PMID- 31885577
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-8450 (Print)
IS  - 1687-8469 (Electronic)
IS  - 1687-8450 (Linking)
VI  - 2019
DP  - 2019
TI  - Expression Analysis of the Mediators of Epithelial to Mesenchymal Transition and 
      Early Risk Assessment of Therapeutic Failure in Laryngeal Carcinoma.
PG  - 5649846
LID - 10.1155/2019/5649846 [doi]
LID - 5649846
AB  - Laryngeal squamous cell carcinoma (LSCC) is an aggressive malignancy which lacks 
      early predictors of prognosis. Here, we hypothesized that expression and 
      prognostic characterization of the critical mediators of epithelial to 
      mesenchymal transition (EMT) may provide key information in this regard. Linear 
      regression and multiple correspondence analyses were performed on 
      immunohistochemical data obtained from 20 invasive tumors. Principal component 
      and unsupervised hierarchical clustering were used to analyze the dataset 
      patterns associating with LSCC metastatic profile. Survival and death risk 
      assessments were performed using Kaplan-Meier and hazard ratio tests. Data mining 
      analysis using CHAID decision tree and logistic regression analysis was applied 
      to define the predictive value of the risk factors of tumor aggressiveness. Our 
      analyses showed, that in invasive LSCC tumors, cells associating with a 
      mesenchymal profile were likely to exhibit enhanced NOS2, TGF-beta, and IL-17A 
      expression levels, concomitantly to NF-kappaB nuclear translocation. IHC data 
      deciphering determined that EMT induction was also linked to the enrichment of 
      the tumors with CD68+ populations and IL-10 signal. Strikingly, dataset cluster 
      analysis showed that these signatures could define distinct patterns of invasive 
      tumors, where NOS2 associated with IL-10 expression, and TGF-beta and IL-17A signals 
      associated with MMP-9 activation. Decision tree analysis identified IL-17A as a 
      possible predictor of LSCC aggressiveness. Altogether, our results show that 
      distinct immunological patterns would support the acquisition of EMT features in 
      invasive LSCC and suggest that IL-17A may be useful in the early identification 
      of patients "at-risk" of therapeutic failure.
CI  - Copyright (c) 2019 Nora Kariche et al.
FAU - Kariche, Nora
AU  - Kariche N
AUID- ORCID: 0000-0002-8877-2691
AD  - Department of Cell and Molecular Biology, Team Cytokines and Nitric Oxide 
      Synthases. Faculty of Biology, University Houari Boumediene USTHB, Algiers, 
      Algeria.
FAU - Moulai, Nabila
AU  - Moulai N
AD  - Central Laboratory for Anatomopathology, Frantz Fanon Hospital, Blida, Algeria.
FAU - Sellam, Leila-Sarah
AU  - Sellam LS
AD  - Department of Cell and Molecular Biology, Team Cytokines and Nitric Oxide 
      Synthases. Faculty of Biology, University Houari Boumediene USTHB, Algiers, 
      Algeria.
FAU - Benyahia, Samir
AU  - Benyahia S
AD  - Oto-Rhyno-laryngology Department, Mustapha Pacha Hospital, Algiers, Algeria.
FAU - Ouahioune, Wahiba
AU  - Ouahioune W
AD  - Central Laboratory for Anatomopathology, Frantz Fanon Hospital, Blida, Algeria.
FAU - Djennaoui, Djamel
AU  - Djennaoui D
AD  - Oto-Rhyno-laryngology Department, Mustapha Pacha Hospital, Algiers, Algeria.
FAU - Touil-Boukoffa, Chafia
AU  - Touil-Boukoffa C
AD  - Department of Cell and Molecular Biology, Team Cytokines and Nitric Oxide 
      Synthases. Faculty of Biology, University Houari Boumediene USTHB, Algiers, 
      Algeria.
FAU - Bourouba, Mehdi
AU  - Bourouba M
AUID- ORCID: 0000-0002-2304-5899
AD  - Department of Cell and Molecular Biology, Team Cytokines and Nitric Oxide 
      Synthases. Faculty of Biology, University Houari Boumediene USTHB, Algiers, 
      Algeria.
LA  - eng
PT  - Journal Article
DEP - 20191206
PL  - Egypt
TA  - J Oncol
JT  - Journal of oncology
JID - 101496537
PMC - PMC6926423
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2019/12/31 06:00
MHDA- 2019/12/31 06:01
PMCR- 2019/12/06
CRDT- 2019/12/31 06:00
PHST- 2019/05/18 00:00 [received]
PHST- 2019/07/26 00:00 [revised]
PHST- 2019/08/10 00:00 [accepted]
PHST- 2019/12/31 06:00 [entrez]
PHST- 2019/12/31 06:00 [pubmed]
PHST- 2019/12/31 06:01 [medline]
PHST- 2019/12/06 00:00 [pmc-release]
AID - 10.1155/2019/5649846 [doi]
PST - epublish
SO  - J Oncol. 2019 Dec 6;2019:5649846. doi: 10.1155/2019/5649846. eCollection 2019.