PMID- 31898444
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20201123
IS  - 1862-1783 (Electronic)
IS  - 1673-1581 (Print)
IS  - 1673-1581 (Linking)
VI  - 21
IP  - 1
DP  - 2020 Jan.
TI  - Toxicity and metabolism of 3-bromopyruvate in Caenorhabditis elegans.
PG  - 77-86
LID - 10.1631/jzus.B1900370 [doi]
AB  - In this study, we aimed to evaluate the toxic effects, changes in life span, and 
      expression of various metabolism-related genes in Caenorhabditis elegans, using 
      RNA interference (RNAi) and mutant strains, after 3-bromopyruvate (3-BrPA) 
      treatment. C. elegans was treated with various concentrations of 3-BrPA on 
      nematode growth medium (NGM) plates, and their survival was monitored every 24 h. 
      The expression of genes related to metabolism was measured by the real-time 
      fluorescent quantitative polymerase chain reaction (qPCR). Nematode survival in 
      the presence of 3-BrPA was also studied after silencing three hexokinase (HK) 
      genes. The average life span of C. elegans cultured on NGM with 3-BrPA was 
      shortened to 5.7 d compared with 7.7 d in the control group. hxk-1, hxk-2, and 
      hxk-3 were overexpressed after the treatment with 3-BrPA. After successfully 
      interfering hxk-1, hxk-2, and hxk-3, the 50% lethal concentration (LC(50)) of all 
      mutant nematodes decreased with 3-BrPA treatment for 24 h compared with that of 
      the control. All the cyp35 genes tested were overexpressed, except cyp-35B3. The 
      induction of cyp-35A1 expression was most obvious. The LC(50) values of the 
      mutant strains cyp-35A1, cyp-35A2, cyp-35A4, cyp-35B3, and cyp-35C1 were lower 
      than that of the control. Thus, the toxicity of 3-BrPA is closely related to its 
      effect on hexokinase metabolism in nematodes, and the cyp-35 family plays a key 
      role in the metabolism of 3-BrPA.
FAU - Gu, Qiao-Ling
AU  - Gu QL
AD  - The Institute of Minority Physique and Health, Medical College of Northwest 
      University for Nationalities, Lanzhou 730030, China.
AD  - Department of Microbiology, School of Basic Medical Sciences, Guilin Medical 
      University, Guilin 541100, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Anesthesiology, First Hospital, Lanzhou University, Lanzhou 730000, 
      China.
FAU - Fu, Xi-Mei
AU  - Fu XM
AD  - Pathogen Biology Institute, School of Basic Medical Sciences, Lanzhou University, 
      Lanzhou 730000, China.
FAU - Lu, Zhao-Lian
AU  - Lu ZL
AD  - Pathogen Biology Institute, School of Basic Medical Sciences, Lanzhou University, 
      Lanzhou 730000, China.
FAU - Yu, Yao
AU  - Yu Y
AD  - Pathogen Biology Institute, School of Basic Medical Sciences, Lanzhou University, 
      Lanzhou 730000, China.
FAU - Chen, Gen
AU  - Chen G
AD  - Department of Microbiology, School of Basic Medical Sciences, Guilin Medical 
      University, Guilin 541100, China.
FAU - Ma, Rong
AU  - Ma R
AD  - The Institute of Minority Physique and Health, Medical College of Northwest 
      University for Nationalities, Lanzhou 730030, China.
FAU - Kou, Wei
AU  - Kou W
AD  - The Institute of Minority Physique and Health, Medical College of Northwest 
      University for Nationalities, Lanzhou 730030, China.
FAU - Lan, Yong-Mei
AU  - Lan YM
AD  - The Institute of Minority Physique and Health, Medical College of Northwest 
      University for Nationalities, Lanzhou 730030, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Zhejiang Univ Sci B
JT  - Journal of Zhejiang University. Science. B
JID - 101236535
RN  - 0 (CYP35 protein, C elegans)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Pyruvates)
RN  - 0 (RNA, Messenger)
RN  - 63JMV04GRK (bromopyruvate)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*drug effects/metabolism
MH  - Caenorhabditis elegans Proteins/genetics
MH  - Cytochrome P-450 Enzyme System/genetics
MH  - Hexokinase/genetics/physiology
MH  - Pyruvates/metabolism/*toxicity
MH  - RNA, Messenger/analysis
PMC - PMC6964992
OTO - NOTNLM
OT  - 3-Bromopyruvate; Caenorhabditis elegans; Hexokinase; Cytochrome P450
COIS- Compliance with ethics guidelines: Qiao-ling GU, Yan ZHANG, Xi-mei FU, Zhao-lian 
      LU, Yao YU, Gen CHEN, Rong MA, Wei KOU, and Yong-mei LAN declared that they have 
      no conflict of interest. All institutional and national guidelines for the care 
      and use of laboratory animals were followed.
EDAT- 2020/01/04 06:00
MHDA- 2020/11/24 06:00
PMCR- 2020/01/01
CRDT- 2020/01/04 06:00
PHST- 2020/01/04 06:00 [entrez]
PHST- 2020/01/04 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.1631/jzus.B1900370 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci B. 2020 Jan.;21(1):77-86. doi: 10.1631/jzus.B1900370.