PMID- 31921620
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 9
DP  - 2019
TI  - Novel Breast-Specific Long Non-coding RNA LINC00993 Acts as a Tumor Suppressor in 
      Triple-Negative Breast Cancer.
PG  - 1325
LID - 10.3389/fonc.2019.01325 [doi]
LID - 1325
AB  - Background: Triple-negative breast cancer (TNBC) was characterized by breast 
      cancers that do not express estrogen receptor (ER), progesterone receptor (PR), 
      or human epidermal growth factor receptor (HER)-2 genes. TNBC patients are 
      associated with a shorter median time to relapse and death for the lack of 
      available treatment targets. Long non-coding RNAs (LncRNAs) have been reported to 
      play an important role in the development of TNBC. We identified a novel 
      breast-specific long non-coding RNA LINC00993, but less was known about its 
      expression pattern and functional role in TNBC. Methods: LINC00993 RNA expression 
      was detected across different types of clinical breast cancer samples by using 
      qRT-PCR. Bioinformatic methods "guilt by association" and gene set enrichment 
      analysis (GSEA) were used to predict LINC00993 functions. Subcellular 
      localization of LINC00993 in cells was detected by RNA fluorescence in situ 
      hybridization (FISH). Effect of LINC00993 on cell growth was measured by plate 
      colony formation assays, typical growth curve, and an in vivo tumor model. Cell 
      cycle analysis was done by flow cytometry analysis. Key cell cycle regulators 
      were detected by Western blot. Results: LINC00993 was largely downregulated in 
      TNBC, and higher expression indicated better outcome. LINC00993 located mainly in 
      the nucleus. LINC00993 suppressed TNBC growth both in vitro and in vivo. 
      LINC00993 was predicted to be involved in cell cycle pathways by using "guilt by 
      association" and GSEA methods. Key cell cycle regulators like p16(INK4A), 
      p14(ARF), p53, and p21 were affected by LINC00993 overexpression. Conclusions: A 
      new breast-specific lincRNA LINC00993 was identified with a tumor-suppressive 
      feature and with prognostic value. This is the first research on LINC00993 
      function. Our results suggest that controlling LINC00993 level may be beneficial 
      for breast cancer treatment.
CI  - Copyright (c) 2019 Guo, Jian, Tao, Chen, Yu and Liu.
FAU - Guo, Shipeng
AU  - Guo S
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Jian, Lei
AU  - Jian L
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Tao, Kai
AU  - Tao K
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Yu, Haochen
AU  - Yu H
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Liu, Shengchun
AU  - Liu S
AD  - Department of Endocrine Breast Surgery, The First Affiliated Hospital of 
      Chongqing Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20191217
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC6928780
OTO - NOTNLM
OT  - LINC00993
OT  - LncRNA
OT  - TNBC
OT  - cell cycle
OT  - guilt by association
EDAT- 2020/01/11 06:00
MHDA- 2020/01/11 06:01
PMCR- 2019/01/01
CRDT- 2020/01/11 06:00
PHST- 2019/07/11 00:00 [received]
PHST- 2019/11/13 00:00 [accepted]
PHST- 2020/01/11 06:00 [entrez]
PHST- 2020/01/11 06:00 [pubmed]
PHST- 2020/01/11 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2019.01325 [doi]
PST - epublish
SO  - Front Oncol. 2019 Dec 17;9:1325. doi: 10.3389/fonc.2019.01325. eCollection 2019.