PMID- 31891789 OWN - NLM STAT- MEDLINE DCOM- 20210104 LR - 20210104 IS - 1096-1186 (Electronic) IS - 1043-6618 (Linking) VI - 152 DP - 2020 Feb TI - Resveratrol derivative excited postsynaptic potentiation specifically via PKCbeta-NMDA receptor mediation. PG - 104618 LID - S1043-6618(19)31347-7 [pii] LID - 10.1016/j.phrs.2019.104618 [doi] AB - Several decades have passed since resveratrol (RSV) was first identified in red wine. Researchers have reported the pleiotropic anti-oxidant, anti-inflammatory, anti-cancer, anti-aging, and neuronal protective effects of resveratrol and its glycosylated derivative. However, few studies have distinguished the minute differences in the properties between resveratrol and its glycosylated derivative in terms of synaptic plasticity. As an abundant natural product of glycosylated resveratrol, the derivative 2,3,4',5-tetrahydroxystilbene-2-O-beta-d-glucoside (TSG) has been determined to be a better option for long-term potentiation (LTP) in the hippocampus under physiological and pathological conditions than resveratrol. TSG, as well as its parent molecule RSV, could elicit early-LTP and recover fast excitatory postsynaptic potentials (EPSPs) in the hippocampus. Using various modalities, including pre- and post-whole-cell patch clamping techniques in the calyx of Held, pharmacological inhibition of the N-methyl-d-aspartic acid receptor (NMDAr) and the alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAr) as well as protein kinase C (PKC) activation, we demonstrated that TSG, unlike RSV, could merely promote NMDA-mediated EPSC via PKCbeta cascade. Our results provide new knowledge that glycosylation of resveratrol could significantly improve its specificity in promoting sole NMDAr mediation of EPSPs, in addition to improving solubility and resistance against oxidation in vivo. These observations could contribute to further exploration of pharmaceutical evaluation of glycosylated stilbene in the future. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - Tong, Jia AU - Tong J AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Gao, Jingjing AU - Gao J AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Liu, Qingzhen AU - Liu Q AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - He, Chenyang AU - He C AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Zhao, Xing AU - Zhao X AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Qi, Yawei AU - Qi Y AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Yuan, Tiangang AU - Yuan T AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. FAU - Li, Pengyan AU - Li P AD - China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, 100101, China. FAU - Niu, Ming AU - Niu M AD - China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, 100101, China. FAU - Wang, Dongyao AU - Wang D AD - China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, 100101, China. FAU - Zhang, Le AU - Zhang L AD - China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, 100101, China. FAU - Li, Weiyong AU - Li W AD - Department of Pharmacy, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430000, China. FAU - Wang, Jiabo AU - Wang J AD - China Military Institute of Chinese Medicine, 302 Military Hospital, Beijing, 100101, China. Electronic address: pharm_sci@126.com. FAU - Zhang, Zhongjian AU - Zhang Z AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. Electronic address: gzgz87@gmail.com. FAU - Peng, Shiyong AU - Peng S AD - Institute of Psychiatry and Neuroscience, Xinxiang Medical University, Xinxiang, Henan, 435000, China. Electronic address: sampeng@XXMU.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191228 PL - Netherlands TA - Pharmacol Res JT - Pharmacological research JID - 8907422 RN - 0 (Glucosides) RN - 0 (Receptors, N-Methyl-D-Aspartate) RN - 0 (Stilbenes) RN - 133186-49-9 (2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside) RN - EC 2.7.11.13 (Protein Kinase C beta) SB - IM MH - Animals MH - Excitatory Postsynaptic Potentials/drug effects MH - Glucosides/*pharmacology MH - Hippocampus/*drug effects/physiology MH - Long-Term Potentiation/*drug effects MH - Mice, Inbred C57BL MH - Mice, Transgenic MH - Protein Kinase C beta/physiology MH - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/physiology MH - Stilbenes/*pharmacology OTO - NOTNLM OT - 2,3,4',5-tetrahydroxystilbene-2-O-beta-d-glucoside OT - 2,3,4',5-tetrahydroxystilbene-2-O-beta-d-glucoside (PubChem CID: 73981748) OT - 3,4-Diaminopyridine (PubChem CID: 5918) OT - Bicuculline (PubChem CID: 10237) OT - Bisindolymaleimide I (PubChem CID: 2396) OT - CGP 53353 (PubChem ID: 6711154) OT - CNQX (PubChem CID: 3721046) OT - Calyx of held OT - Electrophysiology OT - L-AP5 (PubChem CID: 1617110) OT - Long-term potentiation OT - Resveratrol OT - Resveratrol (PubChem CID: 445154) OT - Strychnine (PubChem CID: 441071) OT - Synaptic plasticity OT - TEA-Cl (PubChem CID: 5946) OT - Tetrodotoxin (PubChem CID: 11174599) COIS- Declaration of Competing Interest The authors declare no biomedical financial interests or potential conflicts of interest. EDAT- 2020/01/01 06:00 MHDA- 2021/01/05 06:00 CRDT- 2020/01/01 06:00 PHST- 2019/07/11 00:00 [received] PHST- 2019/11/21 00:00 [revised] PHST- 2019/12/23 00:00 [accepted] PHST- 2020/01/01 06:00 [pubmed] PHST- 2021/01/05 06:00 [medline] PHST- 2020/01/01 06:00 [entrez] AID - S1043-6618(19)31347-7 [pii] AID - 10.1016/j.phrs.2019.104618 [doi] PST - ppublish SO - Pharmacol Res. 2020 Feb;152:104618. doi: 10.1016/j.phrs.2019.104618. Epub 2019 Dec 28.