC6915158
COIS- All the authors declare that they have no conflicts of interest.
EDAT- 2019/12/31 06:00
MHDA- 2020/06/09 06:00
PMCR- 2019/12/05
CRDT- 2019/12/31 06:00
PHST- 2019/07/03 00:00 [received]
PHST- 2019/10/17 00:00 [revised]
PHST- 2019/10/26 00:00 [accepted]
PHST- 2019/12/31 06:00 [entrez]
PHST- 2019/12/31 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/12/05 00:00 [pmc-release]
AID - 10.1155/2019/8687120 [doi]
PST - epublish
SO  - Mediators Inflamm. 2019 Dec 5;2019:8687120. doi: 10.1155/2019/8687120. 
      eCollection 2019.