PMID- 31921167 OWN - NLM STAT- MEDLINE DCOM- 20201106 LR - 20201106 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 10 DP - 2019 TI - Human Apoptotic Cells, Generated by Extracorporeal Photopheresis, Modulate Allogeneic Immune Response. PG - 2908 LID - 10.3389/fimmu.2019.02908 [doi] LID - 2908 AB - The induction of specific and sustainable tolerance is a challenging issue in organ transplantation. The discovery of the immunosuppressive properties of apoptotic cells in animal models has paved the way for their use in human transplantation. In this work, we aimed to define a stable, reproducible, and clinically compatible production procedure of human apoptotic cells (Apo-cells). Using a clinically approved extracorporeal photopheresis technique, we have produced and characterized phenotypically and functionally human apoptotic cells. These Apo-cells have immunosuppressive properties proved in vitro and in vivo in NOD/SCID/gammaC mice by their capacity to modulate an allogeneic response following both a direct and an indirect antigen presentation. These results brought the rationale for the use of Apo-cells in tolerance induction protocol for organ transplantation. CI - Copyright (c) 2019 Pilon, Stehle, Beldi-Ferchiou, Matignon, Thiolat, Burlion, Grondin, Birebent, Pirenne, Rouard, Lang, Marodon, Grimbert and Cohen. FAU - Pilon, Caroline AU - Pilon C AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. FAU - Stehle, Thomas AU - Stehle T AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - AP-HP, Groupe Hospitalo-Universitaire Chenevier Mondor, Service de Nephrologie-Transplantation, Creteil, France. FAU - Beldi-Ferchiou, Asma AU - Beldi-Ferchiou A AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. FAU - Matignon, Marie AU - Matignon M AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. AD - AP-HP, Groupe Hospitalo-Universitaire Chenevier Mondor, Service de Nephrologie-Transplantation, Creteil, France. FAU - Thiolat, Allan AU - Thiolat A AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. FAU - Burlion, Aude AU - Burlion A AD - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France. FAU - Grondin, Cynthia AU - Grondin C AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. FAU - Birebent, Brigitte AU - Birebent B AD - Etablissement Francais du Sang (EFS) - Ile de France, Creteil, France. FAU - Pirenne, France AU - Pirenne F AD - Etablissement Francais du Sang (EFS) - Ile de France, Creteil, France. AD - Inserm, U955, Equipe 2, Creteil, France. FAU - Rouard, Helene AU - Rouard H AD - Etablissement Francais du Sang (EFS) - Ile de France, Creteil, France. FAU - Lang, Philippe AU - Lang P AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. AD - AP-HP, Groupe Hospitalo-Universitaire Chenevier Mondor, Service de Nephrologie-Transplantation, Creteil, France. FAU - Marodon, Gilles AU - Marodon G AD - Sorbonne Universites, UPMC Univ Paris 06, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France. FAU - Grimbert, Philippe AU - Grimbert P AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. AD - AP-HP, Groupe Hospitalo-Universitaire Chenevier Mondor, Service de Nephrologie-Transplantation, Creteil, France. FAU - Cohen, Jose L AU - Cohen JL AD - Assistance Publique-Hopitaux de Paris (AP-HP), Groupe Hospitalo-Universitaire Chenevier Mondor, Centre d'Investigation Clinique Biotherapie, Creteil, France. AD - Institut Mondor de recherche biomedicale, Universite Paris-Est, UMR_S955, UPEC, Creteil, France. AD - Inserm, U955, Equipe 21, Creteil, France. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191218 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 SB - IM MH - Animals MH - *Antigen Presentation MH - Apoptosis/*immunology MH - Humans MH - *Immune Tolerance MH - Mice MH - Mice, Inbred NOD MH - Mice, SCID MH - *Photopheresis PMC - PMC6930166 OTO - NOTNLM OT - NSG mice OT - apoptotic cell OT - immunomodulation OT - tolerance OT - transplantation EDAT- 2020/01/11 06:00 MHDA- 2020/11/11 06:00 PMCR- 2019/01/01 CRDT- 2020/01/11 06:00 PHST- 2019/07/12 00:00 [received] PHST- 2019/11/26 00:00 [accepted] PHST- 2020/01/11 06:00 [entrez] PHST- 2020/01/11 06:00 [pubmed] PHST- 2020/11/11 06:00 [medline] PHST- 2019/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2019.02908 [doi] PST - epublish SO - Front Immunol. 2019 Dec 18;10:2908. doi: 10.3389/fimmu.2019.02908. eCollection 2019.