PMID- 31918161
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 127
DP  - 2020 Mar
TI  - IkappaBzeta controls NLRP3 inflammasome activation via upregulation of the Nlrp3 gene.
PG  - 154983
LID - S1043-4666(19)30415-6 [pii]
LID - 10.1016/j.cyto.2019.154983 [doi]
AB  - Inflammasome activation induces the maturation and secretion of interleukin 
      (IL)-1beta and -18, and is dependent on NF-kappaB signaling to induce the transcription 
      of the inflammasome components, called the priming step. This study elucidated 
      the role of IkappaBzeta, an atypical IkappaBs (inhibitor of kappaB) and a coactivator of NF-kappaB 
      target genes, on the activation of inflammasome. Bone marrow-derived macrophages 
      (BMDMs) that originated from IkappaBzeta-encoding Nfkbiz gene depletion mice presented a 
      defect in NLRP3 inflammasome activation. In addition, the Nfkbiz(+/-) and 
      Nfkbiz(-/-) mice significantly attenuated serum IL-1beta secretion in response to a 
      monosodium urate injection, a NLRP3 trigger, when compared with Nfkbiz(-+/+) 
      mice. The lack of IkappaBzeta in BMDMs produced a disability in the expression of Nlrp3 
      and pro-Il1beta mRNAs during the priming step. In addition, ectopic IkappaBzeta expression 
      enhanced the Nlrp3 promoter activity, and Nlrp3 and pro-Il1beta transcription. 
      Overall, IkappaBzeta controlled the activation of NLRP3 inflammasome by upregulating the 
      Nlrp3 gene during the priming step.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Kim, Jeongeun
AU  - Kim J
AD  - College of Veterinary Medicine and Institute of Veterinary Science, College of 
      Pharmacy, Kangwon National University, Chuncheon, Gangwon 24341, Republic of 
      Korea.
FAU - Ahn, Huijeong
AU  - Ahn H
AD  - College of Veterinary Medicine and Institute of Veterinary Science, College of 
      Pharmacy, Kangwon National University, Chuncheon, Gangwon 24341, Republic of 
      Korea.
FAU - Yu, Sangjung
AU  - Yu S
AD  - College of Veterinary Medicine and Institute of Veterinary Science, College of 
      Pharmacy, Kangwon National University, Chuncheon, Gangwon 24341, Republic of 
      Korea.
FAU - Ahn, Jae-Hee
AU  - Ahn JH
AD  - Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National 
      University, Chuncheon, Gangwon 24341, Republic of Korea.
FAU - Ko, Hyun-Jeong
AU  - Ko HJ
AD  - Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National 
      University, Chuncheon, Gangwon 24341, Republic of Korea.
FAU - Kweon, Mi-Na
AU  - Kweon MN
AD  - Mucosal Immunology Laboratory, Department of Convergence Medicine, University of 
      Ulsan College of Medicine/Asan Medical Center, Seoul 05505, Republic of Korea.
FAU - Hong, Eui-Ju
AU  - Hong EJ
AD  - College of Veterinary Medicine and Institute of Veterinary Science, Chungnam 
      National University, Daejeon 34134, Republic of Korea.
FAU - An, Beum-Soo
AU  - An BS
AD  - Department of Biomaterial Science, College of Natural Resources and Life Science, 
      Pusan National University, Gyeongsangnam-do 50612, Republic of Korea.
FAU - Lee, Eunsong
AU  - Lee E
AD  - College of Veterinary Medicine and Institute of Veterinary Science, College of 
      Pharmacy, Kangwon National University, Chuncheon, Gangwon 24341, Republic of 
      Korea.
FAU - Lee, Geun-Shik
AU  - Lee GS
AD  - College of Veterinary Medicine and Institute of Veterinary Science, College of 
      Pharmacy, Kangwon National University, Chuncheon, Gangwon 24341, Republic of 
      Korea. Electronic address: leegeun@kangwon.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200107
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nfkbiz protein, mouse)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics
MH  - Animals
MH  - Cells, Cultured
MH  - Inflammasomes/*genetics
MH  - Macrophages/metabolism
MH  - Mice
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*genetics
MH  - Promoter Regions, Genetic/genetics
MH  - RAW 264.7 Cells
MH  - RNA, Messenger/genetics
MH  - Signal Transduction/genetics
MH  - Transcription, Genetic/genetics
MH  - Up-Regulation/*genetics
OTO - NOTNLM
OT  - Inflammasome
OT  - IkappaBzeta
OT  - Macrophages
OT  - Nfkbiz
OT  - Priming step
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/01/10 06:00
MHDA- 2021/08/19 06:00
CRDT- 2020/01/10 06:00
PHST- 2019/08/14 00:00 [received]
PHST- 2019/12/12 00:00 [revised]
PHST- 2019/12/31 00:00 [accepted]
PHST- 2020/01/10 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2020/01/10 06:00 [entrez]
AID - S1043-4666(19)30415-6 [pii]
AID - 10.1016/j.cyto.2019.154983 [doi]
PST - ppublish
SO  - Cytokine. 2020 Mar;127:154983. doi: 10.1016/j.cyto.2019.154983. Epub 2020 Jan 7.