PMID- 31921135
OWN - NLM
STAT- MEDLINE
DCOM- 20201104
LR  - 20201104
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Heme-Oxygenase-1 Is Decreased in Circulating Monocytes and Is Associated With 
      Impaired Phagocytosis and ROS Production in Lupus Nephritis.
PG  - 2868
LID - 10.3389/fimmu.2019.02868 [doi]
LID - 2868
AB  - Lupus nephritis (LN) is one of the most serious manifestations of systemic lupus 
      erythematosus (SLE). Based on studies showing the potential role of heme 
      oxygenase-1 (HO-1), an enzyme that catalyzes the degradation of heme and has 
      anti-inflammatory properties in SLE development, we decided to explore HO-1 in 
      LN. Accordingly, we evaluated HO-1 levels and function in circulating and 
      infiltrating monocytes and neutrophils of LN patients. HO-1 levels were assessed 
      in peripheral monocytes of LN patients and controls by flow cytometry and 
      immunofluorescence microscopy. Phagocytosis and the production of reactive oxygen 
      species (ROS) were evaluated to determine the effect of HO-1 in monocyte 
      function. In addition, renal biopsies with proliferative LN were used to identify 
      HO-1 in infiltrating cells and renal tissue by immunofluorescence and 
      immunohistochemistry. Biopsies of healthy controls (HC) and patients who 
      underwent nephrectomy were included as controls. Circulating pro-inflammatory 
      monocytes and activated neutrophils were increased in LN patients. HO-1 levels 
      were decreased in all subsets of monocytes and in activated neutrophils. LN 
      monocytes showed increased phagocytosis and higher production of ROS than those 
      of HC. When HO-1 was induced, phagocytosis and ROS levels became similar to those 
      of HC. HO-1 was mostly expressed in renal tubular epithelial cells (RTEC). Renal 
      tissue of LN patients showed lower levels of HO-1 than HC, whereas infiltrating 
      immune cells of LN showed lower levels of HO-1 than biopsies of patients who had 
      renal surgery. HO-1 is decreased in circulating monocytes and activated 
      neutrophils of LN patients. HO-1 levels modulate the phagocytosis of LN monocytes 
      and ROS production. HO-1 expression in RTEC might be an attempt of 
      self-protection from inflammation.
CI  - Copyright (c) 2019 Cuitino, Obreque, Gajardo-Meneses, Villarroel, Crisostomo, San 
      Francisco, Valenzuela, Mendez and Llanos.
FAU - Cuitino, Loreto
AU  - Cuitino L
AD  - Departamento de Inmunologia Clinica y Reumatologia, Escuela de Medicina, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
FAU - Obreque, Javiera
AU  - Obreque J
AD  - Departamento de Inmunologia Clinica y Reumatologia, Escuela de Medicina, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
FAU - Gajardo-Meneses, Patricia
AU  - Gajardo-Meneses P
AD  - Departamento de Inmunologia Clinica y Reumatologia, Escuela de Medicina, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
FAU - Villarroel, Alejandra
AU  - Villarroel A
AD  - Departamento de Anatomia Patologica, Escuela de Medicina, Pontificia Universidad 
      Catolica de Chile, Santiago, Chile.
FAU - Crisostomo, Natalia
AU  - Crisostomo N
AD  - Departamento de Inmunologia Clinica y Reumatologia, Escuela de Medicina, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
FAU - San Francisco, Ignacio F
AU  - San Francisco IF
AD  - Departamento de Urologia, Escuela de Medicina, Pontificia Universidad Catolica de 
      Chile, Santiago, Chile.
FAU - Valenzuela, Rodrigo A
AU  - Valenzuela RA
AD  - Departamento de Ciencias Quimicas y Biologicas, Facultad de Salud, Universidad 
      Bernardo O'Higgins, Santiago, Chile.
FAU - Mendez, Gonzalo P
AU  - Mendez GP
AD  - Departamento de Anatomia Patologica, Escuela de Medicina, Pontificia Universidad 
      Catolica de Chile, Santiago, Chile.
FAU - Llanos, Carolina
AU  - Llanos C
AD  - Departamento de Inmunologia Clinica y Reumatologia, Escuela de Medicina, 
      Pontificia Universidad Catolica de Chile, Santiago, Chile.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191213
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Female
MH  - Heme Oxygenase-1/*immunology
MH  - Humans
MH  - Kidney/immunology/pathology
MH  - Lupus Nephritis/*immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology/pathology
MH  - *Phagocytosis
MH  - Reactive Oxygen Species/*immunology
PMC - PMC6923251
OTO - NOTNLM
OT  - heme oxygenase-1 (HO-1)
OT  - lupus nephritis
OT  - monocytes
OT  - phagocytosis
OT  - reactive oxygen species (ROS)
OT  - renal biopsies
EDAT- 2020/01/11 06:00
MHDA- 2020/11/05 06:00
PMCR- 2019/01/01
CRDT- 2020/01/11 06:00
PHST- 2019/05/30 00:00 [received]
PHST- 2019/11/22 00:00 [accepted]
PHST- 2020/01/11 06:00 [entrez]
PHST- 2020/01/11 06:00 [pubmed]
PHST- 2020/11/05 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.02868 [doi]
PST - epublish
SO  - Front Immunol. 2019 Dec 13;10:2868. doi: 10.3389/fimmu.2019.02868. eCollection 
      2019.