PMID- 31920356
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220412
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 12
DP  - 2019
TI  - Differential Expression of Human N-Alpha-Acetyltransferase 40 (hNAA40), 
      Nicotinamide Phosphoribosyltransferase (NAMPT) and Sirtuin-1 (SIRT-1) Pathway in 
      Obesity and T2DM: Modulation by Metformin and Macronutrient Intake.
PG  - 2765-2774
LID - 10.2147/DMSO.S228591 [doi]
AB  - BACKGROUND: Interactions between environmental factors, such as diet and 
      lifestyle, and metabolic pathways are pivotal in understanding aging mechanisms. 
      hNAA40, Nicotinamide phosphoribosyltransferase (NAMPT), and NAD-dependent protein 
      deacetylase sirtuin-1 (SIRT-1) have been shown to exert important biological 
      processes, including stress response and aging. METHODS: hNAA40, NAMPT, and 
      SIRT-1 mRNA expression in peripheral blood mononuclear cells (PBMC) were 
      quantitated in 30 lean adult volunteers of normal weight, 30 obese, 20 drug-naive 
      obese Type 2 diabetes mellitus (T2DM), and 30 obese T2DM on Metformin. Similarly, 
      hNAA40, NAMPT, and SIRT-1 expression in PBMC were quantitated in 36 normal 
      healthy adults randomly assigned to three different groups (Glucose or Whey 
      proteins or lipids; 300 kcal). Blood samples were obtained at 1, 2, and 3 hrs 
      after the macronutrient intake. RESULTS: There was an increase in hNAA40 and a 
      decrease in NAMPT and SIRT-1 expression in PBMC from T2DM. Metformin treatment 
      reverted hNAA40, NAMPT, and SIRT-1 expression levels to normal levels. Glucose 
      intake resulted in a significant increase in expression of hNAA40 at 1 hr and 
      decreased significantly at 3 hrs post intake. Lipid intake resulted in an 
      increase in expression of hNAA40 at 2 hr post intake and returned to normal 
      levels at 3 hrs. Neither glucose nor lipid intake resulted in a significant 
      change in NAMPT or SIRT-1 expression. Whey proteins resulted in significantly 
      lower expression of NAMPT at 3 hrs and did not alter the expression levels of 
      SIRT-1 significantly. CONCLUSION: hNAA40, NAMPT, and SIRT-1 pathway could play a 
      role in the determination of the healthy life-span. Metformin modulates this 
      pathway.
CI  - (c) 2019 Alshahrani et al.
FAU - Alshahrani, Awad
AU  - Alshahrani A
AD  - Department of Medicine, Ministry of National Guard Health Affairs (MNG-HA), 
      Riyadh, Kingdom of Saudi Arabia.
AD  - King Abdullah International Medical Research Centre (KAIMRC), Riyadh, Kingdom of 
      Saudi Arabia.
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - AlDubayee, Mohammed
AU  - AlDubayee M
AD  - Department of Medicine, Ministry of National Guard Health Affairs (MNG-HA), 
      Riyadh, Kingdom of Saudi Arabia.
AD  - King Abdullah International Medical Research Centre (KAIMRC), Riyadh, Kingdom of 
      Saudi Arabia.
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Zahra, Mahmoud
AU  - Zahra M
AUID- ORCID: 0000-0001-5067-4984
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Alsebayel, Firas M
AU  - Alsebayel FM
AUID- ORCID: 0000-0001-5053-7410
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Alammari, Nawaf
AU  - Alammari N
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Alsudairy, Faisal
AU  - Alsudairy F
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Almajed, Muath
AU  - Almajed M
AD  - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, 
      Riyadh, Kingdom of Saudi Arabia.
FAU - Aljada, Ahmad
AU  - Aljada A
AUID- ORCID: 0000-0001-8337-5454
AD  - Department of Biochemistry and Molecular Medicine, College of Medicine, Alfaisal 
      University, Riyadh, Kingdom of Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20191227
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC6938199
OTO - NOTNLM
OT  - NAMPT
OT  - T2DM
OT  - hNAA40
OT  - nicotinamide phosphoribosyltransferase
OT  - obesity
OT  - senescence
OT  - sirtuin-1
COIS- All authors declare no conflicts of interest in relation to the work reported in 
      this manuscript.
EDAT- 2020/01/11 06:00
MHDA- 2020/01/11 06:01
PMCR- 2019/12/27
CRDT- 2020/01/11 06:00
PHST- 2019/08/24 00:00 [received]
PHST- 2019/11/27 00:00 [accepted]
PHST- 2020/01/11 06:00 [entrez]
PHST- 2020/01/11 06:00 [pubmed]
PHST- 2020/01/11 06:01 [medline]
PHST- 2019/12/27 00:00 [pmc-release]
AID - 228591 [pii]
AID - 10.2147/DMSO.S228591 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2019 Dec 27;12:2765-2774. doi: 10.2147/DMSO.S228591. 
      eCollection 2019.