PMID- 31894287
OWN - NLM
STAT- MEDLINE
DCOM- 20200821
LR  - 20211022
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 43
IP  - 2
DP  - 2020 Feb
TI  - lncRNA XIST promotes the progression of laryngeal squamous cell carcinoma by 
      sponging miR‑144 to regulate IRS1 expression.
PG  - 525-535
LID - 10.3892/or.2019.7438 [doi]
AB  - The initiation and development of several types of cancer have been linked to 
      long non‑coding RNA (lncRNA) X inactive‑specific transcript (XIST). Yet, the 
      pattern of expression, function, as well as the molecular mechanism underlying 
      XIST in laryngeal squamous cell carcinoma (LSCC) lack characterization. 
      Therefore, the present study aimed to determine the function and putative 
      mechanism of XIST in the development of LSCC. It was revealed that the level of 
      XIST was significantly higher in LSCC tissues that were associated with advanced 
      Tumor‑Node‑Metastasis (TNM) stage and the presence of lymph node metastasis. 
      Furthermore, the ability of human LSCC TU212 cells to proliferate, form colonies, 
      migrate and invade was significantly suppressed, while cell apoptosis was 
      significantly increased following knockdown of XIST. Further investigation 
      revealed that XIST knockdown increased the expression of microRNA‑144 (miR‑144) 
      by acting as an endogenous sponge of miR‑144. Inhibition of miR‑144 caused a 
      partial reversal of the inhibitory effects mediated following depletion of XIST 
      in LSCC cells. Moreover, an miR‑144 target called insulin receptor 
      substrate 1 (IRS1) was significantly decreased by XIST depletion in LSCC cells. 
      IRS1 expression was positively correlated with XIST expression in LSCC tissues. 
      In addition, knockdown of XIST impaired tumor growth in vivo by regulating the 
      miR‑144/IRS1 axis. The present study demonstrated that the progression of LSCC is 
      promoted by XIST sponging miR‑144 to regulate IRS1 expression, suggesting that 
      XIST can serve as a putative target in the therapy of LSCC.
FAU - Cui, Chang-Lei
AU  - Cui CL
AD  - Departments of Anesthesiology, The First Hospital of Jilin University, Changchun, 
      Jilin 130033, P.R. China.
FAU - Li, Yi-Ning
AU  - Li YN
AD  - Department of Otorhinolaryngology‑Head and Neck Surgery, The First Hospital of 
      Jilin University, Changchun, Jilin 130033, P.R. China.
FAU - Cui, Xiang-Yan
AU  - Cui XY
AD  - Department of Otorhinolaryngology‑Head and Neck Surgery, The First Hospital of 
      Jilin University, Changchun, Jilin 130033, P.R. China.
FAU - Wu, Xin
AU  - Wu X
AD  - Department of Otorhinolaryngology‑Head and Neck Surgery, The First Hospital of 
      Jilin University, Changchun, Jilin 130033, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20191216
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (IRS1 protein, human)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (MIRN144 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (XIST non-coding RNA)
SB  - IM
RIN - Oncol Rep. 2021 Dec;46(6):. PMID: 34676882
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Insulin Receptor Substrate Proteins/*genetics/metabolism
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
MH  - Up-Regulation
PMC - PMC6967080
EDAT- 2020/01/03 06:00
MHDA- 2020/08/22 06:00
PMCR- 2019/12/16
CRDT- 2020/01/03 06:00
PHST- 2019/04/09 00:00 [received]
PHST- 2019/11/19 00:00 [accepted]
PHST- 2020/01/03 06:00 [pubmed]
PHST- 2020/08/22 06:00 [medline]
PHST- 2020/01/03 06:00 [entrez]
PHST- 2019/12/16 00:00 [pmc-release]
AID - or-43-02-0525 [pii]
AID - 10.3892/or.2019.7438 [doi]
PST - ppublish
SO  - Oncol Rep. 2020 Feb;43(2):525-535. doi: 10.3892/or.2019.7438. Epub 2019 Dec 16.