PMID- 31915373
OWN - NLM
STAT- MEDLINE
DCOM- 20200423
LR  - 20210731
IS  - 1476-4679 (Electronic)
IS  - 1465-7392 (Linking)
VI  - 22
IP  - 1
DP  - 2020 Jan
TI  - Single-cell reconstruction of the adult human heart during heart failure and 
      recovery reveals the cellular landscape underlying cardiac function.
PG  - 108-119
LID - 10.1038/s41556-019-0446-7 [doi]
AB  - Owing to the prevalence and high mortality rates of cardiac diseases, a more 
      detailed characterization of the human heart is necessary; however, this has been 
      largely impeded by the cellular diversity of cardiac tissue and limited access to 
      samples. Here, we show transcriptome profiling of 21,422 single cells-including 
      cardiomyocytes (CMs) and non-CMs (NCMs)-from normal, failed and partially 
      recovered (left ventricular assist device treatment) adult human hearts. 
      Comparative analysis of atrial and ventricular cells revealed pronounced inter- 
      and intracompartmental CM heterogeneity as well as compartment-specific 
      utilization of NCM cell types as major cell-communication hubs. Systematic 
      analysis of cellular compositions and cell-cell interaction networks showed that 
      CM contractility and metabolism are the most prominent aspects that are 
      correlated with changes in heart function. We also uncovered active engagement of 
      NCMs in regulating the behaviour of CMs, exemplified by ACKR1(+)-endothelial 
      cells, injection of which preserved cardiac function after injury. Beyond serving 
      as a rich resource, our study provides insights into cell-type-targeted 
      intervention of heart diseases.
FAU - Wang, Li
AU  - Wang L
AUID- ORCID: 0000-0002-5570-1336
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China. wangl@pumc.edu.cn.
FAU - Yu, Peng
AU  - Yu P
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Zhou, Bingying
AU  - Zhou B
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Song, Jiangping
AU  - Song J
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Li, Zheng
AU  - Li Z
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Zhang, Mingzhi
AU  - Zhang M
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Guo, Guangran
AU  - Guo G
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Wang, Yin
AU  - Wang Y
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Chen, Xiao
AU  - Chen X
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China.
FAU - Han, Leng
AU  - Han L
AUID- ORCID: 0000-0002-7380-2640
AD  - Department of Biochemistry and Molecular Biology, The University of Texas Health 
      Science Center at Houston McGovern Medical School, Houston, TX, USA.
FAU - Hu, Shengshou
AU  - Hu S
AUID- ORCID: 0000-0002-7473-6729
AD  - State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center 
      for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Beijing, China. huss@fuwaihospital.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200108
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Endothelial Cells/*metabolism
MH  - Gene Expression Profiling/methods
MH  - Heart Failure/*metabolism
MH  - Heart Ventricles/*metabolism
MH  - Humans
MH  - Myocytes, Cardiac/*metabolism
EDAT- 2020/01/10 06:00
MHDA- 2020/04/24 06:00
CRDT- 2020/01/10 06:00
PHST- 2019/03/21 00:00 [received]
PHST- 2019/11/28 00:00 [accepted]
PHST- 2020/01/10 06:00 [entrez]
PHST- 2020/01/10 06:00 [pubmed]
PHST- 2020/04/24 06:00 [medline]
AID - 10.1038/s41556-019-0446-7 [pii]
AID - 10.1038/s41556-019-0446-7 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2020 Jan;22(1):108-119. doi: 10.1038/s41556-019-0446-7. Epub 2020 
      Jan 8.