PMID- 31898186
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20220928
IS  - 1868-601X (Electronic)
IS  - 1868-4483 (Linking)
VI  - 11
IP  - 5
DP  - 2020 Oct
TI  - Human Milk Oligosaccharide 2'-Fucosyllactose Reduces Neurodegeneration in Stroke 
      Brain.
PG  - 1001-1011
LID - 10.1007/s12975-019-00774-z [doi]
AB  - 2'-Fucosyllactose (2'-FL) is a major oligosaccharide in human milk and is present 
      at trace levels in cow milk. 2'-FL reduces inflammation in the gastrointestinal 
      tract. Its action in the central nervous system has not been well characterized. 
      The purpose of this study is to determine 2'-FL-mediated neural protection and 
      repair in culture and stroke brain. In rat primary cortical neuronal cultures, 
      2'-FL significantly antagonized N-methyl-D-aspartate (NMDA) or glutamate-mediated 
      changes in ATP production, MAP2 immunoreactivity, and TUNEL. The influx of Ca(++) 
      (Ca(++)i) was examined in primary cortical neurons expressing GCaMP5, an 
      endogenous calcium probe. NMDA increased Ca(++)i; 2'-FL significantly attenuated 
      this reaction. In a rat middle cerebral artery occlusion model of stroke, we 
      found that intracerebroventricular pretreatment or oral posttreatment with 2'-FL 
      significantly reduced brain infarction, mitigated microglial activation, improved 
      locomotor activity, and upregulated brain-derived neurotrophic factor (BDNF) 
      expression. Post-stroke delivery of 2'-FL increased bromodeoxyuridine (BrdU) 
      labeling in the perilesioned area. These BrdU cells co-expressed NeuN, or nestin, 
      or GFAP. Using subventricular Matrigel cultures, we demonstrated that 2'-FL 
      increased cell migration from subventricular zone explant. This response was 
      reduced by anti-BDNF blocking antibody. In conclusion, our data suggest that 
      2'-FL has neuroprotective action through inhibition of Ca(++)i, inflammation, and 
      apoptosis. Posttreatment with 2'-FL facilitates neural repair in stroke brain.
FAU - Wu, Kou-Jen
AU  - Wu KJ
AD  - Center for Neuropsychiatric Research, National Health Research Institutes, 
      Zhunan, Miaoli, Taiwan.
FAU - Chen, Yun-Hsiang
AU  - Chen YH
AD  - Department of Life Science, Fu-Jen Catholic University, New Taipei City, Taiwan.
FAU - Bae, Eun-Kyung
AU  - Bae EK
AD  - Center for Neuropsychiatric Research, National Health Research Institutes, 
      Zhunan, Miaoli, Taiwan.
FAU - Song, YoungHa
AU  - Song Y
AD  - Advanced Protein Technologies Corporation, Gyeonggi-Do, South Korea.
FAU - Min, WonKi
AU  - Min W
AD  - Department of Food Science and Development, Kyungil University, Gyeongsan, South 
      Korea.
FAU - Yu, Seong-Jin
AU  - Yu SJ
AUID- ORCID: 0000-0001-7500-5883
AD  - Center for Neuropsychiatric Research, National Health Research Institutes, 
      Zhunan, Miaoli, Taiwan. b7508@nhri.org.tw.
LA  - eng
GR  - 106-2320-B-400 -029 -MY2/Ministry of Science and Technology, Taiwan/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200102
PL  - United States
TA  - Transl Stroke Res
JT  - Translational stroke research
JID - 101517297
RN  - 0 (Oligosaccharides)
RN  - 0 (Trisaccharides)
RN  - XO2533XO8R (2'-fucosyllactose)
SB  - IM
MH  - Animals
MH  - Brain/*drug effects/metabolism
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Male
MH  - Milk, Human/*metabolism
MH  - Neurons/*drug effects/metabolism
MH  - Oligosaccharides/metabolism/*pharmacology
MH  - Rats, Sprague-Dawley
MH  - Trisaccharides/*pharmacology
OTO - NOTNLM
OT  - 2'-Fucosyllactose
OT  - Human milk
OT  - Inflammation
OT  - Neuroprotection
OT  - Stroke
EDAT- 2020/01/04 06:00
MHDA- 2021/07/20 06:00
CRDT- 2020/01/04 06:00
PHST- 2019/09/15 00:00 [received]
PHST- 2019/12/17 00:00 [accepted]
PHST- 2019/12/12 00:00 [revised]
PHST- 2020/01/04 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
PHST- 2020/01/04 06:00 [entrez]
AID - 10.1007/s12975-019-00774-z [pii]
AID - 10.1007/s12975-019-00774-z [doi]
PST - ppublish
SO  - Transl Stroke Res. 2020 Oct;11(5):1001-1011. doi: 10.1007/s12975-019-00774-z. 
      Epub 2020 Jan 2.