PMID- 31902107
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20201019
IS  - 2092-9293 (Electronic)
IS  - 1976-9571 (Linking)
VI  - 42
IP  - 3
DP  - 2020 Mar
TI  - Cytogenetic analysis of Bienertia sinuspersici Akhani as the first step in genome 
      sequencing.
PG  - 337-345
LID - 10.1007/s13258-019-00908-5 [doi]
AB  - BACKGROUND: C(4) plants are efficient in suppressing photorespiration and 
      enhancing carbon gain as compared to C(3) plants. Bienertia sinuspersici Akhani 
      is one of the few species in the family Amaranthaceae that can perform C(4) 
      photosynthesis within individual chlorenchyma cells, without the conventional 
      Kranz anatomy in its leaf. This plant is salt-tolerant and is well-adapted to 
      thrive in hot and humid climates. To date, there have been no reported 
      cytogenetic analyses yet on this species. OBJECTIVE: This study aims to provide a 
      cytogenetic analysis of B. sinuspersici as the first step in genome sequencing. 
      METHODS: Fluorescence in situ hybridization (FISH) karyotype analysis was 
      conducted using the metaphase chromosomes of B. sinuspersici probed with 5S and 
      45S rDNA and Arabidopsis-type telomeric repeats. RESULTS: Results of the 
      cytogenetic analysis confirmed that B. sinuspersici has 2n = 2x = 18 consisting 
      of nine pairs of metacentric chromosomes. Two loci of 45S rDNA were found on the 
      distal regions of the short arm of chromosome 7. Nine loci of 5S rDNA were found 
      in the pericentromeric regions of chromosomes 1, 3, 4, 6, and 8, which also 
      colocalized with Arabidopsis-type telomeric repeats; while four loci in the 
      interstitial regions of chromosome 5 and 8 can be observed. The single locus of 
      5S rDNA that was found in chromosome 8 appears to be hemizygous. CONCLUSION: The 
      FISH karyotype analysis, based on the combination of rDNAs, telomeric tandem 
      repeat markers and C(0)t DNA chromosome landmarks, allowed efficient chromosome 
      identification and provided useful information in characterizing the genome of B. 
      sinuspersici.
FAU - Sevilleno, Samantha Serafin
AU  - Sevilleno SS
AD  - Department of Convergence Science, Sahmyook University, Seoul, 01795, Republic of 
      Korea.
FAU - Ju, Yoon Ha
AU  - Ju YH
AD  - Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, 
      USA.
FAU - Kim, Jung Sun
AU  - Kim JS
AD  - Genetics Division, National Institute of Agricultural Sciences, Rural Development 
      Administration, Jeonju, 54874, Republic of Korea.
FAU - Mancia, Franklin Hinosa
AU  - Mancia FH
AD  - Department of Environmental Horticulture, Sahmyook University, Seoul, 01795, 
      Republic of Korea.
FAU - Byeon, Eun Ju
AU  - Byeon EJ
AD  - Genetics Division, National Institute of Agricultural Sciences, Rural Development 
      Administration, Jeonju, 54874, Republic of Korea.
FAU - Cabahug, Raisa Aone
AU  - Cabahug RA
AD  - Chromosome Research Institute, Sahmyook University, Seoul, 01795, Republic of 
      Korea.
FAU - Hwang, Yoon-Jung
AU  - Hwang YJ
AUID- ORCID: 0000-0002-5298-9282
AD  - Department of Convergence Science, Sahmyook University, Seoul, 01795, Republic of 
      Korea. hyj@syu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200104
PL  - Korea (South)
TA  - Genes Genomics
JT  - Genes & genomics
JID - 101481027
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Amaranthaceae/*cytology/*genetics
MH  - *Chromosome Mapping
MH  - Chromosomes, Plant/genetics
MH  - Cytogenetic Analysis
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotyping
MH  - Telomere/genetics
OTO - NOTNLM
OT  - Bienertia sinuspersici
OT  - C0t DNA
OT  - FISH
OT  - Single-cell C4
OT  - Telomeric repeats
OT  - rDNA
EDAT- 2020/01/07 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/01/06 06:00
PHST- 2019/11/06 00:00 [received]
PHST- 2019/12/18 00:00 [accepted]
PHST- 2020/01/07 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/01/06 06:00 [entrez]
AID - 10.1007/s13258-019-00908-5 [pii]
AID - 10.1007/s13258-019-00908-5 [doi]
PST - ppublish
SO  - Genes Genomics. 2020 Mar;42(3):337-345. doi: 10.1007/s13258-019-00908-5. Epub 
      2020 Jan 4.