PMID- 31887117
OWN - NLM
STAT- MEDLINE
DCOM- 20200409
LR  - 20200409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 12
DP  - 2019
TI  - Glucocorticoid and dietary effects on mucosal microbiota in canine inflammatory 
      bowel disease.
PG  - e0226780
LID - 10.1371/journal.pone.0226780 [doi]
LID - e0226780
AB  - The pathogenesis of canine inflammatory bowel disease (IBD) involves complex 
      interactions between mucosal immunity and the intestinal microbiota. 
      Glucocorticoids are commonly administered to reduce mucosal inflammation and 
      gastrointestinal signs. The study objective was to evaluate the effects of diet 
      and oral prednisone on the spatial distribution of mucosal bacteria in IBD dogs. 
      Eight dogs diagnosed with IBD were treated with immunosuppressive doses of 
      prednisone. The mucosal microbiota from endoscopic biopsies of IBD dogs and 
      healthy controls (HC; n = 15 dogs) was evaluated by fluorescence in situ 
      hybridization (FISH) targeting the 16S rRNA genes of total bacteria and bacterial 
      species relevant in canine/human IBD. Apicaljunction protein (AJP) expression 
      using immunohistochemistry investigated the effect of medical therapy on 
      intestinal barrier integrity. All IBD dogs had a reduction in GI signs following 
      diet and prednisone therapy compared with baseline CIBDAI scores (P < 0.05). The 
      mucosal microbiota of HC and diseased dogs was most abundant in free and adherent 
      mucus. Only Lactobacilli were increased (P < 0.05) in the adherent mucus of IBD 
      dogs compared to HC. The spatial distribution of mucosal bacteria was 
      significantly different (P < 0.05) in IBD dogs following prednisone therapy, with 
      higher numbers of Bifidobacteria and Streptococci detected across all mucosal 
      compartments and increased numbers of Bifidobacterium spp., Faecalibacterium 
      spp., and Streptococcus spp. present within adherent mucus. Differences in 
      intestinal AJPs were detected with expression of occludin increased (P < 0.05) in 
      IBD dogs versus HC. The expressions of occludin and E-cadherin were increased but 
      zonulin decreased (P < 0.05 for each) in IBD dogs following prednisone therapy. 
      In conclusion, the spatial distribution of mucosal bacteria differs between IBD 
      and HC dogs, and in response to diet and glucocorticoid administration. Medical 
      therapy was associated with beneficial changes in microbial community structure 
      and enhanced mucosal epithelial AJP expression.
FAU - Atherly, Todd
AU  - Atherly T
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - Rossi, Giacomo
AU  - Rossi G
AD  - School of Biosciences and Veterinary Medicine, University of Camerino, Macerata, 
      Italy.
FAU - White, Robin
AU  - White R
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - Seo, Yeon-Jung
AU  - Seo YJ
AD  - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa, United States of America.
FAU - Wang, Chong
AU  - Wang C
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
FAU - Ackermann, Mark
AU  - Ackermann M
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa, United States of America.
FAU - Breuer, Mary
AU  - Breuer M
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, Iowa, United States of America.
FAU - Allenspach, Karin
AU  - Allenspach K
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
FAU - Mochel, Jonathan P
AU  - Mochel JP
AUID- ORCID: 0000-0002-0997-3111
AD  - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa, United States of America.
FAU - Jergens, Albert E
AU  - Jergens AE
AUID- ORCID: 0000-0003-2375-8685
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa 
      State University, Ames, Iowa, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20191230
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cadherins)
RN  - 0 (Glucocorticoids)
RN  - 0 (Occludin)
RN  - VB0R961HZT (Prednisone)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/isolation & purification
MH  - Cadherins/metabolism
MH  - Demography
MH  - *Diet
MH  - *Dog Diseases/diet therapy/drug therapy/microbiology
MH  - Dogs
MH  - Glucocorticoids/*therapeutic use
MH  - Inflammatory Bowel Diseases/diet therapy/drug therapy/microbiology/*veterinary
MH  - Intestinal Mucosa/immunology/*microbiology
MH  - *Microbiota
MH  - Occludin/metabolism
MH  - Prednisone/therapeutic use
PMC - PMC6936794
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/12/31 06:00
MHDA- 2020/04/10 06:00
PMCR- 2019/12/30
CRDT- 2019/12/31 06:00
PHST- 2019/08/21 00:00 [received]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2019/12/31 06:00 [entrez]
PHST- 2019/12/31 06:00 [pubmed]
PHST- 2020/04/10 06:00 [medline]
PHST- 2019/12/30 00:00 [pmc-release]
AID - PONE-D-19-23630 [pii]
AID - 10.1371/journal.pone.0226780 [doi]
PST - epublish
SO  - PLoS One. 2019 Dec 30;14(12):e0226780. doi: 10.1371/journal.pone.0226780. 
      eCollection 2019.