PMID- 31893497
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20201109
IS  - 1520-5126 (Electronic)
IS  - 0002-7863 (Linking)
VI  - 142
IP  - 7
DP  - 2020 Feb 19
TI  - Double Rolling Circle Amplification Generates Physically Cross-Linked DNA Network 
      for Stem Cell Fishing.
PG  - 3422-3429
LID - 10.1021/jacs.9b11001 [doi]
AB  - Stem cells have been widely studied in cell biology and utilized in cell-based 
      therapies, and fishing stem cells from marrow is highly challenging due to the 
      ultralow content. Herein, a physically cross-linked DNA network-based cell 
      fishing strategy is reported, achieving efficient capture, 3D envelop, and 
      enzyme-triggered release of bone marrow mesenchymal stem cells (BMSCs). DNA 
      network is constructed via a double rolling circle amplification method and 
      through the intertwining and self-assembly of two strands of ultralong DNA 
      chains. DNA-chain-1 containing aptamer sequences ensures specific anchor with 
      BMSCs from marrow. Hybridization between DNA-chain-1 and DNA-chain-2 enables the 
      cross-link of cell-anchored DNA chains to form a 3D network, thus realizing cell 
      envelop and separation. DNA network creates a favorable microenvironment for 3D 
      cell culture, and remarkably the physically cross-linked DNA network shows no 
      damage to cells. DNA network is digested by nuclease, realizing the 
      deconstruction from DNA network to fragments, and achieving enzyme-triggered cell 
      release; after release, the activity of cells is well maintained. The strategy 
      provides a powerful and effective method for fishing stem cells from tens of 
      thousands of nontarget cells.
FAU - Yao, Chi
AU  - Yao C
AD  - Frontier Science Center for Synthetic Biology, Key Laboratory of Systems 
      Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin 
      University , Tianjin 300350 , People's Republic of China.
FAU - Tang, Han
AU  - Tang H
AD  - Frontier Science Center for Synthetic Biology, Key Laboratory of Systems 
      Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin 
      University , Tianjin 300350 , People's Republic of China.
FAU - Wu, Weijian
AU  - Wu W
AD  - Frontier Science Center for Synthetic Biology, Key Laboratory of Systems 
      Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin 
      University , Tianjin 300350 , People's Republic of China.
FAU - Tang, Jianpu
AU  - Tang J
AD  - Frontier Science Center for Synthetic Biology, Key Laboratory of Systems 
      Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin 
      University , Tianjin 300350 , People's Republic of China.
FAU - Guo, Weiwei
AU  - Guo W
AUID- ORCID: 0000-0002-1472-6633
AD  - College of Chemistry, Research Center for Analytical Sciences, Tianjin Key 
      Laboratory of Molecular Recognition and Biosensing, and State Key Laboratory of 
      Medicinal Chemical Biology , Nankai University , Tianjin 300071 , People's 
      Republic of China.
FAU - Luo, Dan
AU  - Luo D
AUID- ORCID: 0000-0003-2628-8391
AD  - Department of Biological & Environmental Engineering , Cornell University , 
      Ithaca , New York 14853 , United States.
FAU - Yang, Dayong
AU  - Yang D
AUID- ORCID: 0000-0002-2634-9281
AD  - Frontier Science Center for Synthetic Biology, Key Laboratory of Systems 
      Bioengineering (MOE), School of Chemical Engineering and Technology , Tianjin 
      University , Tianjin 300350 , People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200109
PL  - United States
TA  - J Am Chem Soc
JT  - Journal of the American Chemical Society
JID - 7503056
RN  - 0 (Aptamers, Nucleotide)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Aptamers, Nucleotide/chemistry
MH  - Cell Separation/*methods
MH  - DNA/*chemistry
MH  - Mesenchymal Stem Cells/chemistry/*cytology
MH  - Mice
MH  - Nucleic Acid Amplification Techniques
MH  - Nucleic Acid Hybridization
EDAT- 2020/01/02 06:00
MHDA- 2020/11/11 06:00
CRDT- 2020/01/02 06:00
PHST- 2020/01/02 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2020/01/02 06:00 [entrez]
AID - 10.1021/jacs.9b11001 [doi]
PST - ppublish
SO  - J Am Chem Soc. 2020 Feb 19;142(7):3422-3429. doi: 10.1021/jacs.9b11001. Epub 2020 
      Jan 9.