PMID- 31909348
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 4
IP  - 27
DP  - 2019 Dec 31
TI  - Preparation of Engineered Extracellular Vesicles Derived from Human Umbilical 
      Cord Mesenchymal Stem Cells with Ultrasonication for Skin Rejuvenation.
PG  - 22638-22645
LID - 10.1021/acsomega.9b03561 [doi]
AB  - Extracellular vesicles (EVs) are lipid-bilayer-enclosed vesicles of submicron 
      size that are secreted by various cells. As mediators of intercellular 
      communication, EVs can alter the physiological state of recipient cells by 
      delivering encapsulated proteins and nucleic acids. Incontestably, growing 
      evidence has shown important biological roles and the clinical relevance of EVs. 
      The use of stem cell-derived EVs as a cell-free therapeutic modality for skin 
      treatment has emerged as a promising application in dermatology. However, the 
      moderate isolation efficiency of prevalent ultracentrifugation and low secretion 
      rate make the massive low-cost production of EVs difficult. Here, we report 
      development of engineered EVs (eEV) derived from human umbilical cord mesenchymal 
      stem cells (hucMSCs) for skin treatment. Ultrasonication was used to shear intact 
      hucMSCs for only 1 min, followed by regular centrifugation and filtration for 
      producing nanoscale eEVs. This approach has  approximately 20-fold higher yield and  approximately 100-fold 
      faster production than that of naturally secreted EVs (nsEV), while the 
      production cost decreased to less than 10%. The eEVs have similar morphology, 
      size distribution, and typical protein markers compared to nsEVs. Moreover, in 
      vitro, both nsEVs and eEVs promote the proliferation and migration of dermal 
      fibroblasts and increase in the expression of collagen, elastin, and fibronectin, 
      whereas the matrix metalloproteinases-1 (MMP-1) and MMP-3 production can be 
      significantly reduced. The wound-healing study in mice showed that both nsEVs and 
      eEVs promote wound recovery in comparison with the controls. In sum, our results 
      indicate that hucMSC-derived eEVs prepared by ultrasonication potentially can be 
      used to increase skin extracellular matrix and enhance skin rejuvenation.
CI  - Copyright (c) 2019 American Chemical Society.
FAU - Wang, Lixue
AU  - Wang L
AD  - Department of Radiotherapy, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer 
      Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 
      Jiangsu 210009, China.
AD  - Department of Radiotherapy, The Second Hospital of Nanjing, Nanjing University of 
      Chinese Medicine, Nanjing, Jiangsu 210003, China.
FAU - Abhange, Komal K
AU  - Abhange KK
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
FAU - Wen, Yi
AU  - Wen Y
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
FAU - Chen, Yundi
AU  - Chen Y
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
FAU - Xue, Fei
AU  - Xue F
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
FAU - Wang, Guosheng
AU  - Wang G
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
FAU - Tong, Jinlong
AU  - Tong J
AD  - Department of Radiotherapy, The Second Hospital of Nanjing, Nanjing University of 
      Chinese Medicine, Nanjing, Jiangsu 210003, China.
FAU - Zhu, Chuandong
AU  - Zhu C
AD  - Department of Radiotherapy, The Second Hospital of Nanjing, Nanjing University of 
      Chinese Medicine, Nanjing, Jiangsu 210003, China.
FAU - He, Xia
AU  - He X
AD  - Department of Radiotherapy, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer 
      Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 
      Jiangsu 210009, China.
FAU - Wan, Yuan
AU  - Wan Y
AD  - The Pq Laboratory of Micro/Nano BiomeDx, Department of Biomedical Engineering, 
      Binghamton University-SUNY, Binghamton, New York 13902, United States.
LA  - eng
PT  - Journal Article
DEP - 20191219
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
EIN - ACS Omega. 2020 Jun 25;5(26):16317. PMID: 32656457
PMC - PMC6941387
COIS- The authors declare no competing financial interest.
EDAT- 2020/01/08 06:00
MHDA- 2020/01/08 06:01
PMCR- 2019/12/19
CRDT- 2020/01/08 06:00
PHST- 2019/10/23 00:00 [received]
PHST- 2019/12/05 00:00 [accepted]
PHST- 2020/01/08 06:00 [entrez]
PHST- 2020/01/08 06:00 [pubmed]
PHST- 2020/01/08 06:01 [medline]
PHST- 2019/12/19 00:00 [pmc-release]
AID - 10.1021/acsomega.9b03561 [doi]
PST - epublish
SO  - ACS Omega. 2019 Dec 19;4(27):22638-22645. doi: 10.1021/acsomega.9b03561. 
      eCollection 2019 Dec 31.